Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4311 and Pf1N1B4_4312 are separated by 215 nucleotides Pf1N1B4_4312 and Pf1N1B4_4313 are separated by 173 nucleotides Pf1N1B4_4313 and Pf1N1B4_4314 are separated by 233 nucleotides
Pf1N1B4_4311 - Phosphatidylcholine synthase (EC 2.7.8.24), at 4,772,060 to 4,772,782
_4311
Pf1N1B4_4312 - FIG00792904: hypothetical protein, at 4,772,998 to 4,773,369
_4312
Pf1N1B4_4313 - putative membrane protein, at 4,773,543 to 4,774,268
_4313
Pf1N1B4_4314 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 4,774,502 to 4,777,126
_4314
Position (kb)
4773
4774
4775 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4772.769 kb on - strand at 4772.884 kb on + strand at 4772.884 kb on + strand at 4772.885 kb on - strand at 4772.885 kb on - strand at 4772.898 kb on + strand at 4772.898 kb on + strand at 4772.898 kb on + strand at 4772.898 kb on + strand at 4772.899 kb on - strand at 4772.899 kb on - strand at 4772.899 kb on - strand at 4772.954 kb on - strand at 4772.963 kb on + strand at 4772.964 kb on - strand at 4772.964 kb on - strand at 4773.309 kb on - strand, within Pf1N1B4_4312 at 4773.341 kb on + strand at 4773.400 kb on + strand at 4773.401 kb on - strand at 4773.401 kb on - strand at 4773.571 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.039 kb on + strand at 4774.039 kb on + strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand, within Pf1N1B4_4313 at 4774.040 kb on - strand at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.131 kb on + strand, within Pf1N1B4_4313 at 4774.132 kb on - strand at 4774.132 kb on - strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.182 kb on + strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.183 kb on - strand, within Pf1N1B4_4313 at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.231 kb on + strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.232 kb on - strand at 4774.281 kb on + strand at 4774.293 kb on + strand at 4774.293 kb on + strand at 4774.360 kb on + strand at 4774.361 kb on - strand at 4774.362 kb on + strand at 4774.362 kb on + strand at 4774.363 kb on - strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.462 kb on + strand at 4774.463 kb on - strand at 4774.523 kb on + strand at 4774.649 kb on + strand at 4775.016 kb on - strand, within Pf1N1B4_4314 at 4775.237 kb on + strand, within Pf1N1B4_4314 at 4775.238 kb on - strand, within Pf1N1B4_4314
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,772,769 - -1.0 4,772,884 + +0.6 4,772,884 + -1.2 4,772,885 - -0.4 4,772,885 - +1.6 4,772,898 + +0.4 4,772,898 + +1.6 4,772,898 + +0.6 4,772,898 + -0.7 4,772,899 - +0.2 4,772,899 - -2.2 4,772,899 - -0.4 4,772,954 - -0.2 4,772,963 + +0.4 4,772,964 - -1.4 4,772,964 - -1.0 4,773,309 - Pf1N1B4_4312 0.84 +0.6 4,773,341 + +1.9 4,773,400 + +2.6 4,773,401 - +0.7 4,773,401 - +0.4 4,773,571 + -0.4 4,774,039 + Pf1N1B4_4313 0.68 -0.4 4,774,039 + -0.4 4,774,039 + Pf1N1B4_4313 0.68 +1.6 4,774,040 - Pf1N1B4_4313 0.68 +1.4 4,774,040 - Pf1N1B4_4313 0.68 -0.8 4,774,040 - Pf1N1B4_4313 0.68 +1.2 4,774,040 - +2.2 4,774,131 + Pf1N1B4_4313 0.81 +1.2 4,774,131 + Pf1N1B4_4313 0.81 +1.5 4,774,131 + Pf1N1B4_4313 0.81 -0.4 4,774,132 - +1.9 4,774,132 - Pf1N1B4_4313 0.81 -0.8 4,774,182 + Pf1N1B4_4313 0.88 -2.4 4,774,182 + Pf1N1B4_4313 0.88 +0.9 4,774,183 - Pf1N1B4_4313 0.88 +0.2 4,774,183 - Pf1N1B4_4313 0.88 -2.4 4,774,183 - Pf1N1B4_4313 0.88 +0.3 4,774,231 + -0.2 4,774,231 + +0.6 4,774,231 + +1.9 4,774,232 - -0.7 4,774,232 - -0.4 4,774,232 - -1.0 4,774,232 - +0.6 4,774,281 + +1.3 4,774,293 + +0.4 4,774,293 + +0.6 4,774,360 + -0.1 4,774,361 - -0.4 4,774,362 + -0.7 4,774,362 + +0.1 4,774,363 - +0.7 4,774,462 + -0.0 4,774,462 + -1.4 4,774,462 + +0.6 4,774,462 + -0.0 4,774,462 + +0.3 4,774,463 - +0.7 4,774,523 + -0.0 4,774,649 + +1.2 4,775,016 - Pf1N1B4_4314 0.20 -0.0 4,775,237 + Pf1N1B4_4314 0.28 -0.7 4,775,238 - Pf1N1B4_4314 0.28 -0.0
Or see this region's nucleotide sequence