Experiment: Bacitracin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1157 and Ac3H11_1158 are separated by 88 nucleotides Ac3H11_1158 and Ac3H11_1159 are separated by 133 nucleotides Ac3H11_1159 and Ac3H11_1160 overlap by 4 nucleotides Ac3H11_1160 and Ac3H11_1161 are separated by 93 nucleotides Ac3H11_1161 and Ac3H11_1162 overlap by 48 nucleotides
Ac3H11_1157 - Kynureninase (EC 3.7.1.3), at 109,362 to 110,672
_1157
Ac3H11_1158 - Kynurenine formamidase, bacterial (EC 3.5.1.9), at 110,761 to 111,414
_1158
Ac3H11_1159 - Sensory histidine kinase QseC, at 111,548 to 112,969
_1159
Ac3H11_1160 - probable two-component system response regulator, at 112,966 to 113,631
_1160
Ac3H11_1161 - hypothetical protein, at 113,725 to 113,877
_1161
Ac3H11_1162 - putative peptidase, at 113,830 to 115,740
_1162
Position (kb)
111
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 110.578 kb on + strand at 110.578 kb on + strand at 110.579 kb on - strand at 110.710 kb on + strand at 110.762 kb on + strand at 110.762 kb on + strand at 110.762 kb on + strand at 110.762 kb on + strand at 110.763 kb on - strand at 110.763 kb on - strand at 110.763 kb on - strand at 111.012 kb on - strand, within Ac3H11_1158 at 111.056 kb on + strand, within Ac3H11_1158 at 111.056 kb on + strand, within Ac3H11_1158 at 111.056 kb on + strand, within Ac3H11_1158 at 111.057 kb on - strand, within Ac3H11_1158 at 111.057 kb on - strand, within Ac3H11_1158 at 111.057 kb on - strand, within Ac3H11_1158 at 111.057 kb on - strand, within Ac3H11_1158 at 111.057 kb on - strand, within Ac3H11_1158 at 111.215 kb on + strand, within Ac3H11_1158 at 111.215 kb on + strand, within Ac3H11_1158 at 111.216 kb on - strand, within Ac3H11_1158 at 111.216 kb on - strand, within Ac3H11_1158 at 111.216 kb on - strand, within Ac3H11_1158 at 111.320 kb on + strand, within Ac3H11_1158 at 111.320 kb on + strand, within Ac3H11_1158 at 111.491 kb on + strand at 112.400 kb on + strand, within Ac3H11_1159 at 112.400 kb on + strand, within Ac3H11_1159 at 112.400 kb on + strand, within Ac3H11_1159 at 112.400 kb on + strand, within Ac3H11_1159 at 112.400 kb on + strand, within Ac3H11_1159 at 112.401 kb on - strand, within Ac3H11_1159 at 112.401 kb on - strand, within Ac3H11_1159 at 112.425 kb on + strand, within Ac3H11_1159 at 112.449 kb on + strand, within Ac3H11_1159 at 112.449 kb on + strand, within Ac3H11_1159 at 112.521 kb on + strand, within Ac3H11_1159 at 112.521 kb on + strand, within Ac3H11_1159 at 112.522 kb on - strand, within Ac3H11_1159 at 112.522 kb on - strand, within Ac3H11_1159 at 112.522 kb on - strand, within Ac3H11_1159 at 112.628 kb on - strand, within Ac3H11_1159 at 112.830 kb on + strand at 112.830 kb on + strand at 112.830 kb on + strand at 112.831 kb on - strand at 112.845 kb on + strand at 112.982 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.008 mg/ml remove 110,578 + -0.3 110,578 + +0.2 110,579 - -0.0 110,710 + +1.0 110,762 + -0.4 110,762 + -0.5 110,762 + +0.9 110,762 + +0.7 110,763 - +0.2 110,763 - +1.5 110,763 - -0.4 111,012 - Ac3H11_1158 0.38 +0.6 111,056 + Ac3H11_1158 0.45 +0.9 111,056 + Ac3H11_1158 0.45 -1.0 111,056 + Ac3H11_1158 0.45 +1.0 111,057 - Ac3H11_1158 0.45 -0.1 111,057 - Ac3H11_1158 0.45 +0.1 111,057 - Ac3H11_1158 0.45 +0.1 111,057 - Ac3H11_1158 0.45 +1.5 111,057 - Ac3H11_1158 0.45 -2.0 111,215 + Ac3H11_1158 0.69 +1.2 111,215 + Ac3H11_1158 0.69 +0.1 111,216 - Ac3H11_1158 0.70 +0.4 111,216 - Ac3H11_1158 0.70 -1.3 111,216 - Ac3H11_1158 0.70 +0.7 111,320 + Ac3H11_1158 0.85 -0.3 111,320 + Ac3H11_1158 0.85 +1.5 111,491 + +0.3 112,400 + Ac3H11_1159 0.60 -0.5 112,400 + Ac3H11_1159 0.60 -1.3 112,400 + Ac3H11_1159 0.60 +0.4 112,400 + Ac3H11_1159 0.60 +1.7 112,400 + Ac3H11_1159 0.60 +0.9 112,401 - Ac3H11_1159 0.60 -0.3 112,401 - Ac3H11_1159 0.60 -0.3 112,425 + Ac3H11_1159 0.62 -1.9 112,449 + Ac3H11_1159 0.63 -0.2 112,449 + Ac3H11_1159 0.63 -2.8 112,521 + Ac3H11_1159 0.68 +0.6 112,521 + Ac3H11_1159 0.68 +1.0 112,522 - Ac3H11_1159 0.68 -0.6 112,522 - Ac3H11_1159 0.68 +1.4 112,522 - Ac3H11_1159 0.68 -0.2 112,628 - Ac3H11_1159 0.76 -1.6 112,830 + -1.7 112,830 + +1.7 112,830 + -0.4 112,831 - +0.8 112,845 + -0.6 112,982 + -0.6
Or see this region's nucleotide sequence