Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1582 and Pf1N1B4_1583 are separated by 158 nucleotides Pf1N1B4_1583 and Pf1N1B4_1584 overlap by 4 nucleotides Pf1N1B4_1584 and Pf1N1B4_1585 are separated by 71 nucleotides
Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 1,822,609 to 1,824,132
_1582
Pf1N1B4_1583 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 1,824,291 to 1,825,121
_1583
Pf1N1B4_1584 - Histidine ABC transporter, permease protein (TC 3.A.1), at 1,825,118 to 1,825,969
_1584
Pf1N1B4_1585 - Histidine ABC transporter, histidine-binding protein (TC 3.A.1), at 1,826,041 to 1,827,009
_1585
Position (kb)
1825
1826 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.316 kb on - strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583 at 1825.149 kb on + strand at 1825.149 kb on + strand at 1825.150 kb on - strand at 1825.159 kb on + strand at 1825.479 kb on + strand, within Pf1N1B4_1584 at 1825.480 kb on - strand, within Pf1N1B4_1584 at 1825.545 kb on + strand, within Pf1N1B4_1584 at 1825.896 kb on + strand at 1825.952 kb on - strand at 1825.969 kb on + strand at 1826.042 kb on + strand at 1826.042 kb on + strand at 1826.042 kb on + strand at 1826.043 kb on - strand at 1826.043 kb on - strand at 1826.043 kb on - strand at 1826.043 kb on - strand at 1826.348 kb on + strand, within Pf1N1B4_1585 at 1826.349 kb on - strand, within Pf1N1B4_1585 at 1826.351 kb on + strand, within Pf1N1B4_1585 at 1826.351 kb on + strand, within Pf1N1B4_1585 at 1826.351 kb on + strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.352 kb on - strand, within Pf1N1B4_1585 at 1826.378 kb on + strand, within Pf1N1B4_1585 at 1826.378 kb on + strand, within Pf1N1B4_1585 at 1826.378 kb on + strand, within Pf1N1B4_1585 at 1826.379 kb on - strand, within Pf1N1B4_1585 at 1826.379 kb on - strand, within Pf1N1B4_1585 at 1826.379 kb on - strand, within Pf1N1B4_1585 at 1826.379 kb on - strand, within Pf1N1B4_1585 at 1826.435 kb on + strand, within Pf1N1B4_1585 at 1826.435 kb on + strand, within Pf1N1B4_1585 at 1826.435 kb on + strand, within Pf1N1B4_1585 at 1826.436 kb on - strand, within Pf1N1B4_1585 at 1826.436 kb on - strand, within Pf1N1B4_1585 at 1826.436 kb on - strand, within Pf1N1B4_1585 at 1826.453 kb on + strand, within Pf1N1B4_1585 at 1826.454 kb on - strand, within Pf1N1B4_1585 at 1826.454 kb on - strand, within Pf1N1B4_1585 at 1826.454 kb on - strand, within Pf1N1B4_1585 at 1826.510 kb on + strand, within Pf1N1B4_1585 at 1826.511 kb on - strand, within Pf1N1B4_1585 at 1826.511 kb on - strand, within Pf1N1B4_1585 at 1826.558 kb on + strand, within Pf1N1B4_1585 at 1826.558 kb on + strand, within Pf1N1B4_1585 at 1826.558 kb on + strand, within Pf1N1B4_1585 at 1826.558 kb on + strand, within Pf1N1B4_1585 at 1826.559 kb on - strand, within Pf1N1B4_1585 at 1826.559 kb on - strand, within Pf1N1B4_1585 at 1826.559 kb on - strand, within Pf1N1B4_1585 at 1826.559 kb on - strand, within Pf1N1B4_1585 at 1826.559 kb on - strand, within Pf1N1B4_1585 at 1826.649 kb on - strand, within Pf1N1B4_1585 at 1826.649 kb on - strand, within Pf1N1B4_1585 at 1826.649 kb on - strand, within Pf1N1B4_1585 at 1826.649 kb on - strand, within Pf1N1B4_1585 at 1826.649 kb on - strand, within Pf1N1B4_1585 at 1826.831 kb on + strand, within Pf1N1B4_1585
