Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1960 and Shewana3_1961 are separated by 8 nucleotides Shewana3_1961 and Shewana3_1962 overlap by 4 nucleotides Shewana3_1962 and ansA are separated by 70 nucleotides ansA and Shewana3_1964 are separated by 111 nucleotides
Shewana3_1960: Shewana3_1960 - peptidase M14, carboxypeptidase A (RefSeq), at 2,333,947 to 2,335,074
_1960
Shewana3_1961: Shewana3_1961 - hypothetical protein (RefSeq), at 2,335,083 to 2,335,400
_1961
Shewana3_1962: Shewana3_1962 - hypothetical protein (RefSeq), at 2,335,397 to 2,335,678
_1962
Shewana3_1963: ansA - cytoplasmic asparaginase I (RefSeq), at 2,335,749 to 2,336,762
ansA
Shewana3_1964: Shewana3_1964 - signal peptide peptidase SppA, 67K type (RefSeq), at 2,336,874 to 2,338,718
_1964
Position (kb)
2335
2336
2337 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2334.952 kb on - strand, within Shewana3_1960 at 2335.024 kb on + strand at 2335.558 kb on + strand, within Shewana3_1962 at 2335.845 kb on - strand at 2335.938 kb on - strand, within ansA at 2336.041 kb on - strand, within ansA at 2336.150 kb on - strand, within ansA at 2336.215 kb on + strand, within ansA at 2336.463 kb on + strand at 2336.541 kb on - strand, within ansA at 2336.760 kb on + strand at 2336.920 kb on - strand at 2336.981 kb on + strand at 2337.126 kb on + strand, within Shewana3_1964 at 2337.500 kb on + strand, within Shewana3_1964
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,334,952 - Shewana3_1960 0.89 -0.8 2,335,024 + +2.2 2,335,558 + Shewana3_1962 0.57 +1.8 2,335,845 - +0.6 2,335,938 - ansA Shewana3_1963 0.19 -0.2 2,336,041 - ansA Shewana3_1963 0.29 -0.6 2,336,150 - ansA Shewana3_1963 0.40 +0.4 2,336,215 + ansA Shewana3_1963 0.46 +0.1 2,336,463 + +0.6 2,336,541 - ansA Shewana3_1963 0.78 -0.4 2,336,760 + +0.9 2,336,920 - +1.1 2,336,981 + +2.9 2,337,126 + Shewana3_1964 0.14 +2.9 2,337,500 + Shewana3_1964 0.34 +1.9
Or see this region's nucleotide sequence