Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_48 and Pf1N1B4_49 are separated by 30 nucleotides Pf1N1B4_49 and Pf1N1B4_50 are separated by 163 nucleotides Pf1N1B4_50 and Pf1N1B4_51 are separated by 89 nucleotides Pf1N1B4_51 and Pf1N1B4_52 are separated by 337 nucleotides Pf1N1B4_52 and Pf1N1B4_53 are separated by 6 nucleotides
Pf1N1B4_48 - hypothetical protein, at 55,996 to 56,406
_48
Pf1N1B4_49 - hypothetical protein, at 56,437 to 56,643
_49
Pf1N1B4_50 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 56,807 to 56,989
_50
Pf1N1B4_51 - hypothetical protein, at 57,079 to 57,348
_51
Pf1N1B4_52 - FIG00956056: hypothetical protein, at 57,686 to 58,144
_52
Pf1N1B4_53 - hypothetical protein, at 58,151 to 58,342
_53
Position (kb) 57
58 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.516 kb on + strand, within Pf1N1B4_49 at 56.517 kb on - strand, within Pf1N1B4_49 at 56.520 kb on - strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.559 kb on + strand, within Pf1N1B4_49 at 56.560 kb on - strand, within Pf1N1B4_49 at 56.560 kb on - strand at 56.560 kb on - strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.588 kb on + strand, within Pf1N1B4_49 at 56.767 kb on + strand at 56.784 kb on + strand at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.837 kb on + strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand, within Pf1N1B4_50 at 56.838 kb on - strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.895 kb on + strand, within Pf1N1B4_50 at 56.896 kb on - strand at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 56.896 kb on - strand, within Pf1N1B4_50 at 57.074 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.087 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.221 kb on + strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.222 kb on - strand, within Pf1N1B4_51 at 57.409 kb on + strand at 57.432 kb on - strand at 57.504 kb on - strand at 57.512 kb on + strand at 57.512 kb on + strand at 57.513 kb on - strand at 57.603 kb on - strand at 57.603 kb on - strand at 57.717 kb on - strand at 57.717 kb on - strand at 57.875 kb on - strand, within Pf1N1B4_52 at 58.055 kb on - strand, within Pf1N1B4_52 at 58.072 kb on + strand, within Pf1N1B4_52 at 58.093 kb on + strand, within Pf1N1B4_52 at 58.142 kb on + strand at 58.182 kb on + strand, within Pf1N1B4_53 at 58.186 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.202 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.206 kb on + strand, within Pf1N1B4_53 at 58.207 kb on - strand, within Pf1N1B4_53 at 58.272 kb on + strand, within Pf1N1B4_53
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 56,516 + Pf1N1B4_49 0.38 -1.8 56,517 - Pf1N1B4_49 0.39 +2.1 56,520 - Pf1N1B4_49 0.40 +2.1 56,559 + Pf1N1B4_49 0.59 +1.3 56,559 + Pf1N1B4_49 0.59 +0.2 56,560 - Pf1N1B4_49 0.59 +0.3 56,560 - -0.8 56,560 - Pf1N1B4_49 0.59 -1.1 56,588 + Pf1N1B4_49 0.73 +1.2 56,588 + Pf1N1B4_49 0.73 -0.0 56,767 + -1.6 56,784 + -1.8 56,837 + Pf1N1B4_50 0.16 +1.1 56,837 + Pf1N1B4_50 0.16 -0.8 56,837 + Pf1N1B4_50 0.16 +0.8 56,837 + Pf1N1B4_50 0.16 -0.5 56,838 - Pf1N1B4_50 0.17 -1.1 56,838 - Pf1N1B4_50 0.17 -1.5 56,838 - Pf1N1B4_50 0.17 -0.6 56,838 - +1.3 56,895 + Pf1N1B4_50 0.48 +1.0 56,895 + -0.8 56,895 + Pf1N1B4_50 0.48 +0.1 56,895 + Pf1N1B4_50 0.48 +0.5 56,895 + Pf1N1B4_50 0.48 -0.0 56,895 + Pf1N1B4_50 0.48 -1.8 56,896 - +1.3 56,896 - Pf1N1B4_50 0.49 +0.2 56,896 - Pf1N1B4_50 0.49 +1.1 56,896 - Pf1N1B4_50 0.49 +1.3 56,896 - Pf1N1B4_50 0.49 +1.8 57,074 + +0.8 57,087 + +0.9 57,087 + -0.2 57,087 + -1.2 57,087 + +0.8 57,221 + Pf1N1B4_51 0.53 +1.2 57,221 + Pf1N1B4_51 0.53 -1.2 57,221 + +0.8 57,221 + Pf1N1B4_51 0.53 +0.9 57,221 + Pf1N1B4_51 0.53 -0.2 57,221 + Pf1N1B4_51 0.53 +0.6 57,221 + Pf1N1B4_51 0.53 +0.3 57,222 - Pf1N1B4_51 0.53 +0.9 57,222 - Pf1N1B4_51 0.53 +1.3 57,409 + +0.5 57,432 - -1.4 57,504 - +1.1 57,512 + +0.5 57,512 + -0.4 57,513 - -1.8 57,603 - +0.8 57,603 - -0.2 57,717 - +0.4 57,717 - +1.5 57,875 - Pf1N1B4_52 0.41 -1.2 58,055 - Pf1N1B4_52 0.80 -0.2 58,072 + Pf1N1B4_52 0.84 -0.8 58,093 + Pf1N1B4_52 0.89 -1.2 58,142 + -1.9 58,182 + Pf1N1B4_53 0.16 +0.8 58,186 + Pf1N1B4_53 0.18 -1.8 58,202 + Pf1N1B4_53 0.27 +0.1 58,202 + Pf1N1B4_53 0.27 +0.1 58,206 + -1.8 58,206 + Pf1N1B4_53 0.29 +0.8 58,206 + Pf1N1B4_53 0.29 +0.0 58,206 + Pf1N1B4_53 0.29 +0.4 58,206 + Pf1N1B4_53 0.29 +1.3 58,207 - Pf1N1B4_53 0.29 -0.2 58,272 + Pf1N1B4_53 0.63 -0.2
Or see this region's nucleotide sequence