Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4274 and Pf1N1B4_4275 are separated by 172 nucleotides Pf1N1B4_4275 and Pf1N1B4_4276 overlap by 4 nucleotides Pf1N1B4_4276 and Pf1N1B4_4277 are separated by 44 nucleotides
Pf1N1B4_4274 - 5-keto-2-deoxygluconokinase (EC 2.7.1.92) / uncharacterized domain, at 4,738,398 to 4,740,335
_4274
Pf1N1B4_4275 - Inosose dehydratase (EC 4.2.1.44), at 4,740,508 to 4,741,401
_4275
Pf1N1B4_4276 - 5-deoxy-glucuronate isomerase (EC 5.3.1.-), at 4,741,398 to 4,742,207
_4276
Pf1N1B4_4277 - Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27), at 4,742,252 to 4,743,754
_4277
Position (kb)
4740
4741
4742 Strain fitness (log2 ratio)
-2
-1
0
1 at 4739.898 kb on + strand, within Pf1N1B4_4274 at 4739.899 kb on - strand, within Pf1N1B4_4274 at 4740.339 kb on - strand at 4740.350 kb on - strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.411 kb on + strand at 4740.425 kb on - strand at 4740.494 kb on + strand at 4740.691 kb on + strand, within Pf1N1B4_4275 at 4740.786 kb on - strand, within Pf1N1B4_4275 at 4740.965 kb on - strand, within Pf1N1B4_4275 at 4741.069 kb on + strand, within Pf1N1B4_4275 at 4741.069 kb on + strand, within Pf1N1B4_4275 at 4741.070 kb on - strand, within Pf1N1B4_4275 at 4741.070 kb on - strand at 4741.070 kb on - strand, within Pf1N1B4_4275 at 4741.336 kb on + strand at 4741.467 kb on + strand at 4741.468 kb on - strand at 4741.468 kb on - strand at 4741.662 kb on + strand, within Pf1N1B4_4276 at 4741.822 kb on - strand at 4741.908 kb on + strand, within Pf1N1B4_4276 at 4741.968 kb on + strand, within Pf1N1B4_4276 at 4741.968 kb on + strand, within Pf1N1B4_4276 at 4741.968 kb on + strand, within Pf1N1B4_4276 at 4741.968 kb on + strand, within Pf1N1B4_4276 at 4741.968 kb on + strand, within Pf1N1B4_4276 at 4741.969 kb on - strand, within Pf1N1B4_4276 at 4741.969 kb on - strand at 4741.971 kb on + strand, within Pf1N1B4_4276 at 4742.124 kb on + strand, within Pf1N1B4_4276 at 4742.124 kb on + strand, within Pf1N1B4_4276 at 4742.125 kb on - strand, within Pf1N1B4_4276 at 4742.127 kb on + strand at 4742.280 kb on - strand at 4742.295 kb on + strand at 4742.295 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,739,898 + Pf1N1B4_4274 0.77 -0.4 4,739,899 - Pf1N1B4_4274 0.77 +0.6 4,740,339 - -0.7 4,740,350 - +0.3 4,740,411 + +0.2 4,740,411 + -0.0 4,740,411 + -0.4 4,740,411 + +0.2 4,740,411 + -2.4 4,740,425 - -0.4 4,740,494 + -1.7 4,740,691 + Pf1N1B4_4275 0.20 -1.4 4,740,786 - Pf1N1B4_4275 0.31 -1.4 4,740,965 - Pf1N1B4_4275 0.51 +0.1 4,741,069 + Pf1N1B4_4275 0.63 -0.4 4,741,069 + Pf1N1B4_4275 0.63 -1.7 4,741,070 - Pf1N1B4_4275 0.63 -0.4 4,741,070 - -0.4 4,741,070 - Pf1N1B4_4275 0.63 -0.4 4,741,336 + +0.6 4,741,467 + -1.6 4,741,468 - +0.4 4,741,468 - +1.2 4,741,662 + Pf1N1B4_4276 0.33 -0.2 4,741,822 - +0.6 4,741,908 + Pf1N1B4_4276 0.63 -0.4 4,741,968 + Pf1N1B4_4276 0.70 +0.6 4,741,968 + Pf1N1B4_4276 0.70 -2.7 4,741,968 + Pf1N1B4_4276 0.70 +0.6 4,741,968 + Pf1N1B4_4276 0.70 -1.2 4,741,968 + Pf1N1B4_4276 0.70 -0.7 4,741,969 - Pf1N1B4_4276 0.70 -1.4 4,741,969 - +0.2 4,741,971 + Pf1N1B4_4276 0.71 -1.1 4,742,124 + Pf1N1B4_4276 0.90 +0.2 4,742,124 + Pf1N1B4_4276 0.90 +1.0 4,742,125 - Pf1N1B4_4276 0.90 +0.2 4,742,127 + -1.0 4,742,280 - +0.9 4,742,295 + -0.0 4,742,295 + +0.6
Or see this region's nucleotide sequence