Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recF and Shewana3_0012 are separated by 16 nucleotides Shewana3_0012 and Shewana3_0013 are separated by 82 nucleotides Shewana3_0013 and Shewana3_0014 are separated by 126 nucleotides
Shewana3_0011: recF - recombination protein F (RefSeq), at 12,317 to 13,399
recF
Shewana3_0012: Shewana3_0012 - DNA gyrase subunit B (RefSeq), at 13,416 to 15,833
_0012
Shewana3_0013: Shewana3_0013 - glutathione S-transferase domain-containing protein (RefSeq), at 15,916 to 16,572
_0013
Shewana3_0014: Shewana3_0014 - transcriptional regulator (RefSeq), at 16,699 to 17,547
_0014
Position (kb) 13
14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 12.433 kb on + strand, within recF at 12.511 kb on + strand, within recF at 12.519 kb on - strand, within recF at 12.524 kb on - strand, within recF at 12.541 kb on - strand, within recF at 12.542 kb on - strand, within recF at 12.877 kb on + strand, within recF at 12.885 kb on - strand, within recF at 12.895 kb on + strand, within recF at 12.922 kb on + strand, within recF at 12.930 kb on - strand, within recF at 12.967 kb on + strand, within recF at 13.005 kb on + strand, within recF at 13.042 kb on + strand, within recF at 16.131 kb on - strand, within Shewana3_0013 at 16.131 kb on - strand, within Shewana3_0013 at 16.247 kb on - strand, within Shewana3_0013 at 16.480 kb on + strand, within Shewana3_0013 at 16.598 kb on - strand at 16.824 kb on - strand, within Shewana3_0014
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 12,433 + recF Shewana3_0011 0.11 -1.8 12,511 + recF Shewana3_0011 0.18 -2.1 12,519 - recF Shewana3_0011 0.19 -3.2 12,524 - recF Shewana3_0011 0.19 -2.4 12,541 - recF Shewana3_0011 0.21 -1.2 12,542 - recF Shewana3_0011 0.21 -1.4 12,877 + recF Shewana3_0011 0.52 -1.2 12,885 - recF Shewana3_0011 0.52 -2.1 12,895 + recF Shewana3_0011 0.53 -1.7 12,922 + recF Shewana3_0011 0.56 -1.2 12,930 - recF Shewana3_0011 0.57 -0.1 12,967 + recF Shewana3_0011 0.60 -0.2 13,005 + recF Shewana3_0011 0.64 -1.0 13,042 + recF Shewana3_0011 0.67 -2.5 16,131 - Shewana3_0013 0.33 -0.0 16,131 - Shewana3_0013 0.33 +0.1 16,247 - Shewana3_0013 0.50 +0.4 16,480 + Shewana3_0013 0.86 +0.6 16,598 - -0.3 16,824 - Shewana3_0014 0.15 -0.7
Or see this region's nucleotide sequence