Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1517 and Pf1N1B4_1518 overlap by 4 nucleotides Pf1N1B4_1518 and Pf1N1B4_1519 overlap by 1 nucleotides Pf1N1B4_1519 and Pf1N1B4_1520 overlap by 1 nucleotides
Pf1N1B4_1517 - 3-oxoacyl-[ACP] synthase (EC 2.3.1.41) FabV like, at 1,749,982 to 1,751,178
_1517
Pf1N1B4_1518 - 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.60), at 1,751,175 to 1,751,633
_1518
Pf1N1B4_1519 - 3-oxoacyl-[ACP] reductase (EC 1.1.1.100), at 1,751,633 to 1,752,361
_1519
Pf1N1B4_1520 - FIG138576: 3-oxoacyl-[ACP] synthase (EC 2.3.1.41), at 1,752,361 to 1,753,587
_1520
Position (kb)
1751
1752
1753 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1750.732 kb on - strand, within Pf1N1B4_1517 at 1750.732 kb on - strand, within Pf1N1B4_1517 at 1750.889 kb on + strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.890 kb on - strand, within Pf1N1B4_1517 at 1750.939 kb on - strand, within Pf1N1B4_1517 at 1751.491 kb on + strand, within Pf1N1B4_1518 at 1751.492 kb on - strand, within Pf1N1B4_1518 at 1751.492 kb on - strand, within Pf1N1B4_1518 at 1751.605 kb on - strand at 1751.840 kb on - strand, within Pf1N1B4_1519 at 1751.840 kb on - strand, within Pf1N1B4_1519 at 1751.873 kb on + strand, within Pf1N1B4_1519 at 1751.874 kb on - strand, within Pf1N1B4_1519 at 1751.876 kb on + strand, within Pf1N1B4_1519 at 1751.876 kb on + strand, within Pf1N1B4_1519 at 1751.877 kb on - strand, within Pf1N1B4_1519 at 1751.978 kb on + strand, within Pf1N1B4_1519 at 1751.979 kb on - strand, within Pf1N1B4_1519 at 1751.979 kb on - strand, within Pf1N1B4_1519 at 1752.089 kb on + strand, within Pf1N1B4_1519 at 1752.090 kb on - strand, within Pf1N1B4_1519 at 1752.317 kb on + strand at 1752.317 kb on + strand at 1752.318 kb on - strand at 1752.721 kb on - strand, within Pf1N1B4_1520 at 1752.721 kb on - strand at 1752.721 kb on - strand, within Pf1N1B4_1520 at 1752.721 kb on - strand, within Pf1N1B4_1520 at 1752.763 kb on + strand, within Pf1N1B4_1520 at 1752.764 kb on - strand, within Pf1N1B4_1520 at 1752.764 kb on - strand, within Pf1N1B4_1520 at 1752.877 kb on + strand, within Pf1N1B4_1520 at 1752.883 kb on + strand, within Pf1N1B4_1520 at 1752.884 kb on - strand, within Pf1N1B4_1520 at 1752.976 kb on + strand, within Pf1N1B4_1520 at 1752.976 kb on + strand, within Pf1N1B4_1520 at 1752.977 kb on - strand, within Pf1N1B4_1520 at 1753.249 kb on + strand, within Pf1N1B4_1520 at 1753.250 kb on - strand, within Pf1N1B4_1520
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,750,732 - Pf1N1B4_1517 0.63 +2.3 1,750,732 - Pf1N1B4_1517 0.63 -0.1 1,750,889 + Pf1N1B4_1517 0.76 +0.3 1,750,890 - Pf1N1B4_1517 0.76 +1.3 1,750,890 - Pf1N1B4_1517 0.76 +0.3 1,750,890 - Pf1N1B4_1517 0.76 -0.4 1,750,939 - Pf1N1B4_1517 0.80 -1.0 1,751,491 + Pf1N1B4_1518 0.69 +2.6 1,751,492 - Pf1N1B4_1518 0.69 +1.3 1,751,492 - Pf1N1B4_1518 0.69 +0.3 1,751,605 - +1.9 1,751,840 - Pf1N1B4_1519 0.28 +0.8 1,751,840 - Pf1N1B4_1519 0.28 -0.7 1,751,873 + Pf1N1B4_1519 0.33 -0.1 1,751,874 - Pf1N1B4_1519 0.33 -0.5 1,751,876 + Pf1N1B4_1519 0.33 -2.0 1,751,876 + Pf1N1B4_1519 0.33 +0.3 1,751,877 - Pf1N1B4_1519 0.33 -2.0 1,751,978 + Pf1N1B4_1519 0.47 -1.0 1,751,979 - Pf1N1B4_1519 0.47 +0.3 1,751,979 - Pf1N1B4_1519 0.47 -1.5 1,752,089 + Pf1N1B4_1519 0.63 -1.5 1,752,090 - Pf1N1B4_1519 0.63 +0.9 1,752,317 + +1.0 1,752,317 + +1.9 1,752,318 - -1.0 1,752,721 - Pf1N1B4_1520 0.29 +3.1 1,752,721 - +0.3 1,752,721 - Pf1N1B4_1520 0.29 +1.6 1,752,721 - Pf1N1B4_1520 0.29 -0.5 1,752,763 + Pf1N1B4_1520 0.33 +1.8 1,752,764 - Pf1N1B4_1520 0.33 +2.6 1,752,764 - Pf1N1B4_1520 0.33 -1.7 1,752,877 + Pf1N1B4_1520 0.42 +3.9 1,752,883 + Pf1N1B4_1520 0.43 -0.9 1,752,884 - Pf1N1B4_1520 0.43 +0.3 1,752,976 + Pf1N1B4_1520 0.50 +0.3 1,752,976 + Pf1N1B4_1520 0.50 +0.8 1,752,977 - Pf1N1B4_1520 0.50 +0.3 1,753,249 + Pf1N1B4_1520 0.72 -2.0 1,753,250 - Pf1N1B4_1520 0.72 -0.2
Or see this region's nucleotide sequence