Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3033 and Shewana3_3034 are separated by 90 nucleotides Shewana3_3034 and Shewana3_3035 overlap by 20 nucleotides Shewana3_3035 and nhaR are separated by 91 nucleotides
Shewana3_3033: Shewana3_3033 - L-aspartate oxidase (RefSeq), at 3,638,013 to 3,639,626
_3033
Shewana3_3034: Shewana3_3034 - hypothetical protein (RefSeq), at 3,639,717 to 3,640,157
_3034
Shewana3_3035: Shewana3_3035 - hypothetical protein (RefSeq), at 3,640,138 to 3,640,377
_3035
Shewana3_3036: nhaR - transcriptional activator NhaR (RefSeq), at 3,640,469 to 3,641,377
nhaR
Position (kb) 3639
3640
3641 Strain fitness (log2 ratio) -2
-1
0
1 at 3639.663 kb on - strand at 3639.670 kb on + strand at 3639.861 kb on + strand, within Shewana3_3034 at 3640.198 kb on + strand, within Shewana3_3035 at 3640.206 kb on - strand, within Shewana3_3035 at 3640.336 kb on - strand, within Shewana3_3035 at 3640.424 kb on + strand at 3640.865 kb on + strand, within nhaR at 3641.137 kb on + strand, within nhaR
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,639,663 - -0.7 3,639,670 + -0.4 3,639,861 + Shewana3_3034 0.33 -0.5 3,640,198 + Shewana3_3035 0.25 -0.9 3,640,206 - Shewana3_3035 0.28 +0.7 3,640,336 - Shewana3_3035 0.82 +0.3 3,640,424 + -2.0 3,640,865 + nhaR Shewana3_3036 0.44 +0.1 3,641,137 + nhaR Shewana3_3036 0.73 +0.4
Or see this region's nucleotide sequence