Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4002 and Pf1N1B4_4003 are separated by 126 nucleotides Pf1N1B4_4003 and Pf1N1B4_4004 overlap by 4 nucleotides Pf1N1B4_4004 and Pf1N1B4_4005 are separated by 262 nucleotides
Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404
_4002
Pf1N1B4_4003 - UDP-2,3-diacylglucosamine diphosphatase (EC 3.6.1.54), at 4,452,531 to 4,453,283
_4003
Pf1N1B4_4004 - Peptidyl-prolyl cis-trans isomerase PpiB (EC 5.2.1.8), at 4,453,280 to 4,453,783
_4004
Pf1N1B4_4005 - Glutaminyl-tRNA synthetase (EC 6.1.1.18), at 4,454,046 to 4,455,743
_4005
Position (kb)
4453
4454 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4452.300 kb on + strand at 4452.301 kb on - strand at 4453.326 kb on - strand at 4453.410 kb on - strand, within Pf1N1B4_4004 at 4453.417 kb on - strand, within Pf1N1B4_4004 at 4453.534 kb on - strand, within Pf1N1B4_4004 at 4453.800 kb on - strand at 4453.825 kb on - strand at 4453.870 kb on + strand at 4453.918 kb on + strand at 4453.919 kb on - strand at 4453.974 kb on + strand at 4454.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,452,300 + +1.2 4,452,301 - +0.3 4,453,326 - -0.2 4,453,410 - Pf1N1B4_4004 0.26 +0.6 4,453,417 - Pf1N1B4_4004 0.27 +0.3 4,453,534 - Pf1N1B4_4004 0.50 +1.0 4,453,800 - -1.8 4,453,825 - -1.8 4,453,870 + +1.8 4,453,918 + -0.2 4,453,919 - -1.0 4,453,974 + -0.1 4,454,030 + -2.1
Or see this region's nucleotide sequence