Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3589 and Pf1N1B4_3590 are separated by 155 nucleotides Pf1N1B4_3590 and Pf1N1B4_3591 overlap by 18 nucleotides Pf1N1B4_3591 and Pf1N1B4_3592 are separated by 83 nucleotides
Pf1N1B4_3589 - N-acetylneuraminate synthase (EC 2.5.1.56), at 4,001,835 to 4,002,887
_3589
Pf1N1B4_3590 - hypothetical protein, at 4,003,043 to 4,003,180
_3590
Pf1N1B4_3591 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41), at 4,003,163 to 4,004,044
_3591
Pf1N1B4_3592 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 4,004,128 to 4,005,420
_3592
Position (kb)
4003
4004 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4002.225 kb on + strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.226 kb on - strand, within Pf1N1B4_3589 at 4002.256 kb on + strand, within Pf1N1B4_3589 at 4002.257 kb on - strand, within Pf1N1B4_3589 at 4002.402 kb on + strand, within Pf1N1B4_3589 at 4002.704 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.721 kb on - strand, within Pf1N1B4_3589 at 4002.915 kb on - strand at 4002.934 kb on - strand at 4002.934 kb on - strand at 4002.951 kb on + strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.952 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.957 kb on - strand at 4002.959 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.009 kb on - strand at 4003.045 kb on - strand at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.089 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.101 kb on - strand, within Pf1N1B4_3590 at 4003.128 kb on + strand, within Pf1N1B4_3590 at 4003.129 kb on - strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.145 kb on + strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.146 kb on - strand, within Pf1N1B4_3590 at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.172 kb on + strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.173 kb on - strand at 4003.444 kb on + strand, within Pf1N1B4_3591 at 4003.445 kb on - strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.466 kb on + strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.467 kb on - strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.481 kb on + strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.482 kb on - strand, within Pf1N1B4_3591 at 4003.547 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4003.918 kb on + strand, within Pf1N1B4_3591 at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.033 kb on + strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.034 kb on - strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.050 kb on + strand at 4004.051 kb on - strand at 4004.051 kb on - strand at 4004.083 kb on + strand at 4004.097 kb on - strand at 4004.163 kb on - strand at 4004.