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,824,315 + +0.7 1,824,315 + +0.9 1,824,315 + -0.1 1,824,316 - -0.1 1,824,331 + +3.5 1,824,331 + -1.3 1,824,331 + -0.7 1,824,331 + -0.5 1,824,331 + +0.3 1,824,332 - +0.3 1,824,332 - -0.7 1,824,332 - +0.2 1,824,332 - +0.3 1,824,332 - +0.1 1,824,332 - +0.3 1,824,629 - Pf1N1B4_1583 0.41 +0.3 1,824,629 - Pf1N1B4_1583 0.41 +1.6 1,824,629 - Pf1N1B4_1583 0.41 +1.1 1,824,629 - Pf1N1B4_1583 0.41 -1.3 1,824,629 - Pf1N1B4_1583 0.41 -1.3 1,824,725 - Pf1N1B4_1583 0.52 -0.2 1,824,725 - Pf1N1B4_1583 0.52 +1.1 1,824,826 + Pf1N1B4_1583 0.64 -1.0 1,824,826 + Pf1N1B4_1583 0.64 +0.8 1,824,827 - Pf1N1B4_1583 0.65 -1.0 1,824,827 - Pf1N1B4_1583 0.65 -0.1 1,825,149 + +0.3 1,825,149 + +0.7 1,825,150 - +0.6 1,825,159 + +1.1 1,825,479 + Pf1N1B4_1584 0.42 +0.3 1,825,480 - Pf1N1B4_1584 0.42 +1.3 1,825,545 + Pf1N1B4_1584 0.50 +1.6 1,825,896 + +0.7 1,825,952 - +0.8 1,825,969 + +2.9 1,826,042 + +1.8 1,826,042 + +0.6 1,826,042 + +0.3 1,826,043 - +0.3 1,826,043 - +0.3 1,826,043 - -0.1 1,826,043 - -0.7 1,826,348 + Pf1N1B4_1585 0.32 +0.6 1,826,349 - Pf1N1B4_1585 0.32 +0.3 1,826,351 + Pf1N1B4_1585 0.32 -1.7 1,826,351 + Pf1N1B4_1585 0.32 +1.0 1,826,351 + Pf1N1B4_1585 0.32 +1.3 1,826,352 - Pf1N1B4_1585 0.32 +0.3 1,826,352 - Pf1N1B4_1585 0.32 +0.8 1,826,352 - Pf1N1B4_1585 0.32 +0.9 1,826,352 - Pf1N1B4_1585 0.32 -0.8 1,826,352 - Pf1N1B4_1585 0.32 +0.2 1,826,352 - Pf1N1B4_1585 0.32 +0.6 1,826,378 + Pf1N1B4_1585 0.35 +2.6 1,826,378 + Pf1N1B4_1585 0.35 +1.0 1,826,378 + Pf1N1B4_1585 0.35 +0.1 1,826,379 - Pf1N1B4_1585 0.35 +1.3 1,826,379 - Pf1N1B4_1585 0.35 -1.3 1,826,379 - Pf1N1B4_1585 0.35 +0.7 1,826,379 - Pf1N1B4_1585 0.35 -2.0 1,826,435 + Pf1N1B4_1585 0.41 +1.3 1,826,435 + Pf1N1B4_1585 0.41 +0.0 1,826,435 + Pf1N1B4_1585 0.41 -0.3 1,826,436 - Pf1N1B4_1585 0.41 -1.7 1,826,436 - Pf1N1B4_1585 0.41 -0.2 1,826,436 - Pf1N1B4_1585 0.41 -1.0 1,826,453 + Pf1N1B4_1585 0.43 +0.6 1,826,454 - Pf1N1B4_1585 0.43 -0.9 1,826,454 - Pf1N1B4_1585 0.43 -0.8 1,826,454 - Pf1N1B4_1585 0.43 -0.7 1,826,510 + Pf1N1B4_1585 0.48 +0.6 1,826,511 - Pf1N1B4_1585 0.49 +0.3 1,826,511 - Pf1N1B4_1585 0.49 +2.6 1,826,558 + Pf1N1B4_1585 0.53 +1.3 1,826,558 + Pf1N1B4_1585 0.53 -1.5 1,826,558 + Pf1N1B4_1585 0.53 +0.2 1,826,558 + Pf1N1B4_1585 0.53 -0.7 1,826,559 - Pf1N1B4_1585 0.53 -0.9 1,826,559 - Pf1N1B4_1585 0.53 +0.8 1,826,559 - Pf1N1B4_1585 0.53 +1.6 1,826,559 - Pf1N1B4_1585 0.53 -1.4 1,826,559 - Pf1N1B4_1585 0.53 -1.7 1,826,649 - Pf1N1B4_1585 0.63 -1.8 1,826,649 - Pf1N1B4_1585 0.63 -1.3 1,826,649 - Pf1N1B4_1585 0.63 -1.0 1,826,649 - Pf1N1B4_1585 0.63 +0.3 1,826,649 - Pf1N1B4_1585 0.63 +0.3 1,826,831 + Pf1N1B4_1585 0.82 -0.2
Or see this region's nucleotide sequence