Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3208 and ksgA are separated by 14 nucleotides ksgA and apaG are separated by 55 nucleotides apaG and apaH are separated by 21 nucleotides apaH and Shewana3_3212 are separated by 96 nucleotides
Shewana3_3208: Shewana3_3208 - 4-hydroxythreonine-4-phosphate dehydrogenase (RefSeq), at 3,852,649 to 3,853,635
_3208
Shewana3_3209: ksgA - dimethyladenosine transferase (RefSeq), at 3,853,650 to 3,854,456
ksgA
Shewana3_3210: apaG - ApaG (RefSeq), at 3,854,512 to 3,854,892
apaG
Shewana3_3211: apaH - diadenosine tetraphosphatase (RefSeq), at 3,854,914 to 3,855,738
apaH
Shewana3_3212: Shewana3_3212 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,855,835 to 3,857,856
_3212
Position (kb)
3854
3855 Strain fitness (log2 ratio)
-1
0
1 at 3854.347 kb on + strand, within ksgA at 3854.495 kb on - strand at 3854.505 kb on - strand at 3854.571 kb on + strand, within apaG at 3854.825 kb on + strand, within apaG at 3854.994 kb on + strand at 3855.312 kb on - strand, within apaH at 3855.338 kb on + strand, within apaH at 3855.413 kb on + strand, within apaH at 3855.759 kb on + strand at 3855.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,854,347 + ksgA Shewana3_3209 0.86 -1.0 3,854,495 - -0.2 3,854,505 - +1.0 3,854,571 + apaG Shewana3_3210 0.15 -0.8 3,854,825 + apaG Shewana3_3210 0.82 -1.3 3,854,994 + -1.3 3,855,312 - apaH Shewana3_3211 0.48 -0.0 3,855,338 + apaH Shewana3_3211 0.51 -1.5 3,855,413 + apaH Shewana3_3211 0.60 -0.3 3,855,759 + -0.0 3,855,759 + -0.2
Or see this region's nucleotide sequence