Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3799 and Pf1N1B4_3800 are separated by 214 nucleotides Pf1N1B4_3800 and Pf1N1B4_5229 are separated by 308 nucleotides Pf1N1B4_5229 and Pf1N1B4_3801 are separated by 321 nucleotides Pf1N1B4_3801 and Pf1N1B4_3802 are separated by 68 nucleotides
Pf1N1B4_3799 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 4,208,246 to 4,208,968
_3799
Pf1N1B4_3800 - Enoyl-CoA hydratase/isomerase family protein, at 4,209,183 to 4,209,872
_3800
Pf1N1B4_5229 - tRNA-Ser-GGA, at 4,210,181 to 4,210,267
_5229
Pf1N1B4_3801 - hypothetical protein, at 4,210,589 to 4,210,786
_3801
Pf1N1B4_3802 - PlcB, ORFX, ORFP, ORFB, ORFA, ldh gene, at 4,210,855 to 4,211,175
_3802
Position (kb)
4209
4210 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4208.202 kb on + strand at 4208.258 kb on + strand at 4208.258 kb on + strand at 4208.258 kb on + strand at 4208.259 kb on - strand at 4208.259 kb on - strand at 4208.421 kb on + strand, within Pf1N1B4_3799 at 4208.422 kb on - strand, within Pf1N1B4_3799 at 4208.422 kb on - strand, within Pf1N1B4_3799 at 4208.737 kb on - strand, within Pf1N1B4_3799 at 4208.779 kb on + strand, within Pf1N1B4_3799 at 4208.844 kb on + strand, within Pf1N1B4_3799 at 4208.845 kb on - strand, within Pf1N1B4_3799 at 4208.845 kb on - strand, within Pf1N1B4_3799 at 4208.847 kb on - strand, within Pf1N1B4_3799 at 4208.859 kb on - strand, within Pf1N1B4_3799 at 4208.927 kb on - strand at 4208.950 kb on + strand at 4209.038 kb on + strand at 4209.038 kb on + strand at 4209.081 kb on + strand at 4209.081 kb on + strand at 4209.111 kb on - strand at 4209.185 kb on - strand at 4209.191 kb on + strand at 4209.192 kb on - strand at 4209.192 kb on - strand at 4209.267 kb on - strand at 4209.548 kb on + strand, within Pf1N1B4_3800 at 4209.549 kb on - strand, within Pf1N1B4_3800 at 4209.692 kb on - strand, within Pf1N1B4_3800 at 4209.722 kb on + strand, within Pf1N1B4_3800 at 4209.723 kb on - strand, within Pf1N1B4_3800 at 4209.854 kb on - strand at 4209.888 kb on - strand at 4209.888 kb on - strand at 4209.890 kb on + strand at 4209.891 kb on - strand at 4209.891 kb on - strand at 4209.928 kb on + strand at 4209.928 kb on + strand at 4210.010 kb on + strand at 4210.010 kb on + strand at 4210.010 kb on + strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.011 kb on - strand at 4210.013 kb on - strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.014 kb on + strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.015 kb on - strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.370 kb on + strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.371 kb on - strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.399 kb on + strand at 4210.400 kb on - strand at 4210.400 kb on - strand at 4210.426 kb on + strand at 4210.426 kb on + strand at 4210.426 kb on + strand at 4210.427 kb on - strand at 4210.427 kb on - strand at 4210.499 kb on + strand at 4210.499 kb on + strand at 4210.500 kb on - strand at 4210.588 kb on + strand at 4210.729 kb on + strand, within Pf1N1B4_3801 at 4210.730 kb on - strand, within Pf1N1B4_3801 at 4210.744 kb on + strand, within Pf1N1B4_3801 at 4210.745 kb on - strand, within Pf1N1B4_3801 at 4210.784 kb on + strand at 4210.785 kb on - strand at 4210.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,208,202 + +0.4 4,208,258 + +1.4 4,208,258 + -0.9 4,208,258 + -0.1 4,208,259 - -1.2 4,208,259 - +0.0 4,208,421 + Pf1N1B4_3799 0.24 -0.4 4,208,422 - Pf1N1B4_3799 0.24 -0.2 4,208,422 - Pf1N1B4_3799 0.24 -0.9 4,208,737 - Pf1N1B4_3799 0.68 -1.5 4,208,779 + Pf1N1B4_3799 0.74 -2.2 4,208,844 + Pf1N1B4_3799 0.83 -1.2 4,208,845 - Pf1N1B4_3799 0.83 +1.4 4,208,845 - Pf1N1B4_3799 0.83 +1.0 4,208,847 - Pf1N1B4_3799 0.83 -0.9 4,208,859 - Pf1N1B4_3799 0.85 +2.1 4,208,927 - +0.1 4,208,950 + +0.1 4,209,038 + +2.3 4,209,038 + -0.0 4,209,081 + -0.4 4,209,081 + -0.6 4,209,111 - +1.4 4,209,185 - -1.2 4,209,191 + -0.4 4,209,192 - +2.6 4,209,192 - -0.0 4,209,267 - +2.4 4,209,548 + Pf1N1B4_3800 0.53 +0.2 4,209,549 - Pf1N1B4_3800 0.53 -1.2 4,209,692 - Pf1N1B4_3800 0.74 -0.6 4,209,722 + Pf1N1B4_3800 0.78 +0.4 4,209,723 - Pf1N1B4_3800 0.78 -1.0 4,209,854 - +1.8 4,209,888 - -1.0 4,209,888 - -0.2 4,209,890 + -0.4 4,209,891 - -0.2 4,209,891 - -0.2 4,209,928 + -0.2 4,209,928 + -0.8 4,210,010 + +3.0 4,210,010 + +1.6 4,210,010 + -0.5 4,210,011 - -2.1 4,210,011 - +1.8 4,210,011 - -1.4 4,210,011 - -1.2 4,210,011 - +0.1 4,210,011 - -0.5 4,210,013 - -0.1 4,210,014 + +0.8 4,210,014 + +1.8 4,210,014 + -1.8 4,210,014 + +2.1 4,210,014 + +0.8 4,210,014 + +1.1 4,210,014 + +2.1 4,210,015 - -0.5 4,210,015 - +1.8 4,210,015 - +0.3 4,210,015 - -0.2 4,210,015 - +0.5 4,210,015 - +0.8 4,210,015 - -1.8 4,210,015 - +0.1 4,210,015 - +0.1 4,210,015 - +0.6 4,210,370 + -0.5 4,210,370 + -0.6 4,210,370 + +0.5 4,210,370 + +0.4 4,210,371 - -1.8 4,210,371 - -0.2 4,210,371 - +0.8 4,210,371 - -0.2 4,210,371 - +0.8 4,210,399 + -1.2 4,210,399 + -0.2 4,210,399 + +2.8 4,210,399 + +0.2 4,210,399 + -0.9 4,210,399 + -0.2 4,210,400 - -0.8 4,210,400 - -1.2 4,210,426 + +1.4 4,210,426 + +2.1 4,210,426 + +0.8 4,210,427 - +1.0 4,210,427 - +1.1 4,210,499 + +2.4 4,210,499 + -0.2 4,210,500 - +1.6 4,210,588 + +2.1 4,210,729 + Pf1N1B4_3801 0.71 -2.2 4,210,730 - Pf1N1B4_3801 0.71 -1.4 4,210,744 + Pf1N1B4_3801 0.78 +0.8 4,210,745 - Pf1N1B4_3801 0.79 -1.4 4,210,784 + -0.2 4,210,785 - -0.9 4,210,838 - -0.2
Or see this region's nucleotide sequence