Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2113 and Pf1N1B4_2114 are separated by 57 nucleotides Pf1N1B4_2114 and Pf1N1B4_2115 are separated by 167 nucleotides Pf1N1B4_2115 and Pf1N1B4_2116 are separated by 12 nucleotides
Pf1N1B4_2113 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 2,424,927 to 2,427,032
_2113
Pf1N1B4_2114 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 2,427,090 to 2,427,353
_2114
Pf1N1B4_2115 - Guanylate kinase (EC 2.7.4.8), at 2,427,521 to 2,428,141
_2115
Pf1N1B4_2116 - Protein YicC, at 2,428,154 to 2,429,017
_2116
Position (kb)
2427
2428 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2427.022 kb on - strand at 2427.022 kb on - strand at 2427.022 kb on - strand at 2427.022 kb on - strand at 2427.035 kb on + strand at 2427.035 kb on + strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.036 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.071 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.092 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.094 kb on - strand at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.173 kb on - strand, within Pf1N1B4_2114 at 2427.208 kb on - strand, within Pf1N1B4_2114 at 2427.208 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.216 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.325 kb on - strand, within Pf1N1B4_2114 at 2427.357 kb on - strand at 2427.357 kb on - strand at 2427.357 kb on - strand at 2427.357 kb on - strand at 2427.378 kb on - strand at 2427.378 kb on - strand at 2427.378 kb on - strand at 2427.378 kb on - strand at 2427.378 kb on - strand at 2427.474 kb on + strand at 2427.474 kb on + strand at 2427.474 kb on + strand at 2427.474 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.476 kb on + strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.477 kb on - strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.488 kb on + strand at 2427.489 kb on - strand at 2427.489 kb on - strand at 2427.489 kb on - strand at 2427.489 kb on - strand at 2427.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,427,022 - -0.6 2,427,022 - -2.2 2,427,022 - -1.0 2,427,022 - +0.4 2,427,035 + +0.4 2,427,035 + -0.2 2,427,036 - -2.2 2,427,036 - -0.2 2,427,036 - -0.8 2,427,036 - -1.4 2,427,036 - -0.9 2,427,036 - +0.1 2,427,036 - -0.0 2,427,071 - -0.9 2,427,071 - +1.2 2,427,071 - +0.5 2,427,071 - -1.2 2,427,071 - +0.8 2,427,071 - +1.2 2,427,071 - -1.7 2,427,071 - +1.1 2,427,071 - -1.2 2,427,071 - -2.4 2,427,071 - -0.9 2,427,092 - +1.7 2,427,092 - -0.3 2,427,092 - -1.9 2,427,092 - -0.7 2,427,092 - -1.3 2,427,092 - +0.4 2,427,092 - -0.5 2,427,092 - -1.2 2,427,092 - +0.8 2,427,092 - +0.1 2,427,092 - -0.5 2,427,092 - -0.8 2,427,094 - -0.6 2,427,094 - -0.3 2,427,094 - -0.5 2,427,094 - -0.6 2,427,094 - -1.3 2,427,094 - -1.5 2,427,094 - -0.6 2,427,094 - -0.4 2,427,094 - +0.4 2,427,094 - +0.2 2,427,094 - -1.5 2,427,094 - -0.5 2,427,094 - -1.2 2,427,094 - +0.4 2,427,094 - -1.8 2,427,094 - -0.6 2,427,094 - -0.6 2,427,094 - +1.8 2,427,094 - +0.8 2,427,094 - +0.2 2,427,094 - +1.2 2,427,173 - Pf1N1B4_2114 0.31 -2.3 2,427,173 - Pf1N1B4_2114 0.31 -1.2 2,427,173 - Pf1N1B4_2114 0.31 -1.9 2,427,173 - Pf1N1B4_2114 0.31 -0.6 2,427,173 - Pf1N1B4_2114 0.31 -0.3 2,427,173 - Pf1N1B4_2114 0.31 -0.6 2,427,173 - Pf1N1B4_2114 0.31 -2.1 2,427,208 - Pf1N1B4_2114 0.45 +0.1 2,427,208 - Pf1N1B4_2114 0.45 -0.2 2,427,216 - Pf1N1B4_2114 0.48 -0.0 2,427,216 - Pf1N1B4_2114 0.48 -0.8 2,427,216 - Pf1N1B4_2114 0.48 -0.6 2,427,216 - Pf1N1B4_2114 0.48 +2.2 2,427,216 - Pf1N1B4_2114 0.48 -1.4 2,427,216 - Pf1N1B4_2114 0.48 -2.2 2,427,216 - Pf1N1B4_2114 0.48 +1.4 2,427,216 - Pf1N1B4_2114 0.48 -1.6 2,427,216 - Pf1N1B4_2114 0.48 +1.0 2,427,216 - Pf1N1B4_2114 0.48 -1.2 2,427,216 - Pf1N1B4_2114 0.48 +0.4 2,427,216 - Pf1N1B4_2114 0.48 -0.6 2,427,216 - Pf1N1B4_2114 0.48 -1.9 2,427,325 - Pf1N1B4_2114 0.89 +1.0 2,427,325 - Pf1N1B4_2114 0.89 -0.5 2,427,325 - Pf1N1B4_2114 0.89 +0.4 2,427,325 - Pf1N1B4_2114 0.89 +1.0 2,427,325 - Pf1N1B4_2114 0.89 +1.0 2,427,325 - Pf1N1B4_2114 0.89 -2.9 2,427,325 - Pf1N1B4_2114 0.89 -0.8 2,427,357 - +1.0 2,427,357 - -1.6 2,427,357 - +1.0 2,427,357 - -1.2 2,427,378 - -0.6 2,427,378 - -0.8 2,427,378 - -0.3 2,427,378 - -0.5 2,427,378 - -0.6 2,427,474 + +1.2 2,427,474 + -0.6 2,427,474 + -0.0 2,427,474 + +1.1 2,427,476 + -1.2 2,427,476 + -0.3 2,427,476 + +0.9 2,427,476 + -0.5 2,427,476 + -1.2 2,427,476 + -1.2 2,427,476 + -2.6 2,427,476 + +1.7 2,427,476 + +1.4 2,427,476 + +0.1 2,427,476 + -0.6 2,427,476 + +0.1 2,427,476 + -2.3 2,427,476 + -0.2 2,427,476 + -1.0 2,427,476 + +0.4 2,427,476 + +2.7 2,427,476 + -0.4 2,427,476 + +0.6 2,427,476 + +0.6 2,427,476 + -0.2 2,427,476 + -1.0 2,427,476 + -1.2 2,427,476 + -0.2 2,427,477 - -0.6 2,427,477 - -1.4 2,427,477 - +2.0 2,427,477 - -1.6 2,427,477 - +0.8 2,427,477 - +0.4 2,427,477 - -0.3 2,427,477 - -0.2 2,427,477 - +0.3 2,427,488 + +0.4 2,427,488 + +0.7 2,427,488 + +1.1 2,427,488 + +0.4 2,427,488 + -2.4 2,427,488 + +1.7 2,427,488 + -1.1 2,427,488 + +0.4 2,427,488 + -0.1 2,427,488 + +1.1 2,427,488 + +1.6 2,427,488 + +0.4 2,427,488 + -0.4 2,427,488 + -0.6 2,427,488 + +1.4 2,427,488 + +0.4 2,427,488 + -0.2 2,427,488 + +0.1 2,427,489 - +2.4 2,427,489 - -0.8 2,427,489 - -1.4 2,427,489 - -1.2 2,427,522 + +0.7
Or see this region's nucleotide sequence