Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5644 and Pf1N1B4_5645 are separated by 52 nucleotides Pf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotides Pf1N1B4_5646 and Pf1N1B4_5647 are separated by 197 nucleotides Pf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides
Pf1N1B4_5644 - amino acid ABC transporter, permease protein, at 73,576 to 74,238
_5644
Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136
_5645
Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280
_5646
Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927
_5647
Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465
_5648
Position (kb) 75
76 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 74.231 kb on + strand at 74.232 kb on - strand at 74.232 kb on - strand at 74.248 kb on + strand at 74.248 kb on + strand at 74.249 kb on - strand at 74.249 kb on - strand at 74.367 kb on + strand at 74.461 kb on - strand, within Pf1N1B4_5645 at 74.461 kb on - strand at 74.523 kb on + strand, within Pf1N1B4_5645 at 74.524 kb on - strand at 74.584 kb on - strand, within Pf1N1B4_5645 at 74.656 kb on - strand, within Pf1N1B4_5645 at 74.721 kb on + strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.999 kb on - strand, within Pf1N1B4_5645 at 75.188 kb on - strand, within Pf1N1B4_5646 at 75.577 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.626 kb on - strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.647 kb on - strand, within Pf1N1B4_5647 at 75.712 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 76.062 kb on + strand at 76.062 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 74,231 + +0.3 74,232 - -0.5 74,232 - +0.5 74,248 + -0.6 74,248 + +0.1 74,249 - +0.9 74,249 - +0.8 74,367 + -0.7 74,461 - Pf1N1B4_5645 0.20 +0.1 74,461 - +0.5 74,523 + Pf1N1B4_5645 0.27 -0.1 74,524 - -0.2 74,584 - Pf1N1B4_5645 0.35 +1.3 74,656 - Pf1N1B4_5645 0.43 +0.5 74,721 + Pf1N1B4_5645 0.51 -1.1 74,722 - Pf1N1B4_5645 0.51 -2.7 74,722 - Pf1N1B4_5645 0.51 +2.3 74,999 - Pf1N1B4_5645 0.84 +2.8 75,188 - Pf1N1B4_5646 0.38 -2.1 75,577 + Pf1N1B4_5647 0.22 -1.7 75,625 + Pf1N1B4_5647 0.33 +1.8 75,625 + Pf1N1B4_5647 0.33 +0.8 75,625 + Pf1N1B4_5647 0.33 +0.5 75,626 - Pf1N1B4_5647 0.33 -0.1 75,627 + Pf1N1B4_5647 0.33 -2.1 75,627 + Pf1N1B4_5647 0.33 +0.8 75,627 + Pf1N1B4_5647 0.33 -0.3 75,627 + Pf1N1B4_5647 0.33 -1.5 75,627 + Pf1N1B4_5647 0.33 -1.8 75,647 - Pf1N1B4_5647 0.38 -0.5 75,712 + Pf1N1B4_5647 0.52 +0.2 75,869 + Pf1N1B4_5647 0.87 -0.7 75,869 + Pf1N1B4_5647 0.87 -1.8 76,062 + +0.8 76,062 + +0.9
Or see this region's nucleotide sequence