Experiment: R2A with nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS07455 and LRK54_RS07460 overlap by 14 nucleotides LRK54_RS07460 and LRK54_RS07465 are separated by 238 nucleotides LRK54_RS07465 and LRK54_RS07470 overlap by 4 nucleotides LRK54_RS07470 and LRK54_RS07475 are separated by 32 nucleotides
LRK54_RS07455: LRK54_RS07455 - hypothetical protein, at 1,607,201 to 1,607,989
_RS07455
LRK54_RS07460: LRK54_RS07460 - hypothetical protein, at 1,607,976 to 1,608,314
_RS07460
LRK54_RS07465: LRK54_RS07465 - succinate dehydrogenase, cytochrome b556 subunit, at 1,608,553 to 1,609,089
_RS07465
LRK54_RS07470: LRK54_RS07470 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 1,609,086 to 1,609,493
_RS07470
LRK54_RS07475: LRK54_RS07475 - succinate dehydrogenase flavoprotein subunit, at 1,609,526 to 1,611,322
_RS07475
Position (kb)
1608
1609
1610 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1607.679 kb on + strand, within LRK54_RS07455 at 1607.680 kb on - strand, within LRK54_RS07455 at 1607.804 kb on + strand, within LRK54_RS07455 at 1607.805 kb on - strand, within LRK54_RS07455 at 1607.805 kb on - strand, within LRK54_RS07455 at 1607.805 kb on - strand, within LRK54_RS07455 at 1607.886 kb on + strand, within LRK54_RS07455 at 1607.886 kb on + strand, within LRK54_RS07455 at 1607.886 kb on + strand, within LRK54_RS07455 at 1607.947 kb on + strand at 1607.948 kb on - strand at 1607.948 kb on - strand at 1607.948 kb on - strand at 1607.983 kb on + strand at 1607.983 kb on + strand at 1607.983 kb on + strand at 1608.160 kb on - strand, within LRK54_RS07460 at 1608.160 kb on - strand, within LRK54_RS07460 at 1608.238 kb on + strand, within LRK54_RS07460 at 1608.347 kb on - strand at 1608.347 kb on - strand at 1608.347 kb on - strand at 1608.347 kb on - strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.353 kb on + strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.354 kb on - strand at 1608.413 kb on + strand at 1608.414 kb on - strand at 1608.474 kb on + strand at 1608.474 kb on + strand at 1608.474 kb on + strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.475 kb on - strand at 1608.624 kb on + strand, within LRK54_RS07465 at 1608.624 kb on + strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.625 kb on - strand, within LRK54_RS07465 at 1608.703 kb on + strand, within LRK54_RS07465 at 1608.703 kb on + strand, within LRK54_RS07465 at 1608.703 kb on + strand, within LRK54_RS07465 at 1608.703 kb on + strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.704 kb on - strand, within LRK54_RS07465 at 1608.779 kb on + strand, within LRK54_RS07465 at 1608.779 kb on + strand, within LRK54_RS07465 at 1608.780 kb on - strand, within LRK54_RS07465 at 1608.780 kb on - strand, within LRK54_RS07465 at 1608.780 kb on - strand, within LRK54_RS07465 at 1608.949 kb on + strand, within LRK54_RS07465 at 1608.950 kb on - strand, within LRK54_RS07465 at 1608.950 kb on - strand, within LRK54_RS07465 at 1608.991 kb on + strand, within LRK54_RS07465 at 1608.991 kb on + strand, within LRK54_RS07465 at 1608.991 kb on + strand, within LRK54_RS07465 at 1608.991 kb on + strand, within LRK54_RS07465 at 1608.992 kb on - strand, within LRK54_RS07465 at 1609.040 kb on + strand at 1609.040 kb on + strand at 1609.040 kb on + strand at 1609.249 kb on + strand, within LRK54_RS07470 at 1609.249 kb on + strand, within LRK54_RS07470 at 1609.249 kb on + strand, within LRK54_RS07470 at 1609.249 kb on + strand, within LRK54_RS07470 at 1609.249 kb on + strand, within LRK54_RS07470 at 1609.250 kb on - strand, within LRK54_RS07470 at 1609.250 kb on - strand, within LRK54_RS07470 at 1609.250 kb on - strand, within LRK54_RS07470 at 1609.260 kb on + strand, within LRK54_RS07470 at 1609.260 kb on + strand, within LRK54_RS07470 at 1609.260 kb on + strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.261 kb on - strand, within LRK54_RS07470 at 1609.332 kb on + strand, within LRK54_RS07470 at 1609.333 kb on - strand, within LRK54_RS07470 at 1609.333 kb on - strand, within LRK54_RS07470 at 1609.360 kb on + strand, within LRK54_RS07470 at 1609.360 kb on + strand, within LRK54_RS07470 at 1609.360 kb on + strand, within LRK54_RS07470 at 1609.360 kb on + strand, within LRK54_RS07470 at 1609.361 kb on - strand, within LRK54_RS07470 at 1609.361 kb on - strand, within LRK54_RS07470 at 1609.361 kb on - strand, within LRK54_RS07470 at 1609.374 kb on + strand, within LRK54_RS07470 at 1609.374 kb on + strand, within LRK54_RS07470 at 1609.374 kb on + strand, within LRK54_RS07470 at 1609.374 kb on + strand, within LRK54_RS07470 at 1609.375 kb on - strand, within LRK54_RS07470 at 1609.375 kb on - strand, within LRK54_RS07470 at 1609.375 kb on - strand, within LRK54_RS07470 at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.541 kb on + strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.542 kb on - strand at 1609.562 kb on + strand at 1609.562 kb on + strand at 1609.562 kb on + strand at 1609.562 kb on + strand at 1609.562 kb on + strand at 1609.563 kb on - strand at 1609.606 kb on - strand at 1609.648 kb on - strand at 1609.648 kb on - strand at 1609.675 kb on + strand at 1609.675 kb on + strand at 1609.675 kb on + strand at 1609.675 kb on + strand at 1609.675 kb on + strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.676 kb on - strand at 1609.739 kb on + strand, within LRK54_RS07475 at 1609.739 kb on + strand, within LRK54_RS07475 at 1609.739 kb on + strand, within LRK54_RS07475 at 1609.739 kb on + strand, within LRK54_RS07475 at 1609.739 kb on + strand, within LRK54_RS07475 at 1609.740 kb on - strand, within LRK54_RS07475 at 1609.748 kb on + strand, within LRK54_RS07475 at 1609.748 kb on + strand, within LRK54_RS07475 at 1609.748 kb on + strand, within LRK54_RS07475 at 1609.748 kb on + strand, within LRK54_RS07475 at 1609.748 kb on + strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.749 kb on - strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.799 kb on + strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.800 kb on - strand, within LRK54_RS07475 at 1609.883 kb on + strand, within LRK54_RS07475 at 1609.884 kb on - strand, within LRK54_RS07475 at 1609.884 kb on - strand, within LRK54_RS07475 at 1609.884 kb on - strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.988 kb on + strand, within LRK54_RS07475 at 1609.989 kb on - strand, within LRK54_RS07475 at 1609.989 kb on - strand, within LRK54_RS07475 at 1609.989 kb on - strand, within LRK54_RS07475 at 1609.989 kb on - strand, within LRK54_RS07475 at 1610.031 kb on - strand, within LRK54_RS07475 at 1610.031 kb on - strand, within LRK54_RS07475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 250 mM remove 1,607,679 + LRK54_RS07455 0.61 +0.3 1,607,680 - LRK54_RS07455 0.61 +0.5 1,607,804 + LRK54_RS07455 0.76 +0.3 1,607,805 - LRK54_RS07455 0.77 -0.4 1,607,805 - LRK54_RS07455 0.77 -0.3 1,607,805 - LRK54_RS07455 0.77 -1.0 1,607,886 + LRK54_RS07455 0.87 +0.6 1,607,886 + LRK54_RS07455 0.87 +0.3 1,607,886 + LRK54_RS07455 0.87 -0.8 1,607,947 + -2.1 1,607,948 - +1.4 1,607,948 - +0.5 1,607,948 - -2.0 1,607,983 + +0.5 1,607,983 + +0.0 1,607,983 + +0.4 1,608,160 - LRK54_RS07460 0.54 -0.9 1,608,160 - LRK54_RS07460 0.54 -0.1 1,608,238 + LRK54_RS07460 0.77 +0.5 1,608,347 - -0.1 1,608,347 - +0.3 1,608,347 - +0.5 1,608,347 - -0.5 1,608,353 + -0.2 1,608,353 + +0.1 1,608,353 + +0.3 1,608,353 + -0.6 1,608,353 + -1.0 1,608,353 + -0.8 1,608,353 + -1.4 1,608,353 + +0.3 1,608,354 - -0.0 1,608,354 - -0.9 1,608,354 - -0.3 1,608,354 - -0.7 1,608,354 - -0.7 1,608,354 - +0.2 1,608,354 - +0.2 1,608,354 - +0.3 1,608,354 - +0.4 1,608,413 + -0.5 1,608,414 - -0.2 1,608,474 + -2.0 1,608,474 + -1.1 1,608,474 + -1.2 1,608,475 - +0.4 1,608,475 - -1.9 1,608,475 - -1.1 1,608,475 - -1.0 1,608,475 - -0.8 1,608,475 - +1.5 1,608,475 - -2.2 1,608,624 + LRK54_RS07465 0.13 -0.5 1,608,624 + LRK54_RS07465 0.13 -1.6 1,608,625 - LRK54_RS07465 0.13 -1.3 1,608,625 - LRK54_RS07465 0.13 -0.5 1,608,625 - LRK54_RS07465 0.13 -1.1 1,608,625 - LRK54_RS07465 0.13 -0.4 1,608,625 - LRK54_RS07465 0.13 +1.4 1,608,625 - LRK54_RS07465 0.13 +2.5 1,608,625 - LRK54_RS07465 0.13 +0.4 1,608,703 + LRK54_RS07465 0.28 -0.2 1,608,703 + LRK54_RS07465 0.28 -0.2 1,608,703 + LRK54_RS07465 0.28 -0.8 1,608,703 + LRK54_RS07465 0.28 -2.4 1,608,704 - LRK54_RS07465 0.28 +0.1 1,608,704 - LRK54_RS07465 0.28 -1.3 1,608,704 - LRK54_RS07465 0.28 -0.7 1,608,704 - LRK54_RS07465 0.28 -0.3 1,608,704 - LRK54_RS07465 0.28 -0.7 1,608,704 - LRK54_RS07465 0.28 -0.6 1,608,779 + LRK54_RS07465 0.42 +0.2 1,608,779 + LRK54_RS07465 0.42 -0.7 1,608,780 - LRK54_RS07465 0.42 -0.6 1,608,780 - LRK54_RS07465 0.42 -0.7 1,608,780 - LRK54_RS07465 0.42 -0.3 1,608,949 + LRK54_RS07465 0.74 -1.5 1,608,950 - LRK54_RS07465 0.74 -1.3 1,608,950 - LRK54_RS07465 0.74 +0.2 1,608,991 + LRK54_RS07465 0.82 -1.1 1,608,991 + LRK54_RS07465 0.82 -1.4 1,608,991 + LRK54_RS07465 0.82 -2.5 1,608,991 + LRK54_RS07465 0.82 +0.6 1,608,992 - LRK54_RS07465 0.82 -1.0 1,609,040 + -1.1 1,609,040 + +1.1 1,609,040 + -1.7 1,609,249 + LRK54_RS07470 0.40 +1.5 1,609,249 + LRK54_RS07470 0.40 -1.0 1,609,249 + LRK54_RS07470 0.40 -0.0 1,609,249 + LRK54_RS07470 0.40 +0.5 1,609,249 + LRK54_RS07470 0.40 -0.7 1,609,250 - LRK54_RS07470 0.40 +0.3 1,609,250 - LRK54_RS07470 0.40 +1.2 1,609,250 - LRK54_RS07470 0.40 -0.2 1,609,260 + LRK54_RS07470 0.43 -0.5 1,609,260 + LRK54_RS07470 0.43 +0.8 1,609,260 + LRK54_RS07470 0.43 -0.2 1,609,261 - LRK54_RS07470 0.43 -0.9 1,609,261 - LRK54_RS07470 0.43 -1.0 1,609,261 - LRK54_RS07470 0.43 +0.7 1,609,261 - LRK54_RS07470 0.43 -1.0 1,609,261 - LRK54_RS07470 0.43 +0.1 1,609,261 - LRK54_RS07470 0.43 +0.1 1,609,332 + LRK54_RS07470 0.60 +0.2 1,609,333 - LRK54_RS07470 0.61 -0.4 1,609,333 - LRK54_RS07470 0.61 -1.1 1,609,360 + LRK54_RS07470 0.67 +0.7 1,609,360 + LRK54_RS07470 0.67 -0.7 1,609,360 + LRK54_RS07470 0.67 -0.4 1,609,360 + LRK54_RS07470 0.67 -0.5 1,609,361 - LRK54_RS07470 0.67 -1.2 1,609,361 - LRK54_RS07470 0.67 +0.1 1,609,361 - LRK54_RS07470 0.67 -0.7 1,609,374 + LRK54_RS07470 0.71 +2.3 1,609,374 + LRK54_RS07470 0.71 -0.5 1,609,374 + LRK54_RS07470 0.71 +1.5 1,609,374 + LRK54_RS07470 0.71 -0.2 1,609,375 - LRK54_RS07470 0.71 -0.2 1,609,375 - LRK54_RS07470 0.71 +1.0 1,609,375 - LRK54_RS07470 0.71 -1.0 1,609,541 + -0.6 1,609,541 + -1.5 1,609,541 + +0.4 1,609,541 + -0.5 1,609,541 + +1.2 1,609,541 + +0.8 1,609,541 + -1.0 1,609,541 + -0.4 1,609,541 + +0.4 1,609,541 + -1.1 1,609,542 - -1.2 1,609,542 - +1.5 1,609,542 - +0.5 1,609,542 - +0.8 1,609,542 - -1.3 1,609,542 - -1.5 1,609,542 - -0.7 1,609,542 - -0.1 1,609,542 - -0.1 1,609,542 - -0.9 1,609,542 - +0.5 1,609,562 + -0.2 1,609,562 + -0.4 1,609,562 + +1.5 1,609,562 + -0.8 1,609,562 + -0.7 1,609,563 - +0.5 1,609,606 - +0.5 1,609,648 - +0.2 1,609,648 - -1.3 1,609,675 + -0.5 1,609,675 + +0.5 1,609,675 + +0.9 1,609,675 + -1.1 1,609,675 + +0.1 1,609,676 - +0.2 1,609,676 - +0.8 1,609,676 - -0.4 1,609,676 - -1.1 1,609,676 - +0.7 1,609,676 - -0.0 1,609,676 - -0.7 1,609,676 - -0.4 1,609,676 - +0.7 1,609,676 - -0.2 1,609,739 + LRK54_RS07475 0.12 +0.0 1,609,739 + LRK54_RS07475 0.12 +0.6 1,609,739 + LRK54_RS07475 0.12 -1.3 1,609,739 + LRK54_RS07475 0.12 -0.5 1,609,739 + LRK54_RS07475 0.12 +0.9 1,609,740 - LRK54_RS07475 0.12 -0.5 1,609,748 + LRK54_RS07475 0.12 +1.0 1,609,748 + LRK54_RS07475 0.12 -0.5 1,609,748 + LRK54_RS07475 0.12 -0.5 1,609,748 + LRK54_RS07475 0.12 +0.4 1,609,748 + LRK54_RS07475 0.12 -1.0 1,609,749 - LRK54_RS07475 0.12 +0.9 1,609,749 - LRK54_RS07475 0.12 +0.5 1,609,749 - LRK54_RS07475 0.12 +1.0 1,609,749 - LRK54_RS07475 0.12 -0.3 1,609,749 - LRK54_RS07475 0.12 +0.6 1,609,749 - LRK54_RS07475 0.12 -0.0 1,609,799 + LRK54_RS07475 0.15 -0.7 1,609,799 + LRK54_RS07475 0.15 -1.4 1,609,799 + LRK54_RS07475 0.15 +0.9 1,609,799 + LRK54_RS07475 0.15 +0.4 1,609,799 + LRK54_RS07475 0.15 +0.7 1,609,799 + LRK54_RS07475 0.15 -0.8 1,609,799 + LRK54_RS07475 0.15 +0.3 1,609,799 + LRK54_RS07475 0.15 +0.1 1,609,799 + LRK54_RS07475 0.15 +2.5 1,609,799 + LRK54_RS07475 0.15 -0.2 1,609,799 + LRK54_RS07475 0.15 +1.2 1,609,799 + LRK54_RS07475 0.15 -0.4 1,609,800 - LRK54_RS07475 0.15 -0.9 1,609,800 - LRK54_RS07475 0.15 -1.3 1,609,800 - LRK54_RS07475 0.15 -0.3 1,609,800 - LRK54_RS07475 0.15 -0.8 1,609,800 - LRK54_RS07475 0.15 +0.1 1,609,800 - LRK54_RS07475 0.15 +0.1 1,609,800 - LRK54_RS07475 0.15 -1.1 1,609,800 - LRK54_RS07475 0.15 +0.5 1,609,800 - LRK54_RS07475 0.15 -0.1 1,609,800 - LRK54_RS07475 0.15 -1.0 1,609,800 - LRK54_RS07475 0.15 -0.3 1,609,800 - LRK54_RS07475 0.15 -0.5 1,609,800 - LRK54_RS07475 0.15 +0.5 1,609,800 - LRK54_RS07475 0.15 -0.7 1,609,800 - LRK54_RS07475 0.15 -0.1 1,609,883 + LRK54_RS07475 0.20 -0.1 1,609,884 - LRK54_RS07475 0.20 +1.0 1,609,884 - LRK54_RS07475 0.20 -1.2 1,609,884 - LRK54_RS07475 0.20 +0.4 1,609,988 + LRK54_RS07475 0.26 -0.8 1,609,988 + LRK54_RS07475 0.26 -0.1 1,609,988 + LRK54_RS07475 0.26 +0.1 1,609,988 + LRK54_RS07475 0.26 -1.0 1,609,988 + LRK54_RS07475 0.26 -0.3 1,609,988 + LRK54_RS07475 0.26 -1.2 1,609,989 - LRK54_RS07475 0.26 +0.1 1,609,989 - LRK54_RS07475 0.26 +1.3 1,609,989 - LRK54_RS07475 0.26 -1.0 1,609,989 - LRK54_RS07475 0.26 -0.4 1,610,031 - LRK54_RS07475 0.28 -1.5 1,610,031 - LRK54_RS07475 0.28 -0.6
Or see this region's nucleotide sequence