Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2365 and rnhA are separated by 70 nucleotides rnhA and Shewana3_2367 are separated by 36 nucleotides Shewana3_2367 and Shewana3_2368 are separated by 23 nucleotides
Shewana3_2365: Shewana3_2365 - DNA polymerase III, epsilon subunit (RefSeq), at 2,844,011 to 2,844,739
_2365
Shewana3_2366: rnhA - ribonuclease H (RefSeq), at 2,844,810 to 2,845,286
rnhA
Shewana3_2367: Shewana3_2367 - LysR family transcriptional regulator (RefSeq), at 2,845,323 to 2,846,222
_2367
Shewana3_2368: Shewana3_2368 - methyltransferase type 11 (RefSeq), at 2,846,246 to 2,846,980
_2368
Position (kb)
2844
2845
2846 Strain fitness (log2 ratio)
-2
-1
0
1 at 2843.837 kb on + strand at 2843.837 kb on + strand at 2844.870 kb on - strand, within rnhA at 2845.080 kb on + strand, within rnhA at 2845.433 kb on - strand, within Shewana3_2367 at 2845.438 kb on - strand, within Shewana3_2367 at 2845.471 kb on - strand, within Shewana3_2367 at 2845.506 kb on + strand, within Shewana3_2367
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,843,837 + -1.8 2,843,837 + +0.4 2,844,870 - rnhA Shewana3_2366 0.13 -2.2 2,845,080 + rnhA Shewana3_2366 0.57 -0.9 2,845,433 - Shewana3_2367 0.12 +0.2 2,845,438 - Shewana3_2367 0.13 -1.1 2,845,471 - Shewana3_2367 0.16 -0.9 2,845,506 + Shewana3_2367 0.20 +1.2
Or see this region's nucleotide sequence