Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_174 and Pf1N1B4_175 overlap by 1 nucleotides Pf1N1B4_175 and Pf1N1B4_176 are separated by 247 nucleotides
Pf1N1B4_174 - Lipid A export ATP-binding/permease protein MsbA, at 215,454 to 217,172
_174
Pf1N1B4_175 - sigma-54 dependent transcriptional regulator, at 217,172 to 219,076
_175
Pf1N1B4_176 - Acetylpolyamine aminohydrolase, at 219,324 to 220,349
_176
Position (kb) 217
218
219
220 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 216.236 kb on - strand, within Pf1N1B4_174 at 216.281 kb on - strand, within Pf1N1B4_174 at 216.439 kb on + strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.440 kb on - strand, within Pf1N1B4_174 at 216.802 kb on + strand, within Pf1N1B4_174 at 217.100 kb on - strand at 217.190 kb on - strand at 218.173 kb on - strand, within Pf1N1B4_175 at 218.388 kb on + strand, within Pf1N1B4_175 at 218.563 kb on - strand at 218.608 kb on - strand, within Pf1N1B4_175 at 218.608 kb on - strand, within Pf1N1B4_175 at 218.981 kb on + strand at 218.981 kb on + strand at 219.044 kb on + strand at 219.044 kb on + strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.045 kb on - strand at 219.069 kb on - strand at 219.169 kb on - strand at 219.169 kb on - strand at 219.306 kb on + strand at 219.307 kb on - strand at 219.507 kb on - strand, within Pf1N1B4_176 at 219.718 kb on + strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.719 kb on - strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.847 kb on + strand, within Pf1N1B4_176 at 219.848 kb on - strand, within Pf1N1B4_176 at 219.908 kb on - strand, within Pf1N1B4_176 at 220.021 kb on + strand, within Pf1N1B4_176 at 220.022 kb on - strand, within Pf1N1B4_176
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 216,236 - Pf1N1B4_174 0.45 +0.2 216,281 - Pf1N1B4_174 0.48 +0.5 216,439 + Pf1N1B4_174 0.57 +1.2 216,440 - Pf1N1B4_174 0.57 -0.8 216,440 - Pf1N1B4_174 0.57 -0.6 216,440 - Pf1N1B4_174 0.57 -0.2 216,440 - Pf1N1B4_174 0.57 +0.8 216,440 - Pf1N1B4_174 0.57 -0.5 216,802 + Pf1N1B4_174 0.78 +1.2 217,100 - +1.8 217,190 - -2.5 218,173 - Pf1N1B4_175 0.53 +1.0 218,388 + Pf1N1B4_175 0.64 +0.5 218,563 - +0.8 218,608 - Pf1N1B4_175 0.75 +2.4 218,608 - Pf1N1B4_175 0.75 +0.4 218,981 + +0.6 218,981 + +0.3 219,044 + +1.4 219,044 + -1.8 219,045 - +2.4 219,045 - +1.1 219,045 - +0.4 219,069 - +0.4 219,169 - +1.1 219,169 - -0.7 219,306 + -0.2 219,307 - -0.2 219,507 - Pf1N1B4_176 0.18 -0.2 219,718 + Pf1N1B4_176 0.38 -1.6 219,719 - Pf1N1B4_176 0.38 +2.8 219,719 - Pf1N1B4_176 0.38 +0.8 219,719 - Pf1N1B4_176 0.38 -1.5 219,847 + Pf1N1B4_176 0.51 -2.2 219,847 + Pf1N1B4_176 0.51 +1.0 219,848 - Pf1N1B4_176 0.51 +0.2 219,908 - Pf1N1B4_176 0.57 -0.6 220,021 + Pf1N1B4_176 0.68 +2.8 220,022 - Pf1N1B4_176 0.68 +1.8
Or see this region's nucleotide sequence