Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1109 and Pf1N1B4_1110 are separated by 59 nucleotides Pf1N1B4_1110 and Pf1N1B4_1111 are separated by 235 nucleotides
Pf1N1B4_1109 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) (from data) , at 1,242,992 to 1,244,437
_1109
Pf1N1B4_1110 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41) (from data) , at 1,244,497 to 1,245,408
_1110
Pf1N1B4_1111 - Phosphatidylcholine-hydrolyzing phospholipase C (EC 3.1.4.3), at 1,245,644 to 1,246,963
_1111
Position (kb)
1244
1245
1246 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1243.605 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.064 kb on + strand, within Pf1N1B4_1109 at 1244.065 kb on - strand, within Pf1N1B4_1109 at 1244.065 kb on - strand, within Pf1N1B4_1109 at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.409 kb on + strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.410 kb on - strand at 1244.498 kb on + strand at 1244.540 kb on + strand at 1244.591 kb on + strand, within Pf1N1B4_1110 at 1244.591 kb on + strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.592 kb on - strand, within Pf1N1B4_1110 at 1244.777 kb on + strand, within Pf1N1B4_1110 at 1244.778 kb on - strand, within Pf1N1B4_1110 at 1244.814 kb on - strand at 1244.843 kb on + strand, within Pf1N1B4_1110 at 1244.844 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1244.988 kb on - strand, within Pf1N1B4_1110 at 1245.008 kb on - strand, within Pf1N1B4_1110 at 1245.068 kb on + strand, within Pf1N1B4_1110 at 1245.069 kb on - strand, within Pf1N1B4_1110 at 1245.069 kb on - strand, within Pf1N1B4_1110 at 1245.122 kb on + strand, within Pf1N1B4_1110 at 1245.123 kb on - strand, within Pf1N1B4_1110 at 1245.152 kb on - strand, within Pf1N1B4_1110 at 1245.311 kb on + strand, within Pf1N1B4_1110 at 1245.311 kb on + strand, within Pf1N1B4_1110 at 1245.427 kb on - strand at 1245.492 kb on + strand at 1245.546 kb on - strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.574 kb on + strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.575 kb on - strand at 1245.595 kb on + strand at 1245.617 kb on + strand at 1245.617 kb on + strand at 1245.618 kb on - strand at 1245.621 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.628 kb on + strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.629 kb on - strand at 1245.639 kb on + strand at 1245.640 kb on - strand at 1245.640 kb on - strand at 1245.654 kb on + strand at 1245.656 kb on + strand at 1245.677 kb on - strand at 1245.928 kb on - strand, within Pf1N1B4_1111 at 1245.928 kb on - strand, within Pf1N1B4_1111 at 1245.928 kb on - strand at 1245.940 kb on - strand, within Pf1N1B4_1111 at 1245.962 kb on + strand, within Pf1N1B4_1111 at 1245.962 kb on + strand at 1245.963 kb on - strand, within Pf1N1B4_1111 at 1246.058 kb on - strand, within Pf1N1B4_1111 at 1246.190 kb on + strand, within Pf1N1B4_1111 at 1246.191 kb on - strand, within Pf1N1B4_1111 at 1246.191 kb on - strand at 1246.207 kb on + strand, within Pf1N1B4_1111 at 1246.207 kb on + strand, within Pf1N1B4_1111 at 1246.208 kb on - strand, within Pf1N1B4_1111 at 1246.208 kb on - strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.337 kb on + strand, within Pf1N1B4_1111 at 1246.338 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,243,605 + Pf1N1B4_1109 0.42 +0.0 1,244,064 + Pf1N1B4_1109 0.74 -0.3 1,244,064 + Pf1N1B4_1109 0.74 +0.6 1,244,064 + Pf1N1B4_1109 0.74 +0.3 1,244,064 + Pf1N1B4_1109 0.74 +0.9 1,244,064 + Pf1N1B4_1109 0.74 -1.2 1,244,065 - Pf1N1B4_1109 0.74 +2.5 1,244,065 - Pf1N1B4_1109 0.74 +0.7 1,244,409 + -0.4 1,244,409 + -2.2 1,244,409 + -2.2 1,244,409 + +1.1 1,244,409 + +0.3 1,244,409 + +2.5 1,244,409 + -1.2 1,244,409 + +0.1 1,244,409 + +0.8 1,244,409 + -0.1 1,244,409 + -0.1 1,244,409 + +0.5 1,244,410 - +0.1 1,244,410 - -0.0 1,244,410 - -1.2 1,244,410 - +1.1 1,244,410 - +0.6 1,244,410 - -0.6 1,244,410 - +0.9 1,244,498 + -0.7 1,244,540 + +1.1 1,244,591 + Pf1N1B4_1110 0.10 +2.0 1,244,591 + Pf1N1B4_1110 0.10 -0.4 1,244,592 - Pf1N1B4_1110 0.10 +1.2 1,244,592 - Pf1N1B4_1110 0.10 -0.2 1,244,592 - Pf1N1B4_1110 0.10 +2.0 1,244,777 + Pf1N1B4_1110 0.31 +0.1 1,244,778 - Pf1N1B4_1110 0.31 -2.2 1,244,814 - -0.4 1,244,843 + Pf1N1B4_1110 0.38 +0.4 1,244,844 - Pf1N1B4_1110 0.38 +1.4 1,244,988 - Pf1N1B4_1110 0.54 +1.1 1,244,988 - Pf1N1B4_1110 0.54 +0.9 1,244,988 - Pf1N1B4_1110 0.54 +1.7 1,245,008 - Pf1N1B4_1110 0.56 +0.7 1,245,068 + Pf1N1B4_1110 0.63 +3.5 1,245,069 - Pf1N1B4_1110 0.63 +2.3 1,245,069 - Pf1N1B4_1110 0.63 +0.1 1,245,122 + Pf1N1B4_1110 0.69 +1.1 1,245,123 - Pf1N1B4_1110 0.69 +0.3 1,245,152 - Pf1N1B4_1110 0.72 -0.2 1,245,311 + Pf1N1B4_1110 0.89 +0.6 1,245,311 + Pf1N1B4_1110 0.89 -0.9 1,245,427 - +1.5 1,245,492 + +0.4 1,245,546 - +1.7 1,245,574 + +0.7 1,245,574 + +1.7 1,245,574 + +0.1 1,245,574 + +1.1 1,245,574 + -0.7 1,245,574 + +0.7 1,245,575 - +0.4 1,245,575 - -0.9 1,245,575 - +0.1 1,245,575 - -0.9 1,245,575 - -1.6 1,245,575 - -0.9 1,245,575 - +0.1 1,245,595 + +0.0 1,245,617 + -0.0 1,245,617 + -0.2 1,245,618 - +0.1 1,245,621 + -1.4 1,245,628 + -0.1 1,245,628 + -0.9 1,245,628 + +1.7 1,245,628 + -2.2 1,245,628 + +1.7 1,245,628 + -1.8 1,245,628 + -2.4 1,245,629 - -0.9 1,245,629 - +0.7 1,245,629 - -0.3 1,245,629 - -0.4 1,245,629 - +1.6 1,245,639 + +0.4 1,245,640 - -0.4 1,245,640 - +1.1 1,245,654 + +1.1 1,245,656 + +0.9 1,245,677 - -1.4 1,245,928 - Pf1N1B4_1111 0.22 -0.9 1,245,928 - Pf1N1B4_1111 0.22 -1.9 1,245,928 - +0.1 1,245,940 - Pf1N1B4_1111 0.22 -0.2 1,245,962 + Pf1N1B4_1111 0.24 -0.1 1,245,962 + -0.9 1,245,963 - Pf1N1B4_1111 0.24 +1.3 1,246,058 - Pf1N1B4_1111 0.31 +1.3 1,246,190 + Pf1N1B4_1111 0.41 -0.4 1,246,191 - Pf1N1B4_1111 0.41 -0.1 1,246,191 - -0.9 1,246,207 + Pf1N1B4_1111 0.43 +0.1 1,246,207 + Pf1N1B4_1111 0.43 -1.9 1,246,208 - Pf1N1B4_1111 0.43 +1.5 1,246,208 - Pf1N1B4_1111 0.43 -0.4 1,246,337 + Pf1N1B4_1111 0.53 -0.3 1,246,337 + Pf1N1B4_1111 0.53 +2.7 1,246,337 + Pf1N1B4_1111 0.53 +0.9 1,246,337 + Pf1N1B4_1111 0.53 +1.1 1,246,337 + Pf1N1B4_1111 0.53 -0.3 1,246,338 - +2.1
Or see this region's nucleotide sequence