Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4677 and Pf1N1B4_4678 are separated by 2 nucleotides Pf1N1B4_4678 and Pf1N1B4_4679 are separated by 132 nucleotides
Pf1N1B4_4677 - Trehalose synthase (EC 5.4.99.16), at 5,159,775 to 5,163,116
_4677
Pf1N1B4_4678 - 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18), at 5,163,119 to 5,165,350
_4678
Pf1N1B4_4679 - outer membrane autotransporter, at 5,165,483 to 5,166,550
_4679
Position (kb)
5163
5164
5165
5166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5162.323 kb on - strand, within Pf1N1B4_4677 at 5162.755 kb on - strand, within Pf1N1B4_4677 at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.756 kb on + strand, within Pf1N1B4_4677 at 5162.757 kb on - strand, within Pf1N1B4_4677 at 5162.757 kb on - strand, within Pf1N1B4_4677 at 5162.911 kb on - strand at 5162.927 kb on + strand at 5162.927 kb on + strand at 5163.064 kb on + strand at 5163.067 kb on - strand at 5163.114 kb on + strand at 5163.124 kb on + strand at 5163.124 kb on + strand at 5163.124 kb on + strand at 5163.444 kb on - strand, within Pf1N1B4_4678 at 5163.480 kb on - strand, within Pf1N1B4_4678 at 5163.716 kb on + strand, within Pf1N1B4_4678 at 5163.717 kb on - strand, within Pf1N1B4_4678 at 5163.896 kb on + strand, within Pf1N1B4_4678 at 5163.897 kb on - strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.995 kb on + strand, within Pf1N1B4_4678 at 5163.996 kb on - strand at 5163.996 kb on - strand, within Pf1N1B4_4678 at 5163.996 kb on - strand at 5163.996 kb on - strand, within Pf1N1B4_4678 at 5164.296 kb on - strand at 5164.325 kb on + strand at 5164.607 kb on + strand at 5164.609 kb on + strand, within Pf1N1B4_4678 at 5164.646 kb on + strand, within Pf1N1B4_4678 at 5164.647 kb on - strand at 5164.709 kb on + strand at 5164.709 kb on + strand, within Pf1N1B4_4678 at 5164.710 kb on - strand at 5164.710 kb on - strand, within Pf1N1B4_4678 at 5164.932 kb on - strand, within Pf1N1B4_4678 at 5164.932 kb on - strand, within Pf1N1B4_4678 at 5165.222 kb on + strand at 5165.353 kb on + strand at 5165.354 kb on - strand at 5165.553 kb on + strand at 5165.554 kb on - strand at 5165.568 kb on + strand at 5165.568 kb on + strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.569 kb on - strand at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.686 kb on - strand, within Pf1N1B4_4679 at 5165.716 kb on - strand, within Pf1N1B4_4679 at 5165.745 kb on + strand, within Pf1N1B4_4679 at 5165.746 kb on - strand at 5165.823 kb on + strand at 5165.824 kb on - strand, within Pf1N1B4_4679 at 5165.839 kb on + strand, within Pf1N1B4_4679 