Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4814 and Pf1N1B4_4815 overlap by 4 nucleotides Pf1N1B4_4815 and Pf1N1B4_4816 are separated by 63 nucleotides
Pf1N1B4_4814 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 5,316,451 to 5,317,512
_4814
Pf1N1B4_4815 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 5,317,509 to 5,318,693
_4815
Pf1N1B4_4816 - Ferrichrome-iron receptor, at 5,318,757 to 5,321,153
_4816
Position (kb)
5317
5318
5319 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5316.536 kb on - strand at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.103 kb on + strand, within Pf1N1B4_4814 at 5317.104 kb on - strand, within Pf1N1B4_4814 at 5317.104 kb on - strand at 5317.470 kb on - strand at 5317.492 kb on + strand at 5317.492 kb on + strand at 5317.493 kb on - strand at 5317.540 kb on + strand at 5317.666 kb on + strand, within Pf1N1B4_4815 at 5317.678 kb on + strand, within Pf1N1B4_4815 at 5318.201 kb on - strand, within Pf1N1B4_4815 at 5318.239 kb on + strand, within Pf1N1B4_4815 at 5318.482 kb on + strand, within Pf1N1B4_4815 at 5318.637 kb on + strand at 5318.839 kb on + strand at 5318.840 kb on - strand at 5318.971 kb on + strand at 5318.971 kb on + strand at 5318.986 kb on + strand at 5319.278 kb on + strand, within Pf1N1B4_4816 at 5319.295 kb on + strand, within Pf1N1B4_4816 at 5319.334 kb on + strand, within Pf1N1B4_4816 at 5319.334 kb on + strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.335 kb on - strand at 5319.335 kb on - strand, within Pf1N1B4_4816 at 5319.406 kb on + strand, within Pf1N1B4_4816 at 5319.406 kb on + strand, within Pf1N1B4_4816 at 5319.412 kb on + strand, within Pf1N1B4_4816 at 5319.511 kb on + strand at 5319.511 kb on + strand, within Pf1N1B4_4816 at 5319.511 kb on + strand, within Pf1N1B4_4816 at 5319.512 kb on - strand, within Pf1N1B4_4816 at 5319.512 kb on - strand at 5319.512 kb on - strand, within Pf1N1B4_4816 at 5319.676 kb on + strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816 at 5319.677 kb on - strand, within Pf1N1B4_4816
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,316,536 - -0.1 5,317,103 + Pf1N1B4_4814 0.61 +0.4 5,317,103 + Pf1N1B4_4814 0.61 +1.5 5,317,104 - Pf1N1B4_4814 0.61 +0.9 5,317,104 - -1.5 5,317,470 - -0.5 5,317,492 + +0.5 5,317,492 + +0.8 5,317,493 - +1.3 5,317,540 + -0.9 5,317,666 + Pf1N1B4_4815 0.13 -1.5 5,317,678 + Pf1N1B4_4815 0.14 -0.5 5,318,201 - Pf1N1B4_4815 0.58 -0.8 5,318,239 + Pf1N1B4_4815 0.62 -0.4 5,318,482 + Pf1N1B4_4815 0.82 -0.4 5,318,637 + -2.1 5,318,839 + +0.9 5,318,840 - +1.5 5,318,971 + +0.5 5,318,971 + +0.3 5,318,986 + +0.5 5,319,278 + Pf1N1B4_4816 0.22 -1.5 5,319,295 + Pf1N1B4_4816 0.22 +0.9 5,319,334 + Pf1N1B4_4816 0.24 +1.7 5,319,334 + Pf1N1B4_4816 0.24 +0.3 5,319,335 - Pf1N1B4_4816 0.24 -0.7 5,319,335 - Pf1N1B4_4816 0.24 -0.1 5,319,335 - Pf1N1B4_4816 0.24 +0.4 5,319,335 - Pf1N1B4_4816 0.24 -0.2 5,319,335 - +0.5 5,319,335 - Pf1N1B4_4816 0.24 -0.5 5,319,406 + Pf1N1B4_4816 0.27 +0.1 5,319,406 + Pf1N1B4_4816 0.27 -1.5 5,319,412 + Pf1N1B4_4816 0.27 -0.5 5,319,511 + +2.5 5,319,511 + Pf1N1B4_4816 0.31 -0.0 5,319,511 + Pf1N1B4_4816 0.31 -0.5 5,319,512 - Pf1N1B4_4816 0.31 +0.7 5,319,512 - -1.5 5,319,512 - Pf1N1B4_4816 0.31 +0.1 5,319,676 + Pf1N1B4_4816 0.38 +0.5 5,319,677 - Pf1N1B4_4816 0.38 -2.5 5,319,677 - Pf1N1B4_4816 0.38 +0.7 5,319,677 - Pf1N1B4_4816 0.38 +0.9 5,319,677 - Pf1N1B4_4816 0.38 -0.4 5,319,677 - Pf1N1B4_4816 0.38 +1.3
Or see this region's nucleotide sequence