Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1745 and Shewana3_1746 are separated by 29 nucleotides Shewana3_1746 and mnmA are separated by 3 nucleotides mnmA and Shewana3_1748 are separated by 221 nucleotides Shewana3_1748 and Shewana3_1749 are separated by 73 nucleotides
Shewana3_1745: Shewana3_1745 - adenylosuccinate lyase (RefSeq), at 2,058,797 to 2,060,167
_1745
Shewana3_1746: Shewana3_1746 - hypothetical protein (RefSeq), at 2,060,197 to 2,060,814
_1746
Shewana3_1747: mnmA - tRNA-specific 2-thiouridylase MnmA (RefSeq), at 2,060,818 to 2,061,936
mnmA
Shewana3_1748: Shewana3_1748 - NUDIX hydrolase (RefSeq), at 2,062,158 to 2,062,649
_1748
Shewana3_1749: Shewana3_1749 - ribosomal large subunit pseudouridine synthase E (RefSeq), at 2,062,723 to 2,063,532
_1749
Position (kb)
2060
2061
2062 Strain fitness (log2 ratio)
-1
0
1 at 2060.625 kb on - strand, within Shewana3_1746 at 2060.642 kb on - strand, within Shewana3_1746 at 2060.647 kb on - strand, within Shewana3_1746 at 2061.995 kb on - strand at 2062.212 kb on - strand, within Shewana3_1748 at 2062.240 kb on + strand, within Shewana3_1748 at 2062.348 kb on + strand, within Shewana3_1748 at 2062.348 kb on + strand, within Shewana3_1748 at 2062.496 kb on - strand, within Shewana3_1748 at 2062.598 kb on + strand, within Shewana3_1748 at 2062.678 kb on + strand at 2062.793 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,060,625 - Shewana3_1746 0.69 +1.4 2,060,642 - Shewana3_1746 0.72 -0.1 2,060,647 - Shewana3_1746 0.73 +1.2 2,061,995 - +0.8 2,062,212 - Shewana3_1748 0.11 +1.7 2,062,240 + Shewana3_1748 0.17 +0.2 2,062,348 + Shewana3_1748 0.39 -0.7 2,062,348 + Shewana3_1748 0.39 +0.7 2,062,496 - Shewana3_1748 0.69 -0.1 2,062,598 + Shewana3_1748 0.89 +1.1 2,062,678 + -0.4 2,062,793 + -1.4
Or see this region's nucleotide sequence