Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0723 and Shewana3_0724 are separated by 44 nucleotides Shewana3_0724 and tolC are separated by 72 nucleotides
Shewana3_0723: Shewana3_0723 - hypothetical protein (RefSeq), at 839,748 to 840,716
_0723
Shewana3_0724: Shewana3_0724 - hypothetical protein (RefSeq), at 840,761 to 841,456
_0724
Shewana3_0725: tolC - outer membrane channel protein (RefSeq), at 841,529 to 842,836
tolC
Position (kb) 840
841
842 Strain fitness (log2 ratio) -2
-1
0
1
2 at 839.909 kb on + strand, within Shewana3_0723 at 840.149 kb on + strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.186 kb on - strand, within Shewana3_0723 at 840.272 kb on + strand, within Shewana3_0723 at 840.549 kb on - strand, within Shewana3_0723 at 840.593 kb on - strand, within Shewana3_0723 at 840.615 kb on + strand, within Shewana3_0723 at 840.675 kb on + strand at 840.746 kb on + strand at 840.746 kb on + strand at 840.754 kb on - strand at 840.890 kb on + strand, within Shewana3_0724 at 840.995 kb on + strand, within Shewana3_0724 at 841.054 kb on - strand, within Shewana3_0724 at 841.072 kb on + strand, within Shewana3_0724 at 841.092 kb on + strand, within Shewana3_0724 at 841.121 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.220 kb on + strand, within Shewana3_0724 at 841.313 kb on + strand, within Shewana3_0724 at 841.671 kb on - strand, within tolC at 841.671 kb on - strand, within tolC at 841.770 kb on - strand, within tolC at 841.839 kb on - strand, within tolC at 841.894 kb on + strand, within tolC at 842.002 kb on - strand, within tolC at 842.252 kb on - strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 839,909 + Shewana3_0723 0.17 -0.0 840,149 + Shewana3_0723 0.41 -1.1 840,186 - Shewana3_0723 0.45 -1.4 840,186 - Shewana3_0723 0.45 -1.4 840,272 + Shewana3_0723 0.54 +0.5 840,549 - Shewana3_0723 0.83 -2.2 840,593 - Shewana3_0723 0.87 +1.8 840,615 + Shewana3_0723 0.89 +1.3 840,675 + +0.7 840,746 + +0.8 840,746 + +0.8 840,754 - +0.8 840,890 + Shewana3_0724 0.19 +1.2 840,995 + Shewana3_0724 0.34 +1.0 841,054 - Shewana3_0724 0.42 +1.8 841,072 + Shewana3_0724 0.45 +1.1 841,092 + Shewana3_0724 0.48 +0.1 841,121 + Shewana3_0724 0.52 +1.3 841,220 + Shewana3_0724 0.66 +1.0 841,220 + Shewana3_0724 0.66 +1.1 841,313 + Shewana3_0724 0.79 +1.4 841,671 - tolC Shewana3_0725 0.11 +0.8 841,671 - tolC Shewana3_0725 0.11 +0.4 841,770 - tolC Shewana3_0725 0.18 +0.3 841,839 - tolC Shewana3_0725 0.24 -0.3 841,894 + tolC Shewana3_0725 0.28 +1.8 842,002 - tolC Shewana3_0725 0.36 +0.9 842,252 - tolC Shewana3_0725 0.55 +0.7
Or see this region's nucleotide sequence