Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1959 and Shewana3_1960 are separated by 180 nucleotides Shewana3_1960 and Shewana3_1961 are separated by 8 nucleotides Shewana3_1961 and Shewana3_1962 overlap by 4 nucleotides Shewana3_1962 and ansA are separated by 70 nucleotides
Shewana3_1959: Shewana3_1959 - two component transcriptional regulator (RefSeq), at 2,333,086 to 2,333,766
_1959
Shewana3_1960: Shewana3_1960 - peptidase M14, carboxypeptidase A (RefSeq), at 2,333,947 to 2,335,074
_1960
Shewana3_1961: Shewana3_1961 - hypothetical protein (RefSeq), at 2,335,083 to 2,335,400
_1961
Shewana3_1962: Shewana3_1962 - hypothetical protein (RefSeq), at 2,335,397 to 2,335,678
_1962
Shewana3_1963: ansA - cytoplasmic asparaginase I (RefSeq), at 2,335,749 to 2,336,762
ansA
Position (kb)
2333
2334
2335
2336 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2333.000 kb on - strand at 2333.194 kb on - strand, within Shewana3_1959 at 2333.213 kb on + strand, within Shewana3_1959 at 2333.291 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.432 kb on + strand, within Shewana3_1959 at 2333.705 kb on + strand at 2333.901 kb on + strand at 2334.136 kb on + strand, within Shewana3_1960 at 2334.183 kb on + strand, within Shewana3_1960 at 2334.238 kb on + strand, within Shewana3_1960 at 2334.277 kb on - strand, within Shewana3_1960 at 2334.285 kb on - strand, within Shewana3_1960 at 2334.357 kb on + strand, within Shewana3_1960 at 2334.632 kb on + strand, within Shewana3_1960 at 2334.952 kb on - strand, within Shewana3_1960 at 2335.024 kb on + strand at 2335.558 kb on + strand, within Shewana3_1962 at 2335.845 kb on - strand at 2335.938 kb on - strand, within ansA at 2336.041 kb on - strand, within ansA
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,333,000 - -0.1 2,333,194 - Shewana3_1959 0.16 +1.9 2,333,213 + Shewana3_1959 0.19 -1.6 2,333,291 + Shewana3_1959 0.30 +1.9 2,333,432 + Shewana3_1959 0.51 -0.1 2,333,432 + Shewana3_1959 0.51 +1.5 2,333,705 + +0.5 2,333,901 + +0.5 2,334,136 + Shewana3_1960 0.17 +2.1 2,334,183 + Shewana3_1960 0.21 +1.3 2,334,238 + Shewana3_1960 0.26 +2.6 2,334,277 - Shewana3_1960 0.29 +2.9 2,334,285 - Shewana3_1960 0.30 +2.9 2,334,357 + Shewana3_1960 0.36 -0.4 2,334,632 + Shewana3_1960 0.61 +0.9 2,334,952 - Shewana3_1960 0.89 -0.8 2,335,024 + +2.2 2,335,558 + Shewana3_1962 0.57 +1.8 2,335,845 - +0.6 2,335,938 - ansA Shewana3_1963 0.19 -0.2 2,336,041 - ansA Shewana3_1963 0.29 -0.6
Or see this region's nucleotide sequence