Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4521 and Pf1N1B4_4522 are separated by 140 nucleotides Pf1N1B4_4522 and Pf1N1B4_4523 are separated by 3 nucleotides Pf1N1B4_4523 and Pf1N1B4_4524 are separated by 212 nucleotides
Pf1N1B4_4521 - Probable transmembrane protein, at 4,985,051 to 4,985,485
_4521
Pf1N1B4_4522 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 4,985,626 to 4,986,060
_4522
Pf1N1B4_4523 - RNA polymerase sigma-70 factor, ECF subfamily, at 4,986,064 to 4,986,957
_4523
Pf1N1B4_4524 - Sensory box transcriptional regulator, LuxR family, at 4,987,170 to 4,988,666
_4524
Position (kb) 4986
4987 Strain fitness (log2 ratio) -1
0
1
2 at 4985.265 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.269 kb on - strand, within Pf1N1B4_4521 at 4985.300 kb on + strand, within Pf1N1B4_4521 at 4985.301 kb on - strand, within Pf1N1B4_4521 at 4985.518 kb on - strand at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4985.966 kb on + strand, within Pf1N1B4_4522 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.285 kb on + strand, within Pf1N1B4_4523 at 4986.301 kb on + strand, within Pf1N1B4_4523 at 4986.777 kb on + strand, within Pf1N1B4_4523 at 4986.778 kb on - strand, within Pf1N1B4_4523 at 4986.778 kb on - strand, within Pf1N1B4_4523 at 4986.956 kb on - strand at 4987.098 kb on + strand at 4987.408 kb on - strand, within Pf1N1B4_4524 at 4987.450 kb on + strand, within Pf1N1B4_4524 at 4987.451 kb on - strand at 4987.451 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,985,265 - Pf1N1B4_4521 0.49 -0.5 4,985,269 - -0.9 4,985,269 - Pf1N1B4_4521 0.50 +0.3 4,985,269 - Pf1N1B4_4521 0.50 +1.3 4,985,269 - Pf1N1B4_4521 0.50 +0.0 4,985,300 + Pf1N1B4_4521 0.57 +0.3 4,985,301 - Pf1N1B4_4521 0.57 -0.2 4,985,518 - +1.1 4,985,966 + Pf1N1B4_4522 0.78 +0.9 4,985,966 + Pf1N1B4_4522 0.78 +0.9 4,986,285 + Pf1N1B4_4523 0.25 +0.7 4,986,285 + Pf1N1B4_4523 0.25 +1.1 4,986,301 + Pf1N1B4_4523 0.27 +0.5 4,986,777 + Pf1N1B4_4523 0.80 +0.5 4,986,778 - Pf1N1B4_4523 0.80 +1.0 4,986,778 - Pf1N1B4_4523 0.80 +0.3 4,986,956 - +2.7 4,987,098 + +0.9 4,987,408 - Pf1N1B4_4524 0.16 +0.4 4,987,450 + Pf1N1B4_4524 0.19 -0.9 4,987,451 - +2.1 4,987,451 - +0.9
Or see this region's nucleotide sequence