Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1018 and Pf1N1B4_1019 are separated by 28 nucleotides Pf1N1B4_1019 and Pf1N1B4_1020 overlap by 8 nucleotides
Pf1N1B4_1018 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 1,153,287 to 1,154,396
_1018
Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414
_1019
Pf1N1B4_1020 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 1,155,407 to 1,156,405
_1020
Position (kb)
1154
1155
1156 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1153.753 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1154.173 kb on + strand, within Pf1N1B4_1018 at 1154.245 kb on + strand, within Pf1N1B4_1018 at 1154.389 kb on + strand at 1154.389 kb on + strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.432 kb on + strand at 1154.433 kb on - strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.504 kb on + strand at 1154.577 kb on - strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.880 kb on - strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.311 kb on - strand, within Pf1N1B4_1019 at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.495 kb on + strand at 1155.588 kb on - strand, within Pf1N1B4_1020 at 1155.631 kb on + strand, within Pf1N1B4_1020 at 1156.157 kb on - strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.179 kb on + strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.180 kb on - strand, within Pf1N1B4_1020 at 1156.239 kb on + strand, within Pf1N1B4_1020 at 1156.240 kb on - strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.250 kb on + strand, within Pf1N1B4_1020 at 1156.251 kb on - strand, within Pf1N1B4_1020 at 1156.251 kb on - strand at 1156.251 kb on - strand, within Pf1N1B4_1020
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,153,753 - Pf1N1B4_1018 0.42 -1.2 1,153,900 - Pf1N1B4_1018 0.55 -0.6 1,153,900 - Pf1N1B4_1018 0.55 +0.1 1,153,900 - Pf1N1B4_1018 0.55 -0.4 1,153,900 - Pf1N1B4_1018 0.55 -1.3 1,154,173 + Pf1N1B4_1018 0.80 -1.2 1,154,245 + Pf1N1B4_1018 0.86 +0.9 1,154,389 + +0.4 1,154,389 + -0.8 1,154,390 - -2.5 1,154,390 - -0.9 1,154,390 - -0.5 1,154,432 + -0.4 1,154,433 - -0.2 1,154,486 + +0.1 1,154,486 + +0.1 1,154,486 + +0.3 1,154,486 + -0.2 1,154,486 + -0.8 1,154,486 + -1.5 1,154,486 + -0.1 1,154,486 + +0.1 1,154,486 + +0.5 1,154,486 + +0.6 1,154,486 + +0.6 1,154,486 + +1.1 1,154,487 - +0.6 1,154,487 - +1.0 1,154,487 - +1.5 1,154,487 - +0.1 1,154,487 - -2.2 1,154,504 + +0.3 1,154,577 - Pf1N1B4_1019 0.15 -0.7 1,154,793 + Pf1N1B4_1019 0.37 -0.7 1,154,793 + Pf1N1B4_1019 0.37 -0.3 1,154,793 + Pf1N1B4_1019 0.37 -0.9 1,154,794 - Pf1N1B4_1019 0.37 +0.1 1,154,794 - Pf1N1B4_1019 0.37 -1.4 1,154,794 - Pf1N1B4_1019 0.37 +0.5 1,154,880 - Pf1N1B4_1019 0.46 +1.1 1,154,902 + Pf1N1B4_1019 0.48 -0.6 1,154,902 + Pf1N1B4_1019 0.48 -2.2 1,154,903 - Pf1N1B4_1019 0.48 +0.1 1,154,903 - Pf1N1B4_1019 0.48 +0.1 1,155,146 + Pf1N1B4_1019 0.73 +2.7 1,155,146 + Pf1N1B4_1019 0.73 -1.2 1,155,146 + Pf1N1B4_1019 0.73 -0.9 1,155,146 + Pf1N1B4_1019 0.73 -0.6 1,155,147 - Pf1N1B4_1019 0.73 -1.7 1,155,147 - Pf1N1B4_1019 0.73 +0.4 1,155,147 - +1.7 1,155,147 - Pf1N1B4_1019 0.73 -0.3 1,155,311 - Pf1N1B4_1019 0.89 +0.1 1,155,495 + -0.2 1,155,495 + +0.1 1,155,495 + -0.5 1,155,588 - Pf1N1B4_1020 0.18 -0.6 1,155,631 + Pf1N1B4_1020 0.22 -1.2 1,156,157 - Pf1N1B4_1020 0.75 +0.1 1,156,179 + Pf1N1B4_1020 0.77 +1.1 1,156,179 + Pf1N1B4_1020 0.77 -1.1 1,156,180 - Pf1N1B4_1020 0.77 -1.2 1,156,180 - Pf1N1B4_1020 0.77 +1.1 1,156,180 - Pf1N1B4_1020 0.77 +0.5 1,156,239 + Pf1N1B4_1020 0.83 +1.1 1,156,240 - Pf1N1B4_1020 0.83 +0.1 1,156,250 + Pf1N1B4_1020 0.84 +0.3 1,156,250 + Pf1N1B4_1020 0.84 +0.1 1,156,250 + Pf1N1B4_1020 0.84 -1.2 1,156,250 + Pf1N1B4_1020 0.84 +0.1 1,156,251 - Pf1N1B4_1020 0.84 +0.3 1,156,251 - +0.1 1,156,251 - Pf1N1B4_1020 0.84 +0.6
Or see this region's nucleotide sequence