Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4477 and Pf1N1B4_4478 are separated by 18 nucleotides Pf1N1B4_4478 and Pf1N1B4_4479 are separated by 0 nucleotides
Pf1N1B4_4477 - Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4), at 4,937,778 to 4,939,160
_4477
Pf1N1B4_4478 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 4,939,179 to 4,940,450
_4478
Pf1N1B4_4479 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 4,940,451 to 4,941,509
_4479
Position (kb)
4939
4940
4941 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4938.254 kb on - strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.431 kb on + strand, within Pf1N1B4_4477 at 4938.432 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.533 kb on - strand, within Pf1N1B4_4477 at 4938.619 kb on + strand, within Pf1N1B4_4477 at 4939.108 kb on + strand at 4939.181 kb on - strand at 4939.787 kb on - strand at 4939.933 kb on + strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4939.934 kb on - strand, within Pf1N1B4_4478 at 4940.195 kb on + strand, within Pf1N1B4_4478 at 4940.432 kb on + strand at 4940.432 kb on + strand at 4940.433 kb on - strand at 4940.506 kb on + strand at 4940.575 kb on + strand, within Pf1N1B4_4479 at 4940.773 kb on + strand, within Pf1N1B4_4479 at 4940.774 kb on - strand at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.774 kb on - strand, within Pf1N1B4_4479 at 4940.788 kb on + strand, within Pf1N1B4_4479 at 4940.789 kb on - strand, within Pf1N1B4_4479 at 4940.851 kb on + strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.852 kb on - strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4940.935 kb on + strand, within Pf1N1B4_4479 at 4941.010 kb on + strand, within Pf1N1B4_4479 at 4941.095 kb on - strand, within Pf1N1B4_4479 at 4941.209 kb on - strand, within Pf1N1B4_4479 at 4941.323 kb on - strand, within Pf1N1B4_4479 at 4941.367 kb on + strand, within Pf1N1B4_4479
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,938,254 - Pf1N1B4_4477 0.34 -0.9 4,938,431 + Pf1N1B4_4477 0.47 -0.7 4,938,431 + Pf1N1B4_4477 0.47 +0.6 4,938,432 - Pf1N1B4_4477 0.47 -0.4 4,938,533 - Pf1N1B4_4477 0.55 +1.1 4,938,533 - Pf1N1B4_4477 0.55 -0.4 4,938,619 + Pf1N1B4_4477 0.61 +0.6 4,939,108 + +0.1 4,939,181 - -1.4 4,939,787 - -1.4 4,939,933 + Pf1N1B4_4478 0.59 -1.0 4,939,934 - Pf1N1B4_4478 0.59 +0.6 4,939,934 - Pf1N1B4_4478 0.59 +2.3 4,940,195 + Pf1N1B4_4478 0.80 +2.1 4,940,432 + +1.0 4,940,432 + +0.6 4,940,433 - +0.6 4,940,506 + -0.0 4,940,575 + Pf1N1B4_4479 0.12 -1.0 4,940,773 + Pf1N1B4_4479 0.30 -1.4 4,940,774 - +1.1 4,940,774 - Pf1N1B4_4479 0.31 +0.9 4,940,774 - Pf1N1B4_4479 0.31 -2.0 4,940,788 + Pf1N1B4_4479 0.32 -0.4 4,940,789 - Pf1N1B4_4479 0.32 -0.4 4,940,851 + Pf1N1B4_4479 0.38 -0.0 4,940,852 - Pf1N1B4_4479 0.38 -0.4 4,940,852 - Pf1N1B4_4479 0.38 +0.9 4,940,935 + Pf1N1B4_4479 0.46 +1.9 4,940,935 + Pf1N1B4_4479 0.46 +0.6 4,941,010 + Pf1N1B4_4479 0.53 +1.7 4,941,095 - Pf1N1B4_4479 0.61 -1.4 4,941,209 - Pf1N1B4_4479 0.72 -1.2 4,941,323 - Pf1N1B4_4479 0.82 -1.4 4,941,367 + Pf1N1B4_4479 0.86 +0.6
Or see this region's nucleotide sequence