Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02950 and MMP_RS02955 are separated by 118 nucleotides MMP_RS02955 and MMP_RS02960 are separated by 61 nucleotides MMP_RS02960 and MMP_RS02965 are separated by 13 nucleotides MMP_RS02965 and MMP_RS02970 are separated by 334 nucleotides
MMP_RS02950: MMP_RS02950 - hypothetical protein, at 560,281 to 560,577
_RS02950
MMP_RS02955: MMP_RS02955 - DJ-1/PfpI family protein, at 560,696 to 561,211
_RS02955
MMP_RS02960: MMP_RS02960 - class I SAM-dependent methyltransferase family protein, at 561,273 to 562,034
_RS02960
MMP_RS02965: MMP_RS02965 - carboxymuconolactone decarboxylase family protein, at 562,048 to 562,482
_RS02965
MMP_RS02970: MMP_RS02970 - hypothetical protein, at 562,817 to 563,107
_RS02970
Position (kb) 561
562
563 Strain fitness (log2 ratio) -1
0
1
2 at 560.282 kb on - strand at 560.604 kb on - strand at 560.616 kb on - strand at 560.627 kb on - strand at 560.636 kb on - strand at 560.647 kb on + strand at 560.651 kb on - strand at 560.659 kb on + strand at 560.659 kb on + strand at 560.659 kb on + strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.660 kb on - strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.661 kb on + strand at 560.707 kb on + strand at 560.757 kb on - strand, within MMP_RS02955 at 560.764 kb on + strand, within MMP_RS02955 at 560.896 kb on - strand, within MMP_RS02955 at 560.897 kb on + strand, within MMP_RS02955 at 561.250 kb on - strand at 561.251 kb on + strand at 561.251 kb on + strand at 561.323 kb on - strand at 561.334 kb on - strand at 561.355 kb on + strand, within MMP_RS02960 at 561.366 kb on + strand, within MMP_RS02960 at 561.398 kb on - strand, within MMP_RS02960 at 561.398 kb on - strand, within MMP_RS02960 at 561.399 kb on + strand, within MMP_RS02960 at 561.399 kb on + strand, within MMP_RS02960 at 561.399 kb on + strand, within MMP_RS02960 at 561.402 kb on - strand, within MMP_RS02960 at 561.402 kb on - strand, within MMP_RS02960 at 561.402 kb on - strand, within MMP_RS02960 at 561.651 kb on - strand, within MMP_RS02960 at 561.835 kb on - strand, within MMP_RS02960 at 561.888 kb on + strand, within MMP_RS02960 at 561.968 kb on - strand at 561.982 kb on + strand at 562.111 kb on + strand, within MMP_RS02965 at 562.111 kb on + strand, within MMP_RS02965 at 562.114 kb on + strand, within MMP_RS02965 at 562.210 kb on - strand, within MMP_RS02965 at 562.483 kb on + strand at 562.509 kb on - strand at 562.515 kb on + strand at 562.559 kb on - strand at 562.567 kb on + strand at 562.568 kb on - strand at 562.568 kb on - strand at 562.568 kb on - strand at 562.576 kb on - strand at 562.577 kb on + strand at 562.584 kb on + strand at 562.622 kb on + strand at 562.634 kb on + strand at 562.683 kb on - strand at 562.860 kb on - strand, within MMP_RS02970 at 562.860 kb on - strand, within MMP_RS02970 at 562.918 kb on + strand, within MMP_RS02970 at 562.918 kb on + strand, within MMP_RS02970 at 562.921 kb on + strand, within MMP_RS02970
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE remove 560,282 - -0.3 560,604 - +0.2 560,616 - -0.1 560,627 - -0.3 560,636 - +0.4 560,647 + -0.9 560,651 - -0.0 560,659 + +0.6 560,659 + +0.3 560,659 + -0.7 560,660 - -0.1 560,660 - +0.1 560,660 - +0.2 560,660 - -1.4 560,661 + -0.2 560,661 + -0.1 560,661 + +0.1 560,661 + +0.3 560,661 + -0.5 560,661 + +0.3 560,707 + +0.4 560,757 - MMP_RS02955 0.12 +0.7 560,764 + MMP_RS02955 0.13 +0.1 560,896 - MMP_RS02955 0.39 -1.0 560,897 + MMP_RS02955 0.39 +0.1 561,250 - +0.3 561,251 + -1.4 561,251 + +0.2 561,323 - +1.1 561,334 - +1.4 561,355 + MMP_RS02960 0.11 +1.2 561,366 + MMP_RS02960 0.12 +1.5 561,398 - MMP_RS02960 0.16 +0.8 561,398 - MMP_RS02960 0.16 +1.6 561,399 + MMP_RS02960 0.17 -1.1 561,399 + MMP_RS02960 0.17 +1.4 561,399 + MMP_RS02960 0.17 +1.4 561,402 - MMP_RS02960 0.17 +1.7 561,402 - MMP_RS02960 0.17 +1.5 561,402 - MMP_RS02960 0.17 +1.2 561,651 - MMP_RS02960 0.50 +2.1 561,835 - MMP_RS02960 0.74 +1.3 561,888 + MMP_RS02960 0.81 +1.0 561,968 - +1.3 561,982 + +0.8 562,111 + MMP_RS02965 0.14 -0.0 562,111 + MMP_RS02965 0.14 +0.1 562,114 + MMP_RS02965 0.15 -0.4 562,210 - MMP_RS02965 0.37 -0.2 562,483 + -0.0 562,509 - +0.2 562,515 + -0.4 562,559 - +0.4 562,567 + +0.3 562,568 - +0.2 562,568 - +0.1 562,568 - -1.0 562,576 - -0.1 562,577 + -0.3 562,584 + +0.6 562,622 + -0.3 562,634 + +0.2 562,683 - +0.7 562,860 - MMP_RS02970 0.15 +0.3 562,860 - MMP_RS02970 0.15 +0.0 562,918 + MMP_RS02970 0.35 -0.1 562,918 + MMP_RS02970 0.35 -0.4 562,921 + MMP_RS02970 0.36 +0.3
Or see this region's nucleotide sequence