Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07540 and MMP_RS07545 are separated by 19 nucleotides MMP_RS07545 and MMP_RS07550 are separated by 13 nucleotides MMP_RS07550 and MMP_RS07555 are separated by 54 nucleotides MMP_RS07555 and MMP_RS07560 are separated by 173 nucleotides
MMP_RS07540: MMP_RS07540 - CBS domain-containing protein, at 1,427,680 to 1,428,321
_RS07540
MMP_RS07545: MMP_RS07545 - HTH domain-containing protein, at 1,428,341 to 1,428,736
_RS07545
MMP_RS07550: MMP_RS07550 - L-serine ammonia-lyase iron-sulfur-dependent subunit alpha, at 1,428,750 to 1,429,940
_RS07550
MMP_RS07555: MMP_RS07555 - monovalent cation/H+ antiporter subunit E, at 1,429,995 to 1,430,315
_RS07555
MMP_RS07560: MMP_RS07560 - prefoldin subunit alpha, at 1,430,489 to 1,430,923
_RS07560
Position (kb)
1428
1429
1430 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1427.762 kb on + strand, within MMP_RS07540 at 1427.898 kb on + strand, within MMP_RS07540 at 1427.940 kb on - strand, within MMP_RS07540 at 1427.953 kb on - strand, within MMP_RS07540 at 1427.968 kb on + strand, within MMP_RS07540 at 1428.202 kb on + strand, within MMP_RS07540 at 1428.358 kb on - strand at 1428.364 kb on - strand at 1428.483 kb on + strand, within MMP_RS07545 at 1428.566 kb on - strand, within MMP_RS07545 at 1428.777 kb on - strand at 1428.876 kb on - strand, within MMP_RS07550 at 1428.958 kb on + strand, within MMP_RS07550 at 1429.071 kb on - strand, within MMP_RS07550 at 1429.107 kb on + strand, within MMP_RS07550 at 1429.299 kb on + strand, within MMP_RS07550 at 1429.307 kb on - strand, within MMP_RS07550 at 1429.386 kb on - strand, within MMP_RS07550 at 1429.387 kb on + strand, within MMP_RS07550 at 1429.418 kb on - strand, within MMP_RS07550 at 1429.418 kb on - strand, within MMP_RS07550 at 1429.418 kb on - strand, within MMP_RS07550 at 1429.419 kb on + strand, within MMP_RS07550 at 1429.419 kb on + strand, within MMP_RS07550 at 1429.420 kb on - strand, within MMP_RS07550 at 1429.421 kb on + strand, within MMP_RS07550 at 1429.494 kb on + strand, within MMP_RS07550 at 1429.641 kb on - strand, within MMP_RS07550 at 1429.661 kb on - strand, within MMP_RS07550 at 1429.714 kb on + strand, within MMP_RS07550 at 1429.743 kb on + strand, within MMP_RS07550 at 1429.784 kb on - strand, within MMP_RS07550 at 1429.917 kb on - strand at 1429.940 kb on - strand at 1429.978 kb on + strand at 1430.013 kb on - strand at 1430.017 kb on - strand at 1430.018 kb on + strand at 1430.068 kb on + strand, within MMP_RS07555 at 1430.350 kb on - strand at 1430.354 kb on - strand at 1430.355 kb on + strand at 1430.356 kb on - strand at 1430.356 kb on - strand at 1430.358 kb on - strand at 1430.359 kb on + strand at 1430.359 kb on + strand at 1430.359 kb on + strand at 1430.360 kb on - strand at 1430.360 kb on - strand at 1430.361 kb on + strand at 1430.361 kb on + strand at 1430.364 kb on - strand at 1430.452 kb on - strand at 1430.453 kb on + strand at 1430.456 kb on - strand at 1430.456 kb on - strand at 1430.555 kb on + strand, within MMP_RS07560 at 1430.745 kb on - strand, within MMP_RS07560 at 1430.746 kb on + strand, within MMP_RS07560 at 1430.746 kb on + strand, within MMP_RS07560 at 1430.809 kb on + strand, within MMP_RS07560 at 1430.809 kb on + strand, within MMP_RS07560 at 1430.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 1,427,762 + MMP_RS07540 0.13 -1.0 1,427,898 + MMP_RS07540 0.34 -1.2 1,427,940 - MMP_RS07540 0.40 -1.5 1,427,953 - MMP_RS07540 0.43 -3.9 1,427,968 + MMP_RS07540 0.45 -0.2 1,428,202 + MMP_RS07540 0.81 -0.5 1,428,358 - -4.7 1,428,364 - -1.6 1,428,483 + MMP_RS07545 0.36 -1.2 1,428,566 - MMP_RS07545 0.57 -2.3 1,428,777 - -0.3 1,428,876 - MMP_RS07550 0.11 -1.0 1,428,958 + MMP_RS07550 0.17 -0.7 1,429,071 - MMP_RS07550 0.27 -0.6 1,429,107 + MMP_RS07550 0.30 -1.3 1,429,299 + MMP_RS07550 0.46 -1.7 1,429,307 - MMP_RS07550 0.47 -0.6 1,429,386 - MMP_RS07550 0.53 -0.9 1,429,387 + MMP_RS07550 0.53 -1.4 1,429,418 - MMP_RS07550 0.56 -1.2 1,429,418 - MMP_RS07550 0.56 -0.7 1,429,418 - MMP_RS07550 0.56 -0.8 1,429,419 + MMP_RS07550 0.56 -2.6 1,429,419 + MMP_RS07550 0.56 -0.4 1,429,420 - MMP_RS07550 0.56 -0.8 1,429,421 + MMP_RS07550 0.56 -2.0 1,429,494 + MMP_RS07550 0.62 -1.0 1,429,641 - MMP_RS07550 0.75 -1.3 1,429,661 - MMP_RS07550 0.76 -1.2 1,429,714 + MMP_RS07550 0.81 -1.3 1,429,743 + MMP_RS07550 0.83 -1.0 1,429,784 - MMP_RS07550 0.87 -0.8 1,429,917 - -0.6 1,429,940 - -1.2 1,429,978 + -2.4 1,430,013 - +0.8 1,430,017 - +1.0 1,430,018 + -1.6 1,430,068 + MMP_RS07555 0.23 -1.3 1,430,350 - -0.6 1,430,354 - -0.7 1,430,355 + -0.1 1,430,356 - +0.4 1,430,356 - -0.4 1,430,358 - +0.6 1,430,359 + +0.1 1,430,359 + -0.8 1,430,359 + -0.8 1,430,360 - +1.2 1,430,360 - +1.0 1,430,361 + +0.7 1,430,361 + +0.5 1,430,364 - -0.5 1,430,452 - +0.5 1,430,453 + -0.3 1,430,456 - +0.8 1,430,456 - +0.6 1,430,555 + MMP_RS07560 0.15 +1.0 1,430,745 - MMP_RS07560 0.59 +0.3 1,430,746 + MMP_RS07560 0.59 +0.0 1,430,746 + MMP_RS07560 0.59 -0.2 1,430,809 + MMP_RS07560 0.74 +0.2 1,430,809 + MMP_RS07560 0.74 +0.5 1,430,921 - +0.7
Or see this region's nucleotide sequence