Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2363 and Shewana3_R0100 are separated by 282 nucleotides Shewana3_R0100 and Shewana3_R0101 are separated by 56 nucleotides Shewana3_R0101 and Shewana3_2364 are separated by 165 nucleotides Shewana3_2364 and Shewana3_2365 are separated by 358 nucleotides
Shewana3_2363: Shewana3_2363 - cytochrome c552 (RefSeq), at 2,840,377 to 2,841,810
_2363
Shewana3_R0100: Shewana3_R0100 - tRNA-Asp (RefSeq), at 2,842,093 to 2,842,169
_R0100
Shewana3_R0101: Shewana3_R0101 - tRNA-Asp (RefSeq), at 2,842,226 to 2,842,302
_R0101
Shewana3_2364: Shewana3_2364 - hypothetical protein (RefSeq), at 2,842,468 to 2,843,652
_2364
Shewana3_2365: Shewana3_2365 - DNA polymerase III, epsilon subunit (RefSeq), at 2,844,011 to 2,844,739
_2365
Position (kb)
2842
2843
2844 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2841.602 kb on + strand, within Shewana3_2363 at 2841.620 kb on - strand, within Shewana3_2363 at 2841.856 kb on + strand at 2842.574 kb on - strand at 2842.755 kb on + strand, within Shewana3_2364 at 2842.910 kb on - strand, within Shewana3_2364 at 2842.910 kb on - strand, within Shewana3_2364 at 2842.925 kb on - strand, within Shewana3_2364 at 2843.145 kb on - strand, within Shewana3_2364 at 2843.155 kb on - strand, within Shewana3_2364 at 2843.392 kb on - strand, within Shewana3_2364 at 2843.462 kb on + strand, within Shewana3_2364 at 2843.837 kb on + strand at 2843.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,841,602 + Shewana3_2363 0.85 +1.1 2,841,620 - Shewana3_2363 0.87 +1.1 2,841,856 + +2.1 2,842,574 - +0.0 2,842,755 + Shewana3_2364 0.24 +1.4 2,842,910 - Shewana3_2364 0.37 -1.2 2,842,910 - Shewana3_2364 0.37 +0.3 2,842,925 - Shewana3_2364 0.39 +0.3 2,843,145 - Shewana3_2364 0.57 +1.4 2,843,155 - Shewana3_2364 0.58 +1.2 2,843,392 - Shewana3_2364 0.78 +0.7 2,843,462 + Shewana3_2364 0.84 +0.2 2,843,837 + -1.8 2,843,837 + +0.4
Or see this region's nucleotide sequence