at 5165.839 kb on + strand, within Pf1N1B4_4679 at 5165.840 kb on - strand, within Pf1N1B4_4679 at 5165.884 kb on + strand, within Pf1N1B4_4679 at 5165.885 kb on - strand, within Pf1N1B4_4679 at 5165.887 kb on + strand, within Pf1N1B4_4679 at 5166.011 kb on + strand, within Pf1N1B4_4679 at 5166.331 kb on + strand, within Pf1N1B4_4679 at 5166.347 kb on + strand, within Pf1N1B4_4679 at 5166.347 kb on + strand at 5166.348 kb on - strand, within Pf1N1B4_4679
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,162,323 - Pf1N1B4_4677 0.76 +0.3 5,162,755 - Pf1N1B4_4677 0.89 +0.5 5,162,756 + Pf1N1B4_4677 0.89 -0.5 5,162,756 + -1.5 5,162,756 + Pf1N1B4_4677 0.89 +0.5 5,162,756 + -0.5 5,162,756 + Pf1N1B4_4677 0.89 -3.3 5,162,756 + Pf1N1B4_4677 0.89 -0.0 5,162,757 - Pf1N1B4_4677 0.89 +0.3 5,162,757 - Pf1N1B4_4677 0.89 -0.5 5,162,911 - -0.8 5,162,927 + +0.9 5,162,927 + +0.6 5,163,064 + +0.2 5,163,067 - +0.1 5,163,114 + -0.9 5,163,124 + -2.5 5,163,124 + +1.2 5,163,124 + +0.5 5,163,444 - Pf1N1B4_4678 0.15 +0.2 5,163,480 - Pf1N1B4_4678 0.16 -1.5 5,163,716 + Pf1N1B4_4678 0.27 +1.1 5,163,717 - Pf1N1B4_4678 0.27 +0.8 5,163,896 + Pf1N1B4_4678 0.35 -0.7 5,163,897 - Pf1N1B4_4678 0.35 -2.9 5,163,995 + Pf1N1B4_4678 0.39 -0.8 5,163,995 + Pf1N1B4_4678 0.39 -1.5 5,163,995 + Pf1N1B4_4678 0.39 +0.5 5,163,995 + Pf1N1B4_4678 0.39 -0.5 5,163,996 - +1.1 5,163,996 - Pf1N1B4_4678 0.39 -0.0 5,163,996 - +0.5 5,163,996 - Pf1N1B4_4678 0.39 -2.5 5,164,296 - -0.5 5,164,325 + -0.2 5,164,607 + -0.5 5,164,609 + Pf1N1B4_4678 0.67 +0.5 5,164,646 + Pf1N1B4_4678 0.68 +1.8 5,164,647 - +1.8 5,164,709 + +1.1 5,164,709 + Pf1N1B4_4678 0.71 -1.5 5,164,710 - -1.5 5,164,710 - Pf1N1B4_4678 0.71 -1.1 5,164,932 - Pf1N1B4_4678 0.81 -1.5 5,164,932 - Pf1N1B4_4678 0.81 +0.0 5,165,222 + -1.5 5,165,353 + +0.3 5,165,354 - -0.5 5,165,553 + -2.1 5,165,554 - +0.5 5,165,568 + +1.8 5,165,568 + +0.1 5,165,569 - -0.5 5,165,569 - -0.5 5,165,569 - +1.7 5,165,569 - +1.0 5,165,569 - +0.5 5,165,686 - Pf1N1B4_4679 0.19 -0.9 5,165,686 - Pf1N1B4_4679 0.19 -3.1 5,165,686 - Pf1N1B4_4679 0.19 -0.1 5,165,716 - Pf1N1B4_4679 0.22 +0.7 5,165,745 + Pf1N1B4_4679 0.25 +1.2 5,165,746 - +0.5 5,165,823 + -1.1 5,165,824 - Pf1N1B4_4679 0.32 +0.2 5,165,839 + Pf1N1B4_4679 0.33 -0.2 5,165,839 + Pf1N1B4_4679 0.33 +1.1 5,165,840 - Pf1N1B4_4679 0.33 -0.9 5,165,884 + Pf1N1B4_4679 0.38 -1.5 5,165,885 - Pf1N1B4_4679 0.38 -0.3 5,165,887 + Pf1N1B4_4679 0.38 +0.5 5,166,011 + Pf1N1B4_4679 0.49 +1.1 5,166,331 + Pf1N1B4_4679 0.79 -0.2 5,166,347 + Pf1N1B4_4679 0.81 +0.1 5,166,347 + -0.2 5,166,348 - Pf1N1B4_4679 0.81 +0.5
Or see this region's nucleotide sequence