Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5448 and Pf1N1B4_5449 are separated by 312 nucleotides Pf1N1B4_5449 and Pf1N1B4_5450 overlap by 25 nucleotides
Pf1N1B4_5448 - hypothetical protein, at 194,385 to 194,507
_5448
Pf1N1B4_5449 - Error-prone repair protein UmuD, at 194,820 to 195,131
_5449
Pf1N1B4_5450 - Error-prone, lesion bypass DNA polymerase V (UmuC), at 195,107 to 196,402
_5450
Position (kb) 194
195 Strain fitness (log2 ratio) -2
-1
0
1
2 at 193.399 kb on + strand at 193.449 kb on + strand at 193.450 kb on - strand at 193.552 kb on - strand at 193.552 kb on - strand at 193.836 kb on + strand at 193.836 kb on + strand at 193.837 kb on - strand at 193.837 kb on - strand at 193.873 kb on - strand at 193.879 kb on + strand at 193.880 kb on - strand at 193.880 kb on - strand at 193.889 kb on - strand at 193.889 kb on - strand at 193.889 kb on - strand at 194.054 kb on + strand at 194.055 kb on - strand at 194.055 kb on - strand at 194.176 kb on + strand at 194.176 kb on + strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.177 kb on - strand at 194.278 kb on - strand at 194.278 kb on - strand at 194.328 kb on + strand at 194.328 kb on + strand at 194.329 kb on - strand at 194.440 kb on + strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.441 kb on - strand at 194.441 kb on - strand, within Pf1N1B4_5448 at 194.495 kb on + strand at 194.496 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.543 kb on - strand at 194.601 kb on + strand at 194.602 kb on - strand at 194.672 kb on - strand at 194.709 kb on - strand at 194.709 kb on - strand at 194.730 kb on + strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.731 kb on - strand at 194.749 kb on + strand at 194.750 kb on - strand at 194.798 kb on + strand at 194.798 kb on + strand at 194.799 kb on - strand at 194.799 kb on - strand at 195.050 kb on + strand, within Pf1N1B4_5449 at 195.051 kb on - strand, within Pf1N1B4_5449 at 195.173 kb on + strand at 195.174 kb on - strand at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.282 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.345 kb on + strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.346 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.349 kb on - strand, within Pf1N1B4_5450 at 195.379 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.441 kb on + strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.442 kb on - strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.456 kb on + strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450 at 195.457 kb on - strand, within Pf1N1B4_5450
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 193,399 + +0.1 193,449 + -1.4 193,450 - +0.5 193,552 - -0.7 193,552 - +0.5 193,836 + -0.9 193,836 + -0.5 193,837 - +0.4 193,837 - -0.2 193,873 - -0.9 193,879 + +0.7 193,880 - -0.5 193,880 - +2.4 193,889 - +0.8 193,889 - -1.9 193,889 - -0.2 194,054 + -1.3 194,055 - +0.3 194,055 - -0.6 194,176 + -0.2 194,176 + -0.5 194,177 - +0.1 194,177 - +0.1 194,177 - +1.0 194,177 - +0.7 194,278 - +1.6 194,278 - +0.4 194,328 + +0.3 194,328 + -2.2 194,329 - -0.5 194,440 + Pf1N1B4_5448 0.45 +1.9 194,441 - Pf1N1B4_5448 0.46 -1.1 194,441 - Pf1N1B4_5448 0.46 -1.6 194,441 - Pf1N1B4_5448 0.46 -0.5 194,441 - +0.1 194,441 - Pf1N1B4_5448 0.46 -1.7 194,495 + +2.1 194,496 - -0.3 194,543 - -0.6 194,543 - +0.1 194,543 - -0.9 194,601 + -2.2 194,602 - +1.1 194,672 - +0.1 194,709 - +1.0 194,709 - -1.5 194,730 + +1.3 194,731 - +1.1 194,731 - -0.3 194,731 - -0.9 194,749 + +0.5 194,750 - +1.3 194,798 + -1.9 194,798 + -0.1 194,799 - +0.4 194,799 - +0.1 195,050 + Pf1N1B4_5449 0.74 -0.5 195,051 - Pf1N1B4_5449 0.74 +0.7 195,173 + -0.9 195,174 - +0.9 195,282 + Pf1N1B4_5450 0.14 -0.1 195,282 + Pf1N1B4_5450 0.14 +0.7 195,345 + +1.1 195,345 + Pf1N1B4_5450 0.18 +0.1 195,345 + Pf1N1B4_5450 0.18 +0.1 195,345 + Pf1N1B4_5450 0.18 +0.5 195,346 - Pf1N1B4_5450 0.18 -0.3 195,346 - Pf1N1B4_5450 0.18 +1.1 195,346 - Pf1N1B4_5450 0.18 -0.9 195,349 - Pf1N1B4_5450 0.19 -0.9 195,349 - Pf1N1B4_5450 0.19 +0.9 195,379 + Pf1N1B4_5450 0.21 -0.5 195,441 + Pf1N1B4_5450 0.26 -0.1 195,441 + Pf1N1B4_5450 0.26 +0.1 195,441 + Pf1N1B4_5450 0.26 +0.1 195,442 - Pf1N1B4_5450 0.26 +0.1 195,442 - Pf1N1B4_5450 0.26 -0.9 195,456 + Pf1N1B4_5450 0.27 +0.1 195,456 + Pf1N1B4_5450 0.27 +1.1 195,456 + Pf1N1B4_5450 0.27 +0.7 195,456 + Pf1N1B4_5450 0.27 -0.9 195,456 + Pf1N1B4_5450 0.27 +0.4 195,457 - Pf1N1B4_5450 0.27 -0.2 195,457 - Pf1N1B4_5450 0.27 +0.5 195,457 - Pf1N1B4_5450 0.27 +0.4
Or see this region's nucleotide sequence