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,002,225 + Pf1N1B4_3589 0.37 +2.6 4,002,226 - Pf1N1B4_3589 0.37 +0.2 4,002,226 - Pf1N1B4_3589 0.37 -0.3 4,002,226 - Pf1N1B4_3589 0.37 +0.6 4,002,256 + Pf1N1B4_3589 0.40 -0.5 4,002,257 - Pf1N1B4_3589 0.40 +0.1 4,002,402 + Pf1N1B4_3589 0.54 -1.7 4,002,704 - Pf1N1B4_3589 0.83 +0.9 4,002,721 - Pf1N1B4_3589 0.84 -1.6 4,002,721 - Pf1N1B4_3589 0.84 +0.5 4,002,721 - Pf1N1B4_3589 0.84 +1.0 4,002,721 - Pf1N1B4_3589 0.84 -0.1 4,002,721 - Pf1N1B4_3589 0.84 +1.5 4,002,721 - Pf1N1B4_3589 0.84 -0.7 4,002,721 - Pf1N1B4_3589 0.84 -0.9 4,002,721 - Pf1N1B4_3589 0.84 +0.4 4,002,721 - Pf1N1B4_3589 0.84 -0.3 4,002,721 - Pf1N1B4_3589 0.84 -0.0 4,002,721 - Pf1N1B4_3589 0.84 -0.8 4,002,721 - Pf1N1B4_3589 0.84 +0.3 4,002,915 - -1.7 4,002,934 - +0.0 4,002,934 - -1.1 4,002,951 + -0.1 4,002,952 - -1.2 4,002,952 - +0.4 4,002,952 - -2.6 4,002,952 - -0.8 4,002,952 - -1.1 4,002,952 - +0.5 4,002,957 - -1.6 4,002,957 - -3.6 4,002,957 - -1.0 4,002,957 - -0.3 4,002,957 - -2.1 4,002,957 - -0.7 4,002,957 - -2.0 4,002,957 - -1.1 4,002,959 - -1.9 4,003,009 - -1.3 4,003,009 - -1.3 4,003,009 - -0.6 4,003,045 - -2.1 4,003,089 - Pf1N1B4_3590 0.33 -2.2 4,003,089 - Pf1N1B4_3590 0.33 -0.9 4,003,101 - Pf1N1B4_3590 0.42 +0.9 4,003,101 - Pf1N1B4_3590 0.42 -0.1 4,003,128 + Pf1N1B4_3590 0.62 -1.7 4,003,129 - Pf1N1B4_3590 0.62 -2.1 4,003,145 + Pf1N1B4_3590 0.74 -0.8 4,003,145 + Pf1N1B4_3590 0.74 -1.1 4,003,145 + Pf1N1B4_3590 0.74 -1.3 4,003,145 + Pf1N1B4_3590 0.74 -1.1 4,003,145 + Pf1N1B4_3590 0.74 -2.3 4,003,146 - Pf1N1B4_3590 0.75 +0.6 4,003,146 - Pf1N1B4_3590 0.75 -1.1 4,003,146 - Pf1N1B4_3590 0.75 -1.7 4,003,146 - Pf1N1B4_3590 0.75 +0.5 4,003,172 + +1.5 4,003,172 + -0.1 4,003,172 + -0.8 4,003,172 + -0.8 4,003,173 - -0.9 4,003,173 - -3.9 4,003,173 - -2.1 4,003,173 - -0.3 4,003,173 - +0.3 4,003,173 - -0.9 4,003,173 - +0.1 4,003,173 - -1.0 4,003,173 - -1.3 4,003,444 + Pf1N1B4_3591 0.32 -1.1 4,003,445 - Pf1N1B4_3591 0.32 -1.1 4,003,466 + Pf1N1B4_3591 0.34 -1.3 4,003,466 + Pf1N1B4_3591 0.34 -1.2 4,003,467 - Pf1N1B4_3591 0.34 -0.1 4,003,467 - Pf1N1B4_3591 0.34 -0.1 4,003,481 + Pf1N1B4_3591 0.36 -0.4 4,003,481 + Pf1N1B4_3591 0.36 +0.5 4,003,481 + Pf1N1B4_3591 0.36 -0.4 4,003,481 + Pf1N1B4_3591 0.36 -2.1 4,003,481 + Pf1N1B4_3591 0.36 -0.4 4,003,482 - Pf1N1B4_3591 0.36 -0.5 4,003,482 - Pf1N1B4_3591 0.36 -0.9 4,003,482 - Pf1N1B4_3591 0.36 -2.5 4,003,482 - Pf1N1B4_3591 0.36 -1.8 4,003,547 + Pf1N1B4_3591 0.44 -1.1 4,003,918 + Pf1N1B4_3591 0.86 -1.3 4,003,918 + Pf1N1B4_3591 0.86 -0.6 4,004,033 + -1.8 4,004,033 + -1.9 4,004,033 + -1.6 4,004,033 + +1.3 4,004,033 + -0.7 4,004,033 + -2.6 4,004,034 - -1.2 4,004,034 - -2.3 4,004,034 - -0.5 4,004,034 - -1.6 4,004,034 - -1.5 4,004,034 - +0.9 4,004,034 - -0.9 4,004,034 - -0.1 4,004,034 - -1.6 4,004,034 - -0.4 4,004,034 - -0.1 4,004,050 + +1.0 4,004,050 + -0.1 4,004,050 + -1.7 4,004,051 - -0.1 4,004,051 - +0.5 4,004,083 + +0.9 4,004,097 - +0.1 4,004,163 - -1.0 4,004,163 - -0.1
Or see this region's nucleotide sequence