Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides Pf1N1B4_2723 and Pf1N1B4_2724 are separated by 187 nucleotides Pf1N1B4_2724 and Pf1N1B4_2725 are separated by 435 nucleotides
Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064
_2722
Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268
_2723
Pf1N1B4_2724 - Transcriptional regulator, AsnC family, at 3,062,456 to 3,062,935
_2724
Pf1N1B4_2725 - FIG00954854: hypothetical protein, at 3,063,371 to 3,064,366
_2725
Position (kb)
3062
3063 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.809 kb on + strand, within Pf1N1B4_2722 at 3062.060 kb on + strand at 3062.061 kb on - strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.063 kb on - strand at 3062.063 kb on - strand at 3062.131 kb on + strand, within Pf1N1B4_2723 at 3062.132 kb on - strand, within Pf1N1B4_2723 at 3062.294 kb on + strand at 3062.294 kb on + strand at 3062.295 kb on - strand at 3062.309 kb on + strand at 3062.309 kb on + strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.311 kb on + strand at 3062.311 kb on + strand at 3062.312 kb on - strand at 3062.312 kb on - strand at 3062.361 kb on + strand at 3062.361 kb on + strand at 3062.361 kb on + strand at 3062.362 kb on - strand at 3062.362 kb on - strand at 3062.362 kb on - strand at 3062.506 kb on + strand, within Pf1N1B4_2724 at 3062.507 kb on - strand, within Pf1N1B4_2724 at 3062.507 kb on - strand, within Pf1N1B4_2724 at 3062.600 kb on + strand, within Pf1N1B4_2724 at 3062.600 kb on + strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.601 kb on - strand, within Pf1N1B4_2724 at 3062.613 kb on + strand, within Pf1N1B4_2724 at 3062.613 kb on + strand, within Pf1N1B4_2724 at 3062.614 kb on - strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.704 kb on + strand, within Pf1N1B4_2724 at 3062.705 kb on - strand, within Pf1N1B4_2724 at 3062.705 kb on - strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.730 kb on + strand, within Pf1N1B4_2724 at 3062.731 kb on - strand, within Pf1N1B4_2724 at 3062.731 kb on - strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.790 kb on + strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.791 kb on - strand, within Pf1N1B4_2724 at 3062.891 kb on + strand at 3062.891 kb on + strand at 3062.891 kb on + strand at 3062.892 kb on - strand at 3062.892 kb on - strand at 3062.957 kb on + strand at 3062.971 kb on - strand at 3062.974 kb on + strand at 3063.048 kb on + strand at 3063.054 kb on + strand at 3063.054 kb on + strand at 3063.154 kb on + strand at 3063.155 kb on - strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.195 kb on + strand at 3063.196 kb on - strand at 3063.196 kb on - strand at 3063.265 kb on - strand at 3063.280 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.284 kb on + strand at 3063.285 kb on - strand at 3063.285 kb on - strand at 3063.337 kb on + strand at 3063.337 kb on + strand at 3063.337 kb on + strand at 3063.338 kb on - strand at 3063.338 kb on - strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.411 kb on + strand at 3063.412 kb on - strand at 3063.412 kb on - strand at 3063.412 kb on - strand at 3063.737 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.761 kb on + strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.762 kb on - strand, within Pf1N1B4_2725 at 3063.869 kb on + strand, within Pf1N1B4_2725 at 3063.882 kb on - strand, within Pf1N1B4_2725 at 3063.911 kb on + strand, within Pf1N1B4_2725
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,061,732 + Pf1N1B4_2722 0.48 -0.4 3,061,732 + Pf1N1B4_2722 0.48 -0.8 3,061,732 + Pf1N1B4_2722 0.48 +0.3 3,061,732 + Pf1N1B4_2722 0.48 -1.1 3,061,733 - Pf1N1B4_2722 0.48 -1.2 3,061,733 - Pf1N1B4_2722 0.48 +0.3 3,061,733 - Pf1N1B4_2722 0.48 -0.5 3,061,733 - Pf1N1B4_2722 0.48 -0.2 3,061,733 - Pf1N1B4_2722 0.48 -0.1 3,061,733 - Pf1N1B4_2722 0.48 +1.2 3,061,809 + Pf1N1B4_2722 0.60 +0.1 3,062,060 + +0.2 3,062,061 - -0.8 3,062,062 + +0.7 3,062,062 + -0.5 3,062,062 + +0.2 3,062,062 + +2.5 3,062,062 + -1.4 3,062,062 + -0.8 3,062,063 - +2.8 3,062,063 - +0.2 3,062,131 + Pf1N1B4_2723 0.34 +0.8 3,062,132 - Pf1N1B4_2723 0.35 -0.2 3,062,294 + +0.8 3,062,294 + -0.2 3,062,295 - -0.8 3,062,309 + -1.2 3,062,309 + +0.2 3,062,310 - -0.2 3,062,310 - -0.3 3,062,310 - +0.2 3,062,311 + +0.2 3,062,311 + +0.2 3,062,312 - -0.8 3,062,312 - -0.8 3,062,361 + +2.2 3,062,361 + -0.1 3,062,361 + -0.2 3,062,362 - +0.0 3,062,362 - +0.8 3,062,362 - -0.4 3,062,506 + Pf1N1B4_2724 0.10 +0.8 3,062,507 - Pf1N1B4_2724 0.11 -0.5 3,062,507 - Pf1N1B4_2724 0.11 -2.1 3,062,600 + Pf1N1B4_2724 0.30 -0.8 3,062,600 + Pf1N1B4_2724 0.30 -0.6 3,062,601 - Pf1N1B4_2724 0.30 +0.2 3,062,601 - Pf1N1B4_2724 0.30 +1.2 3,062,601 - Pf1N1B4_2724 0.30 -1.8 3,062,613 + Pf1N1B4_2724 0.33 -1.5 3,062,613 + Pf1N1B4_2724 0.33 -1.1 3,062,614 - Pf1N1B4_2724 0.33 +0.2 3,062,704 + Pf1N1B4_2724 0.52 +0.5 3,062,704 + Pf1N1B4_2724 0.52 -0.8 3,062,704 + Pf1N1B4_2724 0.52 -0.6 3,062,705 - Pf1N1B4_2724 0.52 +0.2 3,062,705 - Pf1N1B4_2724 0.52 -1.0 3,062,730 + Pf1N1B4_2724 0.57 -1.1 3,062,730 + Pf1N1B4_2724 0.57 -0.8 3,062,730 + Pf1N1B4_2724 0.57 -0.5 3,062,731 - Pf1N1B4_2724 0.57 -2.4 3,062,731 - Pf1N1B4_2724 0.57 -0.2 3,062,790 + Pf1N1B4_2724 0.70 -0.4 3,062,790 + Pf1N1B4_2724 0.70 +0.2 3,062,790 + Pf1N1B4_2724 0.70 -1.0 3,062,790 + Pf1N1B4_2724 0.70 -0.2 3,062,790 + Pf1N1B4_2724 0.70 +0.2 3,062,790 + Pf1N1B4_2724 0.70 -0.8 3,062,791 - Pf1N1B4_2724 0.70 -1.5 3,062,791 - Pf1N1B4_2724 0.70 +0.2 3,062,791 - Pf1N1B4_2724 0.70 +0.2 3,062,791 - Pf1N1B4_2724 0.70 -1.1 3,062,791 - Pf1N1B4_2724 0.70 -0.5 3,062,891 + -0.4 3,062,891 + +0.6 3,062,891 + +0.4 3,062,892 - +0.2 3,062,892 - +0.2 3,062,957 + +0.2 3,062,971 - +0.8 3,062,974 + -0.4 3,063,048 + +1.4 3,063,054 + +0.9 3,063,054 + +1.2 3,063,154 + +0.6 3,063,155 - +0.2 3,063,195 + +1.5 3,063,195 + -0.2 3,063,195 + -1.2 3,063,195 + +0.0 3,063,196 - +0.5 3,063,196 - +0.2 3,063,265 - +0.7 3,063,280 + -1.8 3,063,284 + -0.2 3,063,284 + +1.8 3,063,284 + -1.8 3,063,284 + -2.2 3,063,284 + +0.8 3,063,284 + -0.5 3,063,284 + +0.7 3,063,285 - +2.2 3,063,285 - -0.6 3,063,337 + -2.2 3,063,337 + -1.8 3,063,337 + +0.2 3,063,338 - -0.6 3,063,338 - +0.2 3,063,411 + +0.2 3,063,411 + +1.2 3,063,411 + -1.2 3,063,411 + +0.4 3,063,411 + -1.8 3,063,411 + +0.2 3,063,411 + -0.6 3,063,412 - -0.4 3,063,412 - -1.8 3,063,412 - +2.6 3,063,737 + Pf1N1B4_2725 0.37 +0.9 3,063,761 + Pf1N1B4_2725 0.39 +1.4 3,063,761 + Pf1N1B4_2725 0.39 -1.4 3,063,761 + Pf1N1B4_2725 0.39 +0.8 3,063,761 + Pf1N1B4_2725 0.39 +0.2 3,063,761 + Pf1N1B4_2725 0.39 -0.8 3,063,761 + Pf1N1B4_2725 0.39 +2.2 3,063,761 + Pf1N1B4_2725 0.39 +0.2 3,063,761 + Pf1N1B4_2725 0.39 -0.2 3,063,761 + Pf1N1B4_2725 0.39 +1.5 3,063,762 - Pf1N1B4_2725 0.39 +0.4 3,063,762 - Pf1N1B4_2725 0.39 -1.4 3,063,762 - Pf1N1B4_2725 0.39 +2.0 3,063,762 - Pf1N1B4_2725 0.39 +1.5 3,063,762 - Pf1N1B4_2725 0.39 -0.6 3,063,869 + Pf1N1B4_2725 0.50 -0.4 3,063,882 - Pf1N1B4_2725 0.51 -0.6 3,063,911 + Pf1N1B4_2725 0.54 +0.3
Or see this region's nucleotide sequence