Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0518 and Shewana3_0519 are separated by 129 nucleotides Shewana3_0519 and mscL are separated by 82 nucleotides mscL and Shewana3_0521 are separated by 184 nucleotides
Shewana3_0518: Shewana3_0518 - CzcA family heavy metal efflux protein (RefSeq), at 607,495 to 610,707
_0518
Shewana3_0519: Shewana3_0519 - antibiotic biosynthesis monooxygenase (RefSeq), at 610,837 to 611,160
_0519
Shewana3_0520: mscL - large-conductance mechanosensitive channel (RefSeq), at 611,243 to 611,653
mscL
Shewana3_0521: Shewana3_0521 - LysR family transcriptional regulator (RefSeq), at 611,838 to 612,791
_0521
Position (kb)
611
612 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 610.268 kb on + strand, within Shewana3_0518 at 610.439 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.528 kb on + strand at 610.560 kb on - strand at 610.568 kb on + strand at 610.574 kb on - strand at 610.632 kb on - strand at 610.639 kb on + strand at 610.647 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.743 kb on + strand at 610.930 kb on + strand, within Shewana3_0519 at 610.984 kb on + strand, within Shewana3_0519 at 611.031 kb on - strand, within Shewana3_0519 at 611.119 kb on + strand, within Shewana3_0519 at 611.127 kb on - strand, within Shewana3_0519 at 611.140 kb on + strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.267 kb on - strand at 611.464 kb on - strand, within mscL at 611.512 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.557 kb on - strand, within mscL at 611.636 kb on - strand at 611.911 kb on + strand at 611.911 kb on + strand at 611.911 kb on + strand at 611.929 kb on - strand at 611.961 kb on + strand, within Shewana3_0521 at 612.003 kb on - strand, within Shewana3_0521 at 612.143 kb on + strand, within Shewana3_0521 at 612.166 kb on + strand, within Shewana3_0521 at 612.381 kb on + strand, within Shewana3_0521 at 612.395 kb on + strand, within Shewana3_0521 at 612.464 kb on + strand, within Shewana3_0521 at 612.486 kb on + strand, within Shewana3_0521 at 612.631 kb on - strand, within Shewana3_0521
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 610,268 + Shewana3_0518 0.86 +0.2 610,439 + +0.2 610,528 + +0.5 610,528 + +0.8 610,528 + +0.8 610,560 - -1.0 610,568 + +0.3 610,574 - -2.8 610,632 - -0.1 610,639 + +0.2 610,647 - -1.5 610,710 - -1.3 610,710 - -3.0 610,710 - -1.1 610,743 + +0.7 610,930 + Shewana3_0519 0.29 +0.9 610,984 + Shewana3_0519 0.45 -0.7 611,031 - Shewana3_0519 0.60 -0.2 611,119 + Shewana3_0519 0.87 +0.5 611,127 - Shewana3_0519 0.90 -0.5 611,140 + +0.6 611,267 - +1.1 611,267 - +0.7 611,267 - +1.1 611,464 - mscL Shewana3_0520 0.54 +0.5 611,512 - mscL Shewana3_0520 0.65 +0.8 611,557 - mscL Shewana3_0520 0.76 +0.7 611,557 - mscL Shewana3_0520 0.76 +0.8 611,636 - -0.3 611,911 + -1.5 611,911 + +1.4 611,911 + -0.2 611,929 - -0.6 611,961 + Shewana3_0521 0.13 -0.5 612,003 - Shewana3_0521 0.17 -1.1 612,143 + Shewana3_0521 0.32 -1.6 612,166 + Shewana3_0521 0.34 +0.1 612,381 + Shewana3_0521 0.57 +0.5 612,395 + Shewana3_0521 0.58 -0.1 612,464 + Shewana3_0521 0.66 +0.0 612,486 + Shewana3_0521 0.68 -0.4 612,631 - Shewana3_0521 0.83 -1.0
Or see this region's nucleotide sequence