Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2330 and Shewana3_2331 are separated by 74 nucleotides Shewana3_2331 and Shewana3_2332 are separated by 6 nucleotides Shewana3_2332 and Shewana3_2333 are separated by 10 nucleotides
Shewana3_2330: Shewana3_2330 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,806,888 to 2,807,898
_2330
Shewana3_2331: Shewana3_2331 - protein tyrosine phosphatase, catalytic region (RefSeq), at 2,807,973 to 2,808,464
_2331
Shewana3_2332: Shewana3_2332 - redox-active disulfide protein 2 (RefSeq), at 2,808,471 to 2,808,707
_2332
Shewana3_2333: Shewana3_2333 - permease (RefSeq), at 2,808,718 to 2,809,716
_2333
Position (kb)
2808
2809 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2807.522 kb on + strand, within Shewana3_2330 at 2807.668 kb on - strand, within Shewana3_2330 at 2807.740 kb on + strand at 2807.847 kb on + strand at 2807.855 kb on - strand at 2808.121 kb on - strand, within Shewana3_2331 at 2808.203 kb on - strand, within Shewana3_2331 at 2808.203 kb on - strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.293 kb on + strand, within Shewana3_2331 at 2808.567 kb on + strand, within Shewana3_2332 at 2808.666 kb on - strand, within Shewana3_2332 at 2808.996 kb on - strand, within Shewana3_2333 at 2809.454 kb on - strand, within Shewana3_2333
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,807,522 + Shewana3_2330 0.63 +2.8 2,807,668 - Shewana3_2330 0.77 -0.5 2,807,740 + +2.8 2,807,847 + -0.5 2,807,855 - -0.0 2,808,121 - Shewana3_2331 0.30 +0.1 2,808,203 - Shewana3_2331 0.47 +0.3 2,808,203 - Shewana3_2331 0.47 -0.3 2,808,293 + Shewana3_2331 0.65 +0.8 2,808,293 + Shewana3_2331 0.65 +1.8 2,808,293 + Shewana3_2331 0.65 -0.5 2,808,567 + Shewana3_2332 0.41 +0.5 2,808,666 - Shewana3_2332 0.82 -0.8 2,808,996 - Shewana3_2333 0.28 -0.0 2,809,454 - Shewana3_2333 0.74 +0.7
Or see this region's nucleotide sequence