Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoA and Shewana3_1043 are separated by 9 nucleotides Shewana3_1043 and deoD are separated by 91 nucleotides deoD and Shewana3_1045 are separated by 167 nucleotides
Shewana3_1042: deoA - thymidine phosphorylase (RefSeq), at 1,218,945 to 1,220,276
deoA
Shewana3_1043: Shewana3_1043 - phosphopentomutase (RefSeq), at 1,220,286 to 1,221,500
_1043
Shewana3_1044: deoD - purine nucleoside phosphorylase (RefSeq), at 1,221,592 to 1,222,302
deoD
Shewana3_1045: Shewana3_1045 - membrane protein (RefSeq), at 1,222,470 to 1,223,654
_1045
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1219.599 kb on - strand, within deoA at 1219.661 kb on - strand, within deoA at 1219.677 kb on + strand, within deoA at 1219.753 kb on - strand, within deoA at 1219.757 kb on + strand, within deoA at 1219.824 kb on + strand, within deoA at 1220.002 kb on + strand, within deoA at 1220.019 kb on - strand, within deoA at 1220.091 kb on - strand, within deoA at 1220.099 kb on + strand, within deoA at 1220.159 kb on + strand at 1220.243 kb on + strand at 1220.306 kb on - strand at 1220.458 kb on + strand, within Shewana3_1043 at 1220.471 kb on - strand, within Shewana3_1043 at 1220.632 kb on + strand, within Shewana3_1043 at 1220.760 kb on + strand, within Shewana3_1043 at 1220.763 kb on + strand, within Shewana3_1043 at 1220.892 kb on + strand, within Shewana3_1043 at 1221.280 kb on - strand, within Shewana3_1043 at 1221.836 kb on + strand, within deoD at 1221.884 kb on + strand, within deoD at 1222.020 kb on + strand, within deoD at 1222.076 kb on - strand at 1222.144 kb on + strand, within deoD at 1222.442 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,219,599 - deoA Shewana3_1042 0.49 -1.7 1,219,661 - deoA Shewana3_1042 0.54 -1.7 1,219,677 + deoA Shewana3_1042 0.55 +1.6 1,219,753 - deoA Shewana3_1042 0.61 -0.2 1,219,757 + deoA Shewana3_1042 0.61 +0.2 1,219,824 + deoA Shewana3_1042 0.66 +1.1 1,220,002 + deoA Shewana3_1042 0.79 +0.9 1,220,019 - deoA Shewana3_1042 0.81 -0.4 1,220,091 - deoA Shewana3_1042 0.86 -1.9 1,220,099 + deoA Shewana3_1042 0.87 +0.6 1,220,159 + +0.9 1,220,243 + +1.2 1,220,306 - -1.2 1,220,458 + Shewana3_1043 0.14 +0.8 1,220,471 - Shewana3_1043 0.15 -2.0 1,220,632 + Shewana3_1043 0.28 +1.1 1,220,760 + Shewana3_1043 0.39 +1.1 1,220,763 + Shewana3_1043 0.39 +1.2 1,220,892 + Shewana3_1043 0.50 +1.2 1,221,280 - Shewana3_1043 0.82 +2.0 1,221,836 + deoD Shewana3_1044 0.34 +0.7 1,221,884 + deoD Shewana3_1044 0.41 +0.9 1,222,020 + deoD Shewana3_1044 0.60 +0.9 1,222,076 - +3.0 1,222,144 + deoD Shewana3_1044 0.78 +1.3 1,222,442 - +0.8
Or see this region's nucleotide sequence