Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2112 and Shewana3_2113 are separated by 19 nucleotides Shewana3_2113 and Shewana3_2114 are separated by 8 nucleotides Shewana3_2114 and Shewana3_2115 are separated by 55 nucleotides Shewana3_2115 and Shewana3_2116 are separated by 142 nucleotides
Shewana3_2112: Shewana3_2112 - heavy metal translocating P-type ATPase (RefSeq), at 2,535,414 to 2,537,825
_2112
Shewana3_2113: Shewana3_2113 - cytochrome oxidase maturation protein, cbb3-type (RefSeq), at 2,537,845 to 2,538,045
_2113
Shewana3_2114: Shewana3_2114 - hypothetical protein (RefSeq), at 2,538,054 to 2,538,746
_2114
Shewana3_2115: Shewana3_2115 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,538,802 to 2,539,554
_2115
Shewana3_2116: Shewana3_2116 - universal stress protein UspE (RefSeq), at 2,539,697 to 2,540,629
_2116
Position (kb)
2538
2539 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2537.286 kb on - strand, within Shewana3_2112 at 2537.377 kb on + strand, within Shewana3_2112 at 2537.433 kb on + strand, within Shewana3_2112 at 2537.553 kb on + strand, within Shewana3_2112 at 2537.636 kb on + strand at 2538.031 kb on + strand at 2538.163 kb on + strand, within Shewana3_2114 at 2538.387 kb on + strand, within Shewana3_2114 at 2538.516 kb on - strand, within Shewana3_2114 at 2538.536 kb on - strand, within Shewana3_2114 at 2538.748 kb on - strand at 2539.154 kb on + strand, within Shewana3_2115 at 2539.554 kb on + strand at 2539.666 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,537,286 - Shewana3_2112 0.78 +1.8 2,537,377 + Shewana3_2112 0.81 +1.1 2,537,433 + Shewana3_2112 0.84 +1.8 2,537,553 + Shewana3_2112 0.89 +2.1 2,537,636 + +2.1 2,538,031 + +1.7 2,538,163 + Shewana3_2114 0.16 +3.0 2,538,387 + Shewana3_2114 0.48 +2.2 2,538,516 - Shewana3_2114 0.67 +1.8 2,538,536 - Shewana3_2114 0.70 +1.8 2,538,748 - +1.9 2,539,154 + Shewana3_2115 0.47 +0.6 2,539,554 + +1.0 2,539,666 + +1.9
Or see this region's nucleotide sequence