Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1179 and Pf1N1B4_1180 are separated by 97 nucleotides Pf1N1B4_1180 and Pf1N1B4_1181 are separated by 61 nucleotides Pf1N1B4_1181 and Pf1N1B4_1182 overlap by 8 nucleotides Pf1N1B4_1182 and Pf1N1B4_1183 are separated by 7 nucleotides
Pf1N1B4_1179 - Ferric uptake regulation protein FUR, at 1,331,497 to 1,331,901
_1179
Pf1N1B4_1180 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 1,331,999 to 1,332,526
_1180
Pf1N1B4_1181 - UPF0125 protein yfjF, at 1,332,588 to 1,332,902
_1181
Pf1N1B4_1182 - Putative oligoketide cyclase/dehydratase or lipid transport protein YfjG, at 1,332,895 to 1,333,329
_1182
Pf1N1B4_1183 - Sodium-dependent transporter family protein, at 1,333,337 to 1,334,629
_1183
Position (kb)
1332
1333 Strain fitness (log2 ratio)
-2
-1
0
1 at 1332.373 kb on + strand, within Pf1N1B4_1180 at 1332.373 kb on + strand, within Pf1N1B4_1180 at 1332.524 kb on + strand at 1332.525 kb on - strand at 1332.525 kb on - strand at 1332.540 kb on - strand at 1332.792 kb on + strand, within Pf1N1B4_1181 at 1332.847 kb on - strand, within Pf1N1B4_1181 at 1332.868 kb on + strand at 1332.868 kb on + strand, within Pf1N1B4_1181 at 1332.868 kb on + strand, within Pf1N1B4_1181 at 1332.869 kb on - strand, within Pf1N1B4_1181 at 1332.869 kb on - strand, within Pf1N1B4_1181 at 1332.869 kb on - strand, within Pf1N1B4_1181 at 1332.869 kb on - strand, within Pf1N1B4_1181 at 1332.869 kb on - strand, within Pf1N1B4_1181 at 1333.339 kb on - strand at 1333.339 kb on - strand at 1333.339 kb on - strand at 1333.339 kb on - strand at 1333.339 kb on - strand at 1333.390 kb on - strand at 1333.582 kb on - strand, within Pf1N1B4_1183 at 1333.582 kb on - strand, within Pf1N1B4_1183 at 1333.633 kb on + strand, within Pf1N1B4_1183 at 1333.633 kb on + strand, within Pf1N1B4_1183 at 1333.634 kb on - strand, within Pf1N1B4_1183 at 1333.693 kb on + strand, within Pf1N1B4_1183 at 1333.731 kb on + strand, within Pf1N1B4_1183 at 1333.731 kb on + strand, within Pf1N1B4_1183 at 1333.731 kb on + strand, within Pf1N1B4_1183 at 1333.802 kb on + strand, within Pf1N1B4_1183 at 1333.802 kb on + strand, within Pf1N1B4_1183 at 1333.802 kb on + strand, within Pf1N1B4_1183 at 1333.802 kb on + strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.803 kb on - strand, within Pf1N1B4_1183 at 1333.826 kb on + strand, within Pf1N1B4_1183 at 1333.826 kb on + strand, within Pf1N1B4_1183 at 1333.826 kb on + strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183 at 1333.827 kb on - strand, within Pf1N1B4_1183
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,332,373 + Pf1N1B4_1180 0.71 +0.3 1,332,373 + Pf1N1B4_1180 0.71 +0.1 1,332,524 + -0.9 1,332,525 - -0.4 1,332,525 - +1.0 1,332,540 - +0.6 1,332,792 + Pf1N1B4_1181 0.65 -0.9 1,332,847 - Pf1N1B4_1181 0.82 -0.9 1,332,868 + +1.1 1,332,868 + Pf1N1B4_1181 0.89 +0.9 1,332,868 + Pf1N1B4_1181 0.89 -0.6 1,332,869 - Pf1N1B4_1181 0.89 -0.7 1,332,869 - Pf1N1B4_1181 0.89 -0.9 1,332,869 - Pf1N1B4_1181 0.89 +0.7 1,332,869 - Pf1N1B4_1181 0.89 -0.4 1,332,869 - Pf1N1B4_1181 0.89 -0.1 1,333,339 - +1.1 1,333,339 - -1.4 1,333,339 - +0.6 1,333,339 - +0.6 1,333,339 - -2.7 1,333,390 - -0.6 1,333,582 - Pf1N1B4_1183 0.19 +0.1 1,333,582 - Pf1N1B4_1183 0.19 +0.6 1,333,633 + Pf1N1B4_1183 0.23 -1.2 1,333,633 + Pf1N1B4_1183 0.23 +0.7 1,333,634 - Pf1N1B4_1183 0.23 -0.9 1,333,693 + Pf1N1B4_1183 0.28 +1.7 1,333,731 + Pf1N1B4_1183 0.30 -0.2 1,333,731 + Pf1N1B4_1183 0.30 -0.7 1,333,731 + Pf1N1B4_1183 0.30 -0.4 1,333,802 + Pf1N1B4_1183 0.36 -1.9 1,333,802 + Pf1N1B4_1183 0.36 +0.1 1,333,802 + Pf1N1B4_1183 0.36 -0.9 1,333,802 + Pf1N1B4_1183 0.36 +0.4 1,333,803 - Pf1N1B4_1183 0.36 +0.7 1,333,803 - Pf1N1B4_1183 0.36 -0.3 1,333,803 - Pf1N1B4_1183 0.36 -0.9 1,333,803 - Pf1N1B4_1183 0.36 +0.7 1,333,803 - Pf1N1B4_1183 0.36 -0.1 1,333,803 - Pf1N1B4_1183 0.36 +0.7 1,333,826 + Pf1N1B4_1183 0.38 +0.1 1,333,826 + Pf1N1B4_1183 0.38 +1.7 1,333,826 + Pf1N1B4_1183 0.38 -1.4 1,333,827 - Pf1N1B4_1183 0.38 -0.1 1,333,827 - Pf1N1B4_1183 0.38 +0.7 1,333,827 - Pf1N1B4_1183 0.38 +0.6 1,333,827 - Pf1N1B4_1183 0.38 +0.1 1,333,827 - Pf1N1B4_1183 0.38 +0.7 1,333,827 - Pf1N1B4_1183 0.38 -0.6
Or see this region's nucleotide sequence