Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1046 and Pf1N1B4_1047 overlap by 18 nucleotides Pf1N1B4_1047 and Pf1N1B4_1048 are separated by 198 nucleotides Pf1N1B4_1048 and Pf1N1B4_1049 are separated by 64 nucleotides
Pf1N1B4_1046 - Thioredoxin reductase (EC 1.8.1.9), at 1,182,833 to 1,183,789
_1046
Pf1N1B4_1047 - hypothetical protein, at 1,183,772 to 1,183,906
_1047
Pf1N1B4_1048 - Sulfate transporter, CysZ-type, at 1,184,105 to 1,184,860
_1048
Pf1N1B4_1049 - Glycosyl transferase, group 1 family protein, at 1,184,925 to 1,186,100
_1049
Position (kb)
1184
1185 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.904 kb on + strand at 1183.971 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1183.999 kb on + strand at 1184.000 kb on - strand at 1184.000 kb on - strand at 1184.000 kb on - strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.001 kb on + strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.002 kb on - strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.003 kb on + strand at 1184.004 kb on - strand at 1184.016 kb on + strand at 1184.016 kb on + strand at 1184.016 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.022 kb on + strand at 1184.135 kb on + strand at 1184.191 kb on - strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.234 kb on + strand, within Pf1N1B4_1048 at 1184.235 kb on - strand, within Pf1N1B4_1048 at 1184.235 kb on - strand at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.257 kb on + strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.258 kb on - strand, within Pf1N1B4_1048 at 1184.312 kb on + strand, within Pf1N1B4_1048 at 1184.312 kb on + strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.313 kb on - strand, within Pf1N1B4_1048 at 1184.942 kb on + strand at 1184.942 kb on + strand at 1184.942 kb on + strand at 1185.078 kb on - strand, within Pf1N1B4_1049 at 1185.207 kb on + strand, within Pf1N1B4_1049 at 1185.208 kb on - strand, within Pf1N1B4_1049 at 1185.208 kb on - strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand, within Pf1N1B4_1049 at 1185.306 kb on + strand at 1185.307 kb on - strand, within Pf1N1B4_1049 at 1185.307 kb on - strand, within Pf1N1B4_1049 at 1185.354 kb on + strand, within Pf1N1B4_1049 at 1185.354 kb on + strand, within Pf1N1B4_1049 at 1185.540 kb on - strand, within Pf1N1B4_1049 at 1185.540 kb on - strand, within Pf1N1B4_1049 at 1185.557 kb on - strand, within Pf1N1B4_1049 at 1185.557 kb on - strand, within Pf1N1B4_1049 at 1185.625 kb on - strand, within Pf1N1B4_1049 at 1185.625 kb on - strand, within Pf1N1B4_1049 at 1185.748 kb on - strand, within Pf1N1B4_1049 at 1185.843 kb on + strand, within Pf1N1B4_1049
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,183,904 + -3.0 1,183,904 + -0.9 1,183,904 + -0.5 1,183,904 + +1.7 1,183,971 + -0.4 1,183,999 + +0.5 1,183,999 + +0.5 1,183,999 + -0.9 1,183,999 + -0.7 1,183,999 + +0.7 1,183,999 + +0.1 1,183,999 + -1.4 1,183,999 + +0.5 1,183,999 + -0.5 1,183,999 + +0.2 1,184,000 - +0.1 1,184,000 - +0.9 1,184,000 - +0.1 1,184,001 + +0.1 1,184,001 + +0.9 1,184,001 + +2.0 1,184,001 + -0.8 1,184,001 + -1.0 1,184,001 + -2.0 1,184,001 + +0.4 1,184,001 + -0.0 1,184,001 + +0.5 1,184,001 + +2.6 1,184,002 - -0.6 1,184,002 - +1.2 1,184,002 - +1.4 1,184,002 - -0.5 1,184,002 - +1.1 1,184,003 + -0.3 1,184,003 + -0.6 1,184,003 + +1.1 1,184,003 + +1.1 1,184,003 + +0.5 1,184,003 + +0.7 1,184,004 - +0.2 1,184,016 + -1.9 1,184,016 + -0.6 1,184,016 + +0.9 1,184,022 + +0.1 1,184,022 + -0.1 1,184,022 + -0.9 1,184,022 + -0.9 1,184,022 + -0.5 1,184,022 + -0.8 1,184,022 + +0.5 1,184,022 + -0.1 1,184,022 + +0.7 1,184,135 + -0.4 1,184,191 - Pf1N1B4_1048 0.11 -0.9 1,184,234 + Pf1N1B4_1048 0.17 +0.6 1,184,234 + Pf1N1B4_1048 0.17 +0.4 1,184,234 + Pf1N1B4_1048 0.17 +0.1 1,184,234 + Pf1N1B4_1048 0.17 +0.1 1,184,235 - Pf1N1B4_1048 0.17 +1.3 1,184,235 - +0.1 1,184,257 + Pf1N1B4_1048 0.20 +0.5 1,184,257 + Pf1N1B4_1048 0.20 +1.9 1,184,257 + Pf1N1B4_1048 0.20 +1.9 1,184,258 - Pf1N1B4_1048 0.20 -0.3 1,184,258 - Pf1N1B4_1048 0.20 +1.2 1,184,258 - Pf1N1B4_1048 0.20 +0.1 1,184,312 + Pf1N1B4_1048 0.27 -0.5 1,184,312 + Pf1N1B4_1048 0.27 +1.9 1,184,313 - Pf1N1B4_1048 0.28 -1.2 1,184,313 - Pf1N1B4_1048 0.28 +0.9 1,184,313 - Pf1N1B4_1048 0.28 -1.5 1,184,942 + +0.4 1,184,942 + -0.9 1,184,942 + -1.5 1,185,078 - Pf1N1B4_1049 0.13 -0.5 1,185,207 + Pf1N1B4_1049 0.24 -0.9 1,185,208 - Pf1N1B4_1049 0.24 -0.3 1,185,208 - Pf1N1B4_1049 0.24 -0.1 1,185,306 + Pf1N1B4_1049 0.32 +1.6 1,185,306 + Pf1N1B4_1049 0.32 +0.9 1,185,306 + Pf1N1B4_1049 0.32 -0.5 1,185,306 + +0.1 1,185,307 - Pf1N1B4_1049 0.32 +0.1 1,185,307 - Pf1N1B4_1049 0.32 +0.1 1,185,354 + Pf1N1B4_1049 0.36 -1.9 1,185,354 + Pf1N1B4_1049 0.36 -0.8 1,185,540 - Pf1N1B4_1049 0.52 +0.1 1,185,540 - Pf1N1B4_1049 0.52 +0.7 1,185,557 - Pf1N1B4_1049 0.54 -2.7 1,185,557 - Pf1N1B4_1049 0.54 -0.9 1,185,625 - Pf1N1B4_1049 0.60 -0.3 1,185,625 - Pf1N1B4_1049 0.60 -2.7 1,185,748 - Pf1N1B4_1049 0.70 +0.1 1,185,843 + Pf1N1B4_1049 0.78 -0.9
Or see this region's nucleotide sequence