Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_515

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_514 and Pf1N1B4_515 are separated by 256 nucleotidesPf1N1B4_515 and Pf1N1B4_516 overlap by 1 nucleotides Pf1N1B4_514 - Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23), at 605,492 to 606,226 _514 Pf1N1B4_515 - DNA-binding response regulator GltR, controls specific porins for the entry of glucose, at 606,483 to 607,241 _515 Pf1N1B4_516 - Sensor histidine kinase, at 607,241 to 608,293 _516 Position (kb) 606 607 608Strain fitness (log2 ratio) -1 0 1 2 3at 605.483 kb on + strandat 605.493 kb on + strandat 606.294 kb on + strandat 606.294 kb on + strandat 606.295 kb on - strandat 606.415 kb on + strandat 606.416 kb on - strandat 606.441 kb on + strandat 606.441 kb on + strandat 606.441 kb on + strandat 606.442 kb on - strandat 606.461 kb on + strandat 606.461 kb on + strandat 606.461 kb on + strandat 606.536 kb on + strandat 606.604 kb on - strand, within Pf1N1B4_515at 606.621 kb on - strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.837 kb on + strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strandat 606.838 kb on - strand, within Pf1N1B4_515at 606.838 kb on - strand, within Pf1N1B4_515at 606.955 kb on + strandat 607.213 kb on - strandat 607.213 kb on - strandat 607.258 kb on + strandat 607.302 kb on + strandat 607.302 kb on + strandat 607.303 kb on - strandat 607.832 kb on + strand, within Pf1N1B4_516at 607.832 kb on + strand, within Pf1N1B4_516at 607.832 kb on + strandat 607.832 kb on + strand, within Pf1N1B4_516at 607.833 kb on - strandat 607.833 kb on - strand, within Pf1N1B4_516at 608.158 kb on + strand, within Pf1N1B4_516at 608.158 kb on + strand, within Pf1N1B4_516

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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605,483 + +2.9
605,493 + +0.3
606,294 + -1.1
606,294 + +0.9
606,295 - -1.0
606,415 + +1.7
606,416 - +0.7
606,441 + +1.3
606,441 + -0.1
606,441 + +0.9
606,442 - +1.5
606,461 + +1.5
606,461 + -0.5
606,461 + +2.3
606,536 + +0.5
606,604 - Pf1N1B4_515 0.16 -1.1
606,621 - Pf1N1B4_515 0.18 +0.3
606,837 + Pf1N1B4_515 0.47 -0.4
606,837 + Pf1N1B4_515 0.47 +1.1
606,837 + Pf1N1B4_515 0.47 +0.5
606,837 + Pf1N1B4_515 0.47 +2.3
606,838 - Pf1N1B4_515 0.47 +0.9
606,838 - Pf1N1B4_515 0.47 -0.4
606,838 - -1.6
606,838 - Pf1N1B4_515 0.47 -0.1
606,838 - Pf1N1B4_515 0.47 +1.4
606,955 + +0.3
607,213 - +1.1
607,213 - +3.1
607,258 + -0.1
607,302 + +0.7
607,302 + -0.1
607,303 - +0.4
607,832 + Pf1N1B4_516 0.56 -0.1
607,832 + Pf1N1B4_516 0.56 -1.5
607,832 + -1.1
607,832 + Pf1N1B4_516 0.56 +0.2
607,833 - -0.1
607,833 - Pf1N1B4_516 0.56 +0.7
608,158 + Pf1N1B4_516 0.87 +1.5
608,158 + Pf1N1B4_516 0.87 +1.9

Or see this region's nucleotide sequence