Experiment: R2A with nitrate 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS01240 and LRK54_RS01245 are separated by 59 nucleotides LRK54_RS01245 and LRK54_RS01250 are separated by 19 nucleotides LRK54_RS01250 and LRK54_RS01255 are separated by 13 nucleotides LRK54_RS01255 and LRK54_RS01260 overlap by 1 nucleotides
LRK54_RS01240: LRK54_RS01240 - IS4 family transposase, at 273,560 to 274,900
_RS01240
LRK54_RS01245: LRK54_RS01245 - histidine triad nucleotide-binding protein, at 274,960 to 275,304
_RS01245
LRK54_RS01250: LRK54_RS01250 - recombination mediator RecR, at 275,324 to 275,923
_RS01250
LRK54_RS01255: LRK54_RS01255 - YbaB/EbfC family nucleoid-associated protein, at 275,937 to 276,260
_RS01255
LRK54_RS01260: LRK54_RS01260 - DNA polymerase III subunit gamma/tau, at 276,260 to 277,945
_RS01260
Position (kb) 275
276 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 274.898 kb on + strand at 274.898 kb on + strand at 274.898 kb on + strand at 274.898 kb on + strand at 274.898 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.912 kb on + strand at 274.935 kb on + strand at 274.936 kb on - strand at 275.015 kb on + strand, within LRK54_RS01245 at 275.015 kb on + strand, within LRK54_RS01245 at 275.015 kb on + strand, within LRK54_RS01245 at 275.015 kb on + strand, within LRK54_RS01245 at 275.015 kb on + strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.016 kb on - strand, within LRK54_RS01245 at 275.093 kb on + strand, within LRK54_RS01245 at 275.093 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.230 kb on + strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.231 kb on - strand, within LRK54_RS01245 at 275.246 kb on + strand, within LRK54_RS01245 at 275.246 kb on + strand, within LRK54_RS01245 at 275.246 kb on + strand, within LRK54_RS01245 at 275.246 kb on + strand, within LRK54_RS01245 at 275.246 kb on + strand, within LRK54_RS01245 at 275.247 kb on - strand, within LRK54_RS01245 at 275.321 kb on + strand at 275.322 kb on - strand at 275.322 kb on - strand at 275.322 kb on - strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.379 kb on + strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.380 kb on - strand at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.460 kb on + strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.461 kb on - strand, within LRK54_RS01250 at 275.613 kb on + strand, within LRK54_RS01250 at 275.613 kb on + strand, within LRK54_RS01250 at 275.613 kb on + strand, within LRK54_RS01250 at 275.613 kb on + strand, within LRK54_RS01250 at 275.613 kb on + strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250 at 275.614 kb on - strand, within LRK54_RS01250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 250 mM remove 274,898 + -1.2 274,898 + +0.9 274,898 + -1.1 274,898 + -0.8 274,898 + -0.0 274,912 + -0.1 274,912 + +0.1 274,912 + -2.2 274,912 + +0.5 274,912 + -0.7 274,912 + +0.1 274,912 + -1.3 274,912 + -0.2 274,912 + -1.9 274,912 + -0.9 274,935 + -0.7 274,936 - +1.6 275,015 + LRK54_RS01245 0.16 -0.2 275,015 + LRK54_RS01245 0.16 -0.3 275,015 + LRK54_RS01245 0.16 -0.2 275,015 + LRK54_RS01245 0.16 -0.8 275,015 + LRK54_RS01245 0.16 +0.9 275,016 - LRK54_RS01245 0.16 -1.0 275,016 - LRK54_RS01245 0.16 +0.1 275,016 - LRK54_RS01245 0.16 -0.9 275,016 - LRK54_RS01245 0.16 -0.4 275,016 - LRK54_RS01245 0.16 +1.2 275,016 - LRK54_RS01245 0.16 +0.6 275,016 - LRK54_RS01245 0.16 -1.7 275,016 - LRK54_RS01245 0.16 +0.5 275,016 - LRK54_RS01245 0.16 -3.2 275,016 - LRK54_RS01245 0.16 +0.2 275,016 - LRK54_RS01245 0.16 +0.4 275,016 - LRK54_RS01245 0.16 -0.2 275,016 - LRK54_RS01245 0.16 -2.0 275,016 - LRK54_RS01245 0.16 -0.7 275,016 - LRK54_RS01245 0.16 -1.5 275,016 - LRK54_RS01245 0.16 +0.3 275,016 - LRK54_RS01245 0.16 -0.4 275,016 - LRK54_RS01245 0.16 -2.9 275,093 + LRK54_RS01245 0.39 -2.4 275,093 + LRK54_RS01245 0.39 +0.3 275,230 + LRK54_RS01245 0.78 -1.8 275,230 + LRK54_RS01245 0.78 +0.9 275,230 + LRK54_RS01245 0.78 -0.1 275,230 + LRK54_RS01245 0.78 +0.9 275,230 + LRK54_RS01245 0.78 +0.6 275,230 + LRK54_RS01245 0.78 -1.4 275,230 + LRK54_RS01245 0.78 -1.5 275,230 + LRK54_RS01245 0.78 +0.7 275,230 + LRK54_RS01245 0.78 +0.7 275,230 + LRK54_RS01245 0.78 +1.4 275,230 + LRK54_RS01245 0.78 +2.2 275,230 + LRK54_RS01245 0.78 -0.1 275,230 + LRK54_RS01245 0.78 -1.9 275,230 + LRK54_RS01245 0.78 -0.1 275,231 - LRK54_RS01245 0.79 -1.4 275,231 - LRK54_RS01245 0.79 -1.0 275,231 - LRK54_RS01245 0.79 -0.3 275,231 - LRK54_RS01245 0.79 +0.3 275,231 - LRK54_RS01245 0.79 -3.0 275,231 - LRK54_RS01245 0.79 +1.7 275,231 - LRK54_RS01245 0.79 -1.6 275,231 - LRK54_RS01245 0.79 -0.7 275,231 - LRK54_RS01245 0.79 -0.7 275,231 - LRK54_RS01245 0.79 -1.6 275,231 - LRK54_RS01245 0.79 +0.1 275,246 + LRK54_RS01245 0.83 +0.8 275,246 + LRK54_RS01245 0.83 +0.3 275,246 + LRK54_RS01245 0.83 +2.5 275,246 + LRK54_RS01245 0.83 -2.9 275,246 + LRK54_RS01245 0.83 -0.9 275,247 - LRK54_RS01245 0.83 +0.9 275,321 + +0.1 275,322 - +0.3 275,322 - +0.1 275,322 - +0.1 275,379 + +1.9 275,379 + +0.1 275,379 + +1.2 275,379 + +0.5 275,379 + -0.3 275,379 + -0.4 275,379 + -1.5 275,379 + -0.1 275,379 + -0.7 275,379 + +0.1 275,379 + +2.0 275,379 + +1.0 275,379 + -0.5 275,379 + +0.1 275,379 + -1.0 275,379 + -0.7 275,379 + -1.7 275,379 + +0.1 275,379 + +0.5 275,380 - +0.3 275,380 - -1.2 275,380 - -0.5 275,380 - -0.9 275,380 - -0.7 275,380 - +2.5 275,380 - +0.1 275,380 - -0.5 275,380 - -0.7 275,380 - -1.3 275,380 - +0.0 275,380 - -0.5 275,460 + LRK54_RS01250 0.23 +1.1 275,460 + LRK54_RS01250 0.23 -1.4 275,460 + LRK54_RS01250 0.23 -0.7 275,460 + LRK54_RS01250 0.23 +1.9 275,460 + LRK54_RS01250 0.23 -1.2 275,460 + LRK54_RS01250 0.23 -1.1 275,460 + LRK54_RS01250 0.23 -0.5 275,460 + LRK54_RS01250 0.23 -0.4 275,460 + LRK54_RS01250 0.23 -1.0 275,460 + LRK54_RS01250 0.23 -1.5 275,460 + LRK54_RS01250 0.23 +0.1 275,460 + LRK54_RS01250 0.23 -1.7 275,460 + LRK54_RS01250 0.23 -0.9 275,460 + LRK54_RS01250 0.23 -1.7 275,460 + LRK54_RS01250 0.23 -1.1 275,460 + LRK54_RS01250 0.23 -0.1 275,460 + LRK54_RS01250 0.23 +0.6 275,461 - LRK54_RS01250 0.23 +1.5 275,461 - LRK54_RS01250 0.23 -0.3 275,461 - LRK54_RS01250 0.23 -0.7 275,461 - LRK54_RS01250 0.23 -0.6 275,461 - LRK54_RS01250 0.23 -1.0 275,461 - LRK54_RS01250 0.23 +0.5 275,461 - LRK54_RS01250 0.23 +1.1 275,461 - LRK54_RS01250 0.23 -1.4 275,461 - LRK54_RS01250 0.23 -1.0 275,461 - LRK54_RS01250 0.23 +0.4 275,461 - LRK54_RS01250 0.23 -2.1 275,461 - LRK54_RS01250 0.23 -0.6 275,613 + LRK54_RS01250 0.48 -0.1 275,613 + LRK54_RS01250 0.48 +0.2 275,613 + LRK54_RS01250 0.48 +1.0 275,613 + LRK54_RS01250 0.48 +0.1 275,613 + LRK54_RS01250 0.48 -1.7 275,614 - LRK54_RS01250 0.48 -0.5 275,614 - LRK54_RS01250 0.48 -0.3 275,614 - LRK54_RS01250 0.48 -0.8 275,614 - LRK54_RS01250 0.48 +0.5 275,614 - LRK54_RS01250 0.48 -1.6 275,614 - LRK54_RS01250 0.48 +0.1 275,614 - LRK54_RS01250 0.48 -0.0 275,614 - LRK54_RS01250 0.48 -0.1 275,614 - LRK54_RS01250 0.48 -0.2 275,614 - LRK54_RS01250 0.48 -0.1 275,614 - LRK54_RS01250 0.48 -1.3 275,614 - LRK54_RS01250 0.48 +0.5
Or see this region's nucleotide sequence