Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1314 and Shewana3_1315 overlap by 4 nucleotides Shewana3_1315 and Shewana3_1316 are separated by 102 nucleotides Shewana3_1316 and Shewana3_1317 are separated by 2 nucleotides Shewana3_1317 and flgA are separated by 75 nucleotides flgA and Shewana3_1319 are separated by 205 nucleotides
Shewana3_1314: Shewana3_1314 - hypothetical protein (RefSeq), at 1,529,551 to 1,530,183
_1314
Shewana3_1315: Shewana3_1315 - hypothetical protein (RefSeq), at 1,530,180 to 1,530,629
_1315
Shewana3_1316: Shewana3_1316 - FlgN family protein (RefSeq), at 1,530,732 to 1,531,157
_1316
Shewana3_1317: Shewana3_1317 - anti-sigma-28 factor, FlgM (RefSeq), at 1,531,160 to 1,531,486
_1317
Shewana3_1318: flgA - flagellar basal body P-ring biosynthesis protein FlgA (RefSeq), at 1,531,562 to 1,532,269
flgA
Shewana3_1319: Shewana3_1319 - response regulator receiver modulated CheW protein (RefSeq), at 1,532,475 to 1,533,395
_1319
Position (kb)
1531
1532 Strain fitness (log2 ratio)
-2
-1
0
1 at 1530.202 kb on - strand at 1530.472 kb on - strand, within Shewana3_1315 at 1530.496 kb on + strand, within Shewana3_1315 at 1530.593 kb on - strand at 1530.638 kb on - strand at 1530.658 kb on + strand at 1531.098 kb on - strand, within Shewana3_1316 at 1531.133 kb on - strand at 1531.196 kb on - strand, within Shewana3_1317 at 1531.336 kb on - strand, within Shewana3_1317 at 1531.520 kb on - strand at 1531.623 kb on - strand at 1531.671 kb on + strand, within flgA at 1531.894 kb on - strand, within flgA at 1531.894 kb on - strand, within flgA at 1532.002 kb on + strand, within flgA at 1532.096 kb on + strand, within flgA at 1532.278 kb on + strand at 1532.319 kb on + strand at 1532.329 kb on + strand at 1532.329 kb on + strand at 1532.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,530,202 - +0.1 1,530,472 - Shewana3_1315 0.65 -1.7 1,530,496 + Shewana3_1315 0.70 -0.0 1,530,593 - -0.2 1,530,638 - -0.9 1,530,658 + -0.2 1,531,098 - Shewana3_1316 0.86 +0.3 1,531,133 - +0.8 1,531,196 - Shewana3_1317 0.11 +0.2 1,531,336 - Shewana3_1317 0.54 +1.7 1,531,520 - -0.0 1,531,623 - -2.7 1,531,671 + flgA Shewana3_1318 0.15 -1.6 1,531,894 - flgA Shewana3_1318 0.47 -1.0 1,531,894 - flgA Shewana3_1318 0.47 -0.5 1,532,002 + flgA Shewana3_1318 0.62 -0.2 1,532,096 + flgA Shewana3_1318 0.75 -1.2 1,532,278 + +0.1 1,532,319 + +0.3 1,532,329 + +0.1 1,532,329 + -0.3 1,532,344 - -0.0
Or see this region's nucleotide sequence