Experiment: Bacitracin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3396 and Ac3H11_3397 are separated by 44 nucleotides Ac3H11_3397 and Ac3H11_3398 are separated by 54 nucleotides Ac3H11_3398 and Ac3H11_3399 are separated by 48 nucleotides Ac3H11_3399 and Ac3H11_3400 are separated by 32 nucleotides
Ac3H11_3396 - ProQ: influences osmotic activation of compatible solute ProP, at 73,466 to 74,437
_3396
Ac3H11_3397 - Conserved domain protein, at 74,482 to 75,096
_3397
Ac3H11_3398 - Glycolate dehydrogenase (EC 1.1.99.14), iron-sulfur subunit GlcF, at 75,151 to 76,404
_3398
Ac3H11_3399 - Probable transmembrane protein, at 76,453 to 77,250
_3399
Ac3H11_3400 - Glycolate dehydrogenase (EC 1.1.99.14), FAD-binding subunit GlcE, at 77,283 to 78,410
_3400
Position (kb)
75
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 74.483 kb on + strand at 74.484 kb on - strand at 74.484 kb on - strand at 74.484 kb on - strand at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.634 kb on - strand, within Ac3H11_3397 at 74.756 kb on + strand, within Ac3H11_3397 at 74.794 kb on + strand, within Ac3H11_3397 at 74.794 kb on + strand, within Ac3H11_3397 at 74.795 kb on - strand, within Ac3H11_3397 at 74.941 kb on + strand, within Ac3H11_3397 at 74.942 kb on - strand, within Ac3H11_3397 at 75.133 kb on - strand at 75.320 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.347 kb on + strand, within Ac3H11_3398 at 75.348 kb on - strand, within Ac3H11_3398 at 75.479 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.485 kb on + strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.486 kb on - strand, within Ac3H11_3398 at 75.605 kb on + strand, within Ac3H11_3398 at 75.605 kb on + strand, within Ac3H11_3398 at 75.635 kb on + strand, within Ac3H11_3398 at 76.133 kb on + strand, within Ac3H11_3398 at 76.134 kb on - strand, within Ac3H11_3398 at 76.134 kb on - strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.247 kb on + strand, within Ac3H11_3398 at 76.248 kb on - strand, within Ac3H11_3398 at 76.248 kb on - strand, within Ac3H11_3398 at 76.292 kb on + strand at 76.293 kb on - strand at 76.293 kb on - strand at 76.293 kb on - strand at 76.293 kb on - strand at 77.044 kb on + strand, within Ac3H11_3399 at 77.044 kb on + strand, within Ac3H11_3399 at 77.044 kb on + strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.045 kb on - strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.126 kb on + strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.127 kb on - strand, within Ac3H11_3399 at 77.284 kb on + strand at 77.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.008 mg/ml remove 74,483 + +1.4 74,484 - +0.5 74,484 - +0.5 74,484 - +0.5 74,634 - Ac3H11_3397 0.25 -1.0 74,634 - Ac3H11_3397 0.25 +2.2 74,634 - Ac3H11_3397 0.25 -0.7 74,634 - Ac3H11_3397 0.25 -0.2 74,756 + Ac3H11_3397 0.45 -1.4 74,794 + Ac3H11_3397 0.51 +1.3 74,794 + Ac3H11_3397 0.51 +1.6 74,795 - Ac3H11_3397 0.51 -1.1 74,941 + Ac3H11_3397 0.75 -2.3 74,942 - Ac3H11_3397 0.75 -0.4 75,133 - -0.0 75,320 + Ac3H11_3398 0.13 -0.9 75,347 + Ac3H11_3398 0.16 -0.1 75,347 + Ac3H11_3398 0.16 -0.7 75,347 + Ac3H11_3398 0.16 +0.1 75,347 + Ac3H11_3398 0.16 -1.9 75,347 + Ac3H11_3398 0.16 +0.8 75,347 + Ac3H11_3398 0.16 +0.1 75,348 - Ac3H11_3398 0.16 +1.0 75,479 + Ac3H11_3398 0.26 +0.4 75,485 + Ac3H11_3398 0.27 -1.9 75,485 + Ac3H11_3398 0.27 +0.8 75,485 + Ac3H11_3398 0.27 -0.6 75,485 + Ac3H11_3398 0.27 -1.7 75,485 + Ac3H11_3398 0.27 -1.3 75,485 + Ac3H11_3398 0.27 +1.5 75,485 + Ac3H11_3398 0.27 +0.7 75,485 + Ac3H11_3398 0.27 +1.9 75,485 + Ac3H11_3398 0.27 -1.5 75,485 + Ac3H11_3398 0.27 +0.4 75,486 - Ac3H11_3398 0.27 +0.2 75,486 - Ac3H11_3398 0.27 -0.5 75,486 - Ac3H11_3398 0.27 +0.1 75,486 - Ac3H11_3398 0.27 -0.0 75,486 - Ac3H11_3398 0.27 -0.2 75,486 - Ac3H11_3398 0.27 +1.0 75,486 - Ac3H11_3398 0.27 -0.9 75,486 - Ac3H11_3398 0.27 -3.0 75,486 - Ac3H11_3398 0.27 -2.1 75,486 - Ac3H11_3398 0.27 +1.0 75,486 - Ac3H11_3398 0.27 +1.7 75,486 - Ac3H11_3398 0.27 +0.3 75,486 - Ac3H11_3398 0.27 +2.5 75,605 + Ac3H11_3398 0.36 +0.1 75,605 + Ac3H11_3398 0.36 -2.4 75,635 + Ac3H11_3398 0.39 +0.1 76,133 + Ac3H11_3398 0.78 -1.5 76,134 - Ac3H11_3398 0.78 +0.9 76,134 - Ac3H11_3398 0.78 +2.2 76,247 + Ac3H11_3398 0.87 -1.3 76,247 + Ac3H11_3398 0.87 +0.3 76,247 + Ac3H11_3398 0.87 -1.0 76,247 + Ac3H11_3398 0.87 -0.3 76,248 - Ac3H11_3398 0.87 +0.8 76,248 - Ac3H11_3398 0.87 -0.5 76,292 + -0.0 76,293 - -1.2 76,293 - -0.5 76,293 - +0.4 76,293 - +0.5 77,044 + Ac3H11_3399 0.74 +0.2 77,044 + Ac3H11_3399 0.74 +0.5 77,044 + Ac3H11_3399 0.74 -0.0 77,045 - Ac3H11_3399 0.74 +0.9 77,045 - Ac3H11_3399 0.74 +1.4 77,045 - Ac3H11_3399 0.74 +0.5 77,045 - Ac3H11_3399 0.74 +0.2 77,045 - Ac3H11_3399 0.74 +1.1 77,126 + Ac3H11_3399 0.84 -0.9 77,126 + Ac3H11_3399 0.84 -0.0 77,126 + Ac3H11_3399 0.84 +0.9 77,126 + Ac3H11_3399 0.84 +1.1 77,126 + Ac3H11_3399 0.84 +0.1 77,127 - Ac3H11_3399 0.84 -0.4 77,127 - Ac3H11_3399 0.84 -0.1 77,127 - Ac3H11_3399 0.84 +0.7 77,127 - Ac3H11_3399 0.84 -0.1 77,127 - Ac3H11_3399 0.84 -1.3 77,284 + -0.5 77,284 + +0.9
Or see this region's nucleotide sequence