Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_115

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_114 and Pf1N1B4_115 are separated by 169 nucleotidesPf1N1B4_115 and Pf1N1B4_116 are separated by 113 nucleotides Pf1N1B4_114 - Diaminopimelate epimerase (EC 5.1.1.7), at 118,869 to 119,858 _114 Pf1N1B4_115 - Membrane protein mosC, at 120,028 to 121,179 _115 Pf1N1B4_116 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 121,293 to 123,725 _116 Position (kb) 120 121 122Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 119.164 kb on + strand, within Pf1N1B4_114at 119.164 kb on + strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.165 kb on - strand, within Pf1N1B4_114at 119.693 kb on + strand, within Pf1N1B4_114at 119.694 kb on - strand, within Pf1N1B4_114at 119.824 kb on + strandat 119.825 kb on - strandat 119.825 kb on - strandat 119.825 kb on - strandat 119.828 kb on + strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.829 kb on - strandat 119.845 kb on + strandat 119.846 kb on - strandat 119.846 kb on - strandat 119.872 kb on - strandat 119.872 kb on - strandat 119.872 kb on - strandat 120.027 kb on + strandat 120.145 kb on + strand, within Pf1N1B4_115at 120.330 kb on + strand, within Pf1N1B4_115at 120.843 kb on + strand, within Pf1N1B4_115at 120.979 kb on + strand, within Pf1N1B4_115at 121.049 kb on - strand, within Pf1N1B4_115at 121.177 kb on + strandat 121.178 kb on - strandat 121.205 kb on + strandat 121.205 kb on + strandat 121.210 kb on + strandat 121.210 kb on + strandat 121.281 kb on + strandat 121.342 kb on + strandat 121.342 kb on + strandat 121.342 kb on + strandat 121.342 kb on + strandat 121.342 kb on + strandat 121.343 kb on - strandat 121.343 kb on - strandat 121.343 kb on - strandat 121.378 kb on + strandat 121.378 kb on + strandat 121.379 kb on - strandat 121.522 kb on + strandat 121.522 kb on + strandat 121.523 kb on - strandat 121.523 kb on - strandat 121.604 kb on - strand, within Pf1N1B4_116at 121.604 kb on - strand, within Pf1N1B4_116at 121.604 kb on - strand, within Pf1N1B4_116at 121.658 kb on - strand, within Pf1N1B4_116at 121.658 kb on - strand, within Pf1N1B4_116at 121.666 kb on - strand, within Pf1N1B4_116at 121.718 kb on - strand, within Pf1N1B4_116at 121.849 kb on + strand, within Pf1N1B4_116at 121.850 kb on - strand, within Pf1N1B4_116at 121.876 kb on + strand, within Pf1N1B4_116at 121.876 kb on + strand, within Pf1N1B4_116at 121.876 kb on + strand, within Pf1N1B4_116at 121.915 kb on + strand, within Pf1N1B4_116at 121.933 kb on + strand, within Pf1N1B4_116at 121.934 kb on - strand, within Pf1N1B4_116at 122.006 kb on - strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.068 kb on + strand, within Pf1N1B4_116at 122.069 kb on - strand, within Pf1N1B4_116at 122.069 kb on - strand, within Pf1N1B4_116at 122.069 kb on - strand, within Pf1N1B4_116at 122.069 kb on - strand, within Pf1N1B4_116at 122.069 kb on - strand, within Pf1N1B4_116

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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119,164 + Pf1N1B4_114 0.30 +0.1
119,164 + Pf1N1B4_114 0.30 +2.3
119,165 - Pf1N1B4_114 0.30 +0.5
119,165 - Pf1N1B4_114 0.30 -0.6
119,165 - Pf1N1B4_114 0.30 +0.6
119,693 + Pf1N1B4_114 0.83 -1.2
119,694 - Pf1N1B4_114 0.83 +0.8
119,824 + +0.2
119,825 - +2.3
119,825 - +0.3
119,825 - +3.8
119,828 + -2.8
119,829 - +0.1
119,829 - +0.3
119,829 - -0.6
119,829 - -1.2
119,845 + -1.6
119,846 - +0.6
119,846 - -2.2
119,872 - -1.5
119,872 - +3.1
119,872 - -1.2
120,027 + -1.0
120,145 + Pf1N1B4_115 0.10 +1.3
120,330 + Pf1N1B4_115 0.26 -1.6
120,843 + Pf1N1B4_115 0.71 +1.3
120,979 + Pf1N1B4_115 0.83 -1.2
121,049 - Pf1N1B4_115 0.89 +1.0
121,177 + +0.2
121,178 - -1.2
121,205 + +0.3
121,205 + -0.2
121,210 + +0.2
121,210 + +0.5
121,281 + -0.2
121,342 + +1.8
121,342 + +1.3
121,342 + +0.4
121,342 + +0.0
121,342 + +1.3
121,343 - -1.8
121,343 - -0.8
121,343 - -0.6
121,378 + +0.1
121,378 + -0.0
121,379 - -1.2
121,522 + -0.3
121,522 + +0.8
121,523 - +0.6
121,523 - -0.7
121,604 - Pf1N1B4_116 0.13 +1.8
121,604 - Pf1N1B4_116 0.13 +0.8
121,604 - Pf1N1B4_116 0.13 -1.8
121,658 - Pf1N1B4_116 0.15 +0.2
121,658 - Pf1N1B4_116 0.15 +0.7
121,666 - Pf1N1B4_116 0.15 +1.3
121,718 - Pf1N1B4_116 0.17 +1.1
121,849 + Pf1N1B4_116 0.23 -0.8
121,850 - Pf1N1B4_116 0.23 +0.1
121,876 + Pf1N1B4_116 0.24 +0.5
121,876 + Pf1N1B4_116 0.24 -0.6
121,876 + Pf1N1B4_116 0.24 -1.6
121,915 + Pf1N1B4_116 0.26 -2.2
121,933 + Pf1N1B4_116 0.26 -2.1
121,934 - Pf1N1B4_116 0.26 -1.8
122,006 - Pf1N1B4_116 0.29 +1.3
122,068 + Pf1N1B4_116 0.32 -2.6
122,068 + Pf1N1B4_116 0.32 +2.1
122,068 + Pf1N1B4_116 0.32 +0.6
122,068 + Pf1N1B4_116 0.32 -0.2
122,068 + Pf1N1B4_116 0.32 -0.2
122,068 + Pf1N1B4_116 0.32 +0.4
122,069 - Pf1N1B4_116 0.32 +1.8
122,069 - Pf1N1B4_116 0.32 +1.8
122,069 - Pf1N1B4_116 0.32 -1.2
122,069 - Pf1N1B4_116 0.32 -2.8
122,069 - Pf1N1B4_116 0.32 +0.3

Or see this region's nucleotide sequence