Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1282 and Shewana3_1283 overlap by 4 nucleotides Shewana3_1283 and Shewana3_1284 overlap by 4 nucleotides Shewana3_1284 and Shewana3_1285 are separated by 10 nucleotides
Shewana3_1282: Shewana3_1282 - type II secretion system protein E (RefSeq), at 1,493,533 to 1,494,603
_1282
Shewana3_1283: Shewana3_1283 - conjugal transfer protein TrbC (RefSeq), at 1,494,600 to 1,494,986
_1283
Shewana3_1284: Shewana3_1284 - putative conjugal transfer trbD transmembrane protein (RefSeq), at 1,494,983 to 1,495,252
_1284
Shewana3_1285: Shewana3_1285 - conjugal transfer ATPase TrbE (RefSeq), at 1,495,263 to 1,497,713
_1285
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-1
0
1 at 1494.051 kb on + strand, within Shewana3_1282 at 1494.423 kb on + strand, within Shewana3_1282 at 1494.481 kb on - strand, within Shewana3_1282 at 1494.558 kb on - strand at 1494.668 kb on + strand, within Shewana3_1283 at 1494.685 kb on + strand, within Shewana3_1283 at 1495.041 kb on + strand, within Shewana3_1284 at 1495.049 kb on - strand, within Shewana3_1284 at 1495.124 kb on + strand, within Shewana3_1284 at 1495.144 kb on + strand, within Shewana3_1284 at 1495.371 kb on - strand at 1495.398 kb on + strand at 1495.398 kb on + strand at 1495.398 kb on + strand at 1495.406 kb on - strand at 1495.406 kb on - strand at 1495.422 kb on + strand at 1495.763 kb on + strand, within Shewana3_1285 at 1495.771 kb on - strand, within Shewana3_1285 at 1495.802 kb on - strand, within Shewana3_1285 at 1495.977 kb on + strand, within Shewana3_1285 at 1496.045 kb on - strand, within Shewana3_1285 at 1496.065 kb on - strand, within Shewana3_1285 at 1496.077 kb on - strand, within Shewana3_1285
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,494,051 + Shewana3_1282 0.48 +1.3 1,494,423 + Shewana3_1282 0.83 +0.4 1,494,481 - Shewana3_1282 0.89 +0.3 1,494,558 - +0.4 1,494,668 + Shewana3_1283 0.18 +0.3 1,494,685 + Shewana3_1283 0.22 -0.8 1,495,041 + Shewana3_1284 0.21 +0.4 1,495,049 - Shewana3_1284 0.24 +0.3 1,495,124 + Shewana3_1284 0.52 +0.8 1,495,144 + Shewana3_1284 0.60 +1.5 1,495,371 - +0.4 1,495,398 + +0.4 1,495,398 + +0.5 1,495,398 + +0.2 1,495,406 - -0.1 1,495,406 - -0.6 1,495,422 + +0.8 1,495,763 + Shewana3_1285 0.20 -0.1 1,495,771 - Shewana3_1285 0.21 -0.1 1,495,802 - Shewana3_1285 0.22 +0.1 1,495,977 + Shewana3_1285 0.29 +0.3 1,496,045 - Shewana3_1285 0.32 +0.3 1,496,065 - Shewana3_1285 0.33 -0.2 1,496,077 - Shewana3_1285 0.33 -0.2
Or see this region's nucleotide sequence