Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2119 and Shewana3_2120 are separated by 317 nucleotides Shewana3_2120 and Shewana3_2121 are separated by 164 nucleotides
Shewana3_2119: Shewana3_2119 - mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (RefSeq), at 2,542,351 to 2,543,163
_2119
Shewana3_2120: Shewana3_2120 - hypothetical protein (RefSeq), at 2,543,481 to 2,544,404
_2120
Shewana3_2121: Shewana3_2121 - aromatic amino acid aminotransferase (RefSeq), at 2,544,569 to 2,545,762
_2121
Position (kb)
2543
2544
2545 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2542.568 kb on - strand, within Shewana3_2119 at 2542.601 kb on + strand, within Shewana3_2119 at 2542.774 kb on + strand, within Shewana3_2119 at 2542.846 kb on + strand, within Shewana3_2119 at 2543.124 kb on - strand at 2543.129 kb on - strand at 2543.285 kb on - strand at 2543.640 kb on + strand, within Shewana3_2120 at 2543.645 kb on + strand, within Shewana3_2120 at 2543.803 kb on + strand, within Shewana3_2120 at 2544.258 kb on - strand, within Shewana3_2120 at 2544.333 kb on + strand at 2545.073 kb on + strand, within Shewana3_2121 at 2545.081 kb on - strand, within Shewana3_2121 at 2545.137 kb on + strand, within Shewana3_2121 at 2545.403 kb on - strand, within Shewana3_2121
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,542,568 - Shewana3_2119 0.27 +1.9 2,542,601 + Shewana3_2119 0.31 +1.9 2,542,774 + Shewana3_2119 0.52 +1.9 2,542,846 + Shewana3_2119 0.61 +1.9 2,543,124 - -0.8 2,543,129 - +2.9 2,543,285 - +0.9 2,543,640 + Shewana3_2120 0.17 -0.6 2,543,645 + Shewana3_2120 0.18 -0.6 2,543,803 + Shewana3_2120 0.35 -0.1 2,544,258 - Shewana3_2120 0.84 -0.1 2,544,333 + +1.2 2,545,073 + Shewana3_2121 0.42 +1.3 2,545,081 - Shewana3_2121 0.43 +1.3 2,545,137 + Shewana3_2121 0.48 +0.9 2,545,403 - Shewana3_2121 0.70 -1.1
Or see this region's nucleotide sequence