Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5800 and Pf1N1B4_5801 overlap by 27 nucleotides Pf1N1B4_5801 and Pf1N1B4_5802 are separated by 146 nucleotides
Pf1N1B4_5800 - Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10), at 235,274 to 236,638
_5800
Pf1N1B4_5801 - hypothetical protein, at 236,612 to 236,746
_5801
Pf1N1B4_5802 - benABC operon transcriptional activator BenR, at 236,893 to 237,744
_5802
Position (kb) 236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 235.632 kb on + strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.633 kb on - strand, within Pf1N1B4_5800 at 235.707 kb on + strand, within Pf1N1B4_5800 at 235.803 kb on + strand, within Pf1N1B4_5800 at 235.870 kb on - strand, within Pf1N1B4_5800 at 235.870 kb on - strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.037 kb on + strand, within Pf1N1B4_5800 at 236.038 kb on - strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.043 kb on + strand, within Pf1N1B4_5800 at 236.044 kb on - strand, within Pf1N1B4_5800 at 236.044 kb on - strand, within Pf1N1B4_5800 at 236.322 kb on + strand, within Pf1N1B4_5800 at 236.322 kb on + strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand, within Pf1N1B4_5800 at 236.323 kb on - strand at 236.386 kb on + strand, within Pf1N1B4_5800 at 236.454 kb on + strand, within Pf1N1B4_5800 at 236.497 kb on - strand, within Pf1N1B4_5800 at 236.577 kb on + strand at 236.577 kb on + strand at 236.619 kb on + strand at 236.723 kb on + strand, within Pf1N1B4_5801 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.046 kb on - strand, within Pf1N1B4_5802 at 237.072 kb on - strand, within Pf1N1B4_5802 at 237.113 kb on + strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.192 kb on - strand, within Pf1N1B4_5802 at 237.294 kb on - strand at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.507 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.564 kb on - strand, within Pf1N1B4_5802 at 237.623 kb on + strand, within Pf1N1B4_5802 at 237.624 kb on - strand, within Pf1N1B4_5802 at 237.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 235,632 + Pf1N1B4_5800 0.26 +0.1 235,633 - Pf1N1B4_5800 0.26 +2.1 235,633 - Pf1N1B4_5800 0.26 +0.9 235,707 + Pf1N1B4_5800 0.32 -1.1 235,803 + Pf1N1B4_5800 0.39 -2.5 235,870 - Pf1N1B4_5800 0.44 -1.9 235,870 - Pf1N1B4_5800 0.44 -0.2 236,037 + Pf1N1B4_5800 0.56 +2.5 236,037 + Pf1N1B4_5800 0.56 -0.1 236,037 + Pf1N1B4_5800 0.56 +1.1 236,038 - Pf1N1B4_5800 0.56 +0.3 236,043 + Pf1N1B4_5800 0.56 +0.1 236,043 + Pf1N1B4_5800 0.56 -1.1 236,043 + Pf1N1B4_5800 0.56 -0.7 236,043 + Pf1N1B4_5800 0.56 +0.9 236,043 + Pf1N1B4_5800 0.56 -0.9 236,043 + Pf1N1B4_5800 0.56 -0.1 236,043 + Pf1N1B4_5800 0.56 +0.4 236,044 - Pf1N1B4_5800 0.56 +0.3 236,044 - Pf1N1B4_5800 0.56 +0.5 236,322 + Pf1N1B4_5800 0.77 -1.5 236,322 + Pf1N1B4_5800 0.77 +0.1 236,323 - Pf1N1B4_5800 0.77 +1.4 236,323 - Pf1N1B4_5800 0.77 -0.1 236,323 - Pf1N1B4_5800 0.77 -2.5 236,323 - Pf1N1B4_5800 0.77 -0.2 236,323 - Pf1N1B4_5800 0.77 -0.6 236,323 - +2.1 236,386 + Pf1N1B4_5800 0.81 +1.5 236,454 + Pf1N1B4_5800 0.86 -0.0 236,497 - Pf1N1B4_5800 0.90 -0.8 236,577 + +1.5 236,577 + +0.5 236,619 + +1.3 236,723 + Pf1N1B4_5801 0.82 +0.7 237,046 - Pf1N1B4_5802 0.18 +0.2 237,046 - Pf1N1B4_5802 0.18 -1.1 237,046 - Pf1N1B4_5802 0.18 -1.2 237,072 - Pf1N1B4_5802 0.21 -0.5 237,113 + Pf1N1B4_5802 0.26 -1.8 237,192 - Pf1N1B4_5802 0.35 +0.4 237,192 - Pf1N1B4_5802 0.35 +0.0 237,294 - -0.5 237,507 - Pf1N1B4_5802 0.72 -2.1 237,507 - Pf1N1B4_5802 0.72 -1.0 237,507 - Pf1N1B4_5802 0.72 -1.5 237,507 - Pf1N1B4_5802 0.72 +2.0 237,564 - Pf1N1B4_5802 0.79 +0.2 237,564 - Pf1N1B4_5802 0.79 +1.4 237,564 - Pf1N1B4_5802 0.79 +1.5 237,564 - Pf1N1B4_5802 0.79 +0.0 237,623 + Pf1N1B4_5802 0.86 +1.1 237,624 - Pf1N1B4_5802 0.86 -0.3 237,648 + -3.1
Or see this region's nucleotide sequence