Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4516 and Pf1N1B4_4517 are separated by 160 nucleotides Pf1N1B4_4517 and Pf1N1B4_4518 are separated by 622 nucleotides
Pf1N1B4_4516 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 4,978,107 to 4,979,474
_4516
Pf1N1B4_4517 - Dihydropyrimidinase (EC 3.5.2.2), at 4,979,635 to 4,981,074
_4517
Pf1N1B4_4518 - Possible pyrimidine permease in reductive pathway, at 4,981,697 to 4,983,181
_4518
Position (kb)
4979
4980
4981
4982 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4978.702 kb on + strand, within Pf1N1B4_4516 at 4978.703 kb on - strand at 4979.493 kb on - strand at 4979.658 kb on + strand at 4979.659 kb on - strand at 4979.659 kb on - strand at 4979.721 kb on - strand at 4980.296 kb on + strand at 4980.369 kb on - strand, within Pf1N1B4_4517 at 4980.716 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.892 kb on - strand at 4981.010 kb on + strand at 4981.025 kb on + strand at 4981.042 kb on - strand at 4981.115 kb on + strand at 4981.125 kb on - strand at 4981.149 kb on + strand at 4981.782 kb on - strand at 4981.880 kb on + strand, within Pf1N1B4_4518 at 4981.881 kb on - strand, within Pf1N1B4_4518 at 4982.065 kb on - strand, within Pf1N1B4_4518
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,978,702 + Pf1N1B4_4516 0.43 -0.6 4,978,703 - -2.0 4,979,493 - -0.5 4,979,658 + -1.1 4,979,659 - +0.4 4,979,659 - +1.4 4,979,721 - +0.5 4,980,296 + +2.1 4,980,369 - Pf1N1B4_4517 0.51 -0.9 4,980,716 + Pf1N1B4_4517 0.75 +1.5 4,980,891 + Pf1N1B4_4517 0.87 +0.5 4,980,891 + Pf1N1B4_4517 0.87 -1.5 4,980,891 + Pf1N1B4_4517 0.87 -1.2 4,980,891 + Pf1N1B4_4517 0.87 -0.5 4,980,892 - -0.5 4,981,010 + +0.1 4,981,025 + +0.0 4,981,042 - +1.1 4,981,115 + -0.2 4,981,125 - -0.8 4,981,149 + +0.0 4,981,782 - -0.5 4,981,880 + Pf1N1B4_4518 0.12 -1.1 4,981,881 - Pf1N1B4_4518 0.12 -0.5 4,982,065 - Pf1N1B4_4518 0.25 -0.7
Or see this region's nucleotide sequence