Experiment: Bacitracin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_665 and Ac3H11_666 are separated by 185 nucleotides Ac3H11_666 and Ac3H11_667 are separated by 59 nucleotides Ac3H11_667 and Ac3H11_668 overlap by 4 nucleotides
Ac3H11_665 - Magnesium and cobalt transport protein CorA, at 22,337 to 23,323
_665
Ac3H11_666 - Bacteriophage-related DNA polymerase, at 23,509 to 24,279
_666
Ac3H11_667 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 24,339 to 24,842
_667
Ac3H11_668 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 24,839 to 25,576
_668
Position (kb) 23
24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 23.410 kb on - strand at 23.410 kb on - strand at 23.422 kb on - strand at 23.469 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.280 kb on - strand at 24.340 kb on + strand at 24.395 kb on - strand, within Ac3H11_667 at 24.395 kb on - strand, within Ac3H11_667 at 24.397 kb on + strand, within Ac3H11_667 at 24.398 kb on - strand, within Ac3H11_667 at 24.398 kb on - strand, within Ac3H11_667 at 24.439 kb on + strand, within Ac3H11_667 at 24.439 kb on + strand, within Ac3H11_667 at 24.440 kb on - strand, within Ac3H11_667 at 24.716 kb on - strand, within Ac3H11_667
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.008 mg/ml remove 23,410 - -2.0 23,410 - -0.4 23,422 - +3.8 23,469 - +1.8 24,280 - +1.4 24,280 - +0.6 24,280 - -0.2 24,280 - -0.7 24,340 + +0.9 24,395 - Ac3H11_667 0.11 +0.8 24,395 - Ac3H11_667 0.11 -1.1 24,397 + Ac3H11_667 0.12 -1.0 24,398 - Ac3H11_667 0.12 -0.5 24,398 - Ac3H11_667 0.12 -0.4 24,439 + Ac3H11_667 0.20 +0.8 24,439 + Ac3H11_667 0.20 -1.4 24,440 - Ac3H11_667 0.20 -3.3 24,716 - Ac3H11_667 0.75 -2.0
Or see this region's nucleotide sequence