Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1292 and Pf1N1B4_1293 are separated by 19 nucleotides Pf1N1B4_1293 and Pf1N1B4_1294 overlap by 4 nucleotides
Pf1N1B4_1292 - DNA-damage-inducible protein F, at 1,472,913 to 1,474,253
_1292
Pf1N1B4_1293 - hypothetical protein, at 1,474,273 to 1,475,070
_1293
Pf1N1B4_1294 - VgrG protein, at 1,475,067 to 1,476,122
_1294
Position (kb)
1474
1475
1476 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1473.803 kb on - strand at 1473.803 kb on - strand, within Pf1N1B4_1292 at 1473.803 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.986 kb on - strand, within Pf1N1B4_1292 at 1473.987 kb on + strand, within Pf1N1B4_1292 at 1473.987 kb on + strand, within Pf1N1B4_1292 at 1473.988 kb on - strand, within Pf1N1B4_1292 at 1473.996 kb on + strand, within Pf1N1B4_1292 at 1473.997 kb on - strand, within Pf1N1B4_1292 at 1474.032 kb on + strand, within Pf1N1B4_1292 at 1474.032 kb on + strand, within Pf1N1B4_1292 at 1474.032 kb on + strand, within Pf1N1B4_1292 at 1474.033 kb on - strand, within Pf1N1B4_1292 at 1474.088 kb on + strand at 1474.089 kb on - strand, within Pf1N1B4_1292 at 1474.218 kb on + strand at 1474.218 kb on + strand at 1474.219 kb on - strand at 1474.219 kb on - strand at 1474.442 kb on + strand, within Pf1N1B4_1293 at 1474.442 kb on + strand, within Pf1N1B4_1293 at 1474.443 kb on - strand, within Pf1N1B4_1293 at 1474.443 kb on - strand, within Pf1N1B4_1293 at 1474.460 kb on + strand at 1474.461 kb on - strand, within Pf1N1B4_1293 at 1474.712 kb on + strand, within Pf1N1B4_1293 at 1474.712 kb on + strand at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.733 kb on + strand, within Pf1N1B4_1293 at 1474.734 kb on - strand, within Pf1N1B4_1293 at 1474.736 kb on + strand, within Pf1N1B4_1293 at 1475.601 kb on - strand, within Pf1N1B4_1294 at 1475.602 kb on + strand, within Pf1N1B4_1294 at 1475.603 kb on - strand, within Pf1N1B4_1294 at 1475.890 kb on + strand, within Pf1N1B4_1294 at 1476.030 kb on + strand at 1476.031 kb on - strand at 1476.031 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,473,803 - +1.2 1,473,803 - Pf1N1B4_1292 0.66 -0.2 1,473,803 - Pf1N1B4_1292 0.66 +1.4 1,473,986 - Pf1N1B4_1292 0.80 +0.8 1,473,986 - Pf1N1B4_1292 0.80 -0.4 1,473,986 - Pf1N1B4_1292 0.80 -0.8 1,473,986 - Pf1N1B4_1292 0.80 +0.2 1,473,986 - Pf1N1B4_1292 0.80 +0.8 1,473,986 - Pf1N1B4_1292 0.80 -2.1 1,473,986 - Pf1N1B4_1292 0.80 +2.0 1,473,987 + Pf1N1B4_1292 0.80 -0.4 1,473,987 + Pf1N1B4_1292 0.80 -0.8 1,473,988 - Pf1N1B4_1292 0.80 -0.1 1,473,996 + Pf1N1B4_1292 0.81 -0.5 1,473,997 - Pf1N1B4_1292 0.81 +1.6 1,474,032 + Pf1N1B4_1292 0.83 +0.4 1,474,032 + Pf1N1B4_1292 0.83 +0.2 1,474,032 + Pf1N1B4_1292 0.83 -0.8 1,474,033 - Pf1N1B4_1292 0.84 -1.1 1,474,088 + -1.4 1,474,089 - Pf1N1B4_1292 0.88 -0.3 1,474,218 + +0.2 1,474,218 + -0.4 1,474,219 - +0.2 1,474,219 - +1.0 1,474,442 + Pf1N1B4_1293 0.21 -1.4 1,474,442 + Pf1N1B4_1293 0.21 +0.0 1,474,443 - Pf1N1B4_1293 0.21 -0.9 1,474,443 - Pf1N1B4_1293 0.21 -2.8 1,474,460 + -2.1 1,474,461 - Pf1N1B4_1293 0.24 -0.2 1,474,712 + Pf1N1B4_1293 0.55 -2.3 1,474,712 + +0.2 1,474,733 + Pf1N1B4_1293 0.58 -0.7 1,474,733 + Pf1N1B4_1293 0.58 +0.2 1,474,733 + Pf1N1B4_1293 0.58 +0.2 1,474,734 - Pf1N1B4_1293 0.58 -0.8 1,474,736 + Pf1N1B4_1293 0.58 +2.5 1,475,601 - Pf1N1B4_1294 0.51 -0.6 1,475,602 + Pf1N1B4_1294 0.51 +0.6 1,475,603 - Pf1N1B4_1294 0.51 -0.6 1,475,890 + Pf1N1B4_1294 0.78 +0.2 1,476,030 + +2.5 1,476,031 - +0.6 1,476,031 - +0.2
Or see this region's nucleotide sequence