Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3773 and Pf1N1B4_3774 are separated by 77 nucleotides Pf1N1B4_3774 and Pf1N1B4_3775 are separated by 75 nucleotides Pf1N1B4_3775 and Pf1N1B4_3776 are separated by 131 nucleotides Pf1N1B4_3776 and Pf1N1B4_3777 are separated by 238 nucleotides
Pf1N1B4_3773 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 4,183,521 to 4,184,066
_3773
Pf1N1B4_3774 - Smr domain protein, at 4,184,144 to 4,184,701
_3774
Pf1N1B4_3775 - FIG00956876: hypothetical protein, at 4,184,777 to 4,185,097
_3775
Pf1N1B4_3776 - Isochorismatase (EC 3.3.2.1), at 4,185,229 to 4,185,819
_3776
Pf1N1B4_3777 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 4,186,058 to 4,186,966
_3777
Position (kb)
4184
4185
4186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4184.187 kb on + strand at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.331 kb on - strand, within Pf1N1B4_3774 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4184.872 kb on - strand, within Pf1N1B4_3775 at 4185.893 kb on + strand at 4185.893 kb on + strand at 4185.894 kb on - strand at 4185.947 kb on - strand at 4185.947 kb on - strand at 4185.963 kb on - strand at 4185.973 kb on + strand at 4185.973 kb on + strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4185.974 kb on - strand at 4186.023 kb on + strand at 4186.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,184,187 + -0.2 4,184,331 - Pf1N1B4_3774 0.34 +1.7 4,184,331 - Pf1N1B4_3774 0.34 +1.8 4,184,872 - Pf1N1B4_3775 0.30 -2.2 4,184,872 - Pf1N1B4_3775 0.30 -0.1 4,184,872 - Pf1N1B4_3775 0.30 -2.6 4,185,893 + +0.8 4,185,893 + +0.6 4,185,894 - +0.5 4,185,947 - +0.1 4,185,947 - -0.9 4,185,963 - +0.8 4,185,973 + +1.4 4,185,973 + +1.6 4,185,974 - +0.1 4,185,974 - -3.3 4,185,974 - -1.4 4,186,023 + -1.2 4,186,094 - -2.5
Or see this region's nucleotide